코엔자임 Q10 처리에 따른 TEGDMA에 의해 유발된 치아세포 사멸 억제 효과
The protective effect of coenzyme Q10 on cytotoxicity of regin monomer of odontoblast caused by TEGDMA
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Objectives : The purpose of the study is to investigate the protective effect of coenzyme Q10 on cytotoxicity effect of dental monomers in odontoblast(MDPC-23). Methods : MDPC-23 was incubated with the(co)monomers triethylene glycol dimethacrylate (TEGDMA) with and without addition of coenzyme Q10. The cell proliferation and survival was determined using WST-1 assay. The level of reactive oxygen species(ROS) was measured by immunofluorescent staining for DCF-DA. Results : TEGDMA treatment decreased the cell proliferation by dose dependently(0.1, 1, 2.5, 5, 10 mM) on the growth of MDPC-23 cells. Coenzyme Q10 showed cell proliferation from 5 to 500 µM by WST-1 assay. Pre-treatment coenzyme Q10 showed the antioxidant effect on proliferation and viability of MDPC-23 after 48h(p<0.05). The positive cells were observed in non-coenyme Q10 treatment group(group 2) in comparison with coenyme Q10 pre-treatment group(group 1) by DCF-DA. The fluorescence positive cells showed 14.715(group 1) and 19.788(group 2) using image J system. Conclusions : TEGDMA induced cytotoxicity. The MDPC-23 cell death was associated with the increasing ROS. Coenyme Q10 showed the antioxidant effects by decreasing ROS. This effects may contribute to the treatment of periodontal disease induced by TEGDMA after operation.
Abstract
Objectives : The purpose of the study is to investigate the protective effect of coenzyme Q10 on cytotoxicity effect of dental monomers in odontoblast(MDPC-23). Methods : MDPC-23 was incubated with the(co)monomers triethylene glycol dimethacrylate (TEGDMA) with and without addition of coenzyme Q10. The cell proliferation and survival was determined using WST-1 assay. The level of reactive oxygen species(ROS) was measured by immunofluorescent staining for DCF-DA. Results : TEGDMA treatment decreased the cell proliferation by dose dependently(0.1, 1, 2.5, 5, 10 mM) on the growth of MDPC-23 cells. Coenzyme Q10 showed cell proliferation from 5 to 500 µM by WST-1 assay. Pre-treatment coenzyme Q10 showed the antioxidant effect on proliferation and viability of MDPC-23 after 48h(p<0.05). The positive cells were observed in non-coenyme Q10 treatment group(group 2) in comparison with coenyme Q10 pre-treatment group(group 1) by DCF-DA. The fluorescence positive cells showed 14.715(group 1) and 19.788(group 2) using image J system. Conclusions : TEGDMA induced cytotoxicity. The MDPC-23 cell death was associated with the increasing ROS. Coenyme Q10 showed the antioxidant effects by decreasing ROS. This effects may contribute to the treatment of periodontal disease induced by TEGDMA after operation.
참고문헌 (28)
- 이연경, "연령에 따른 비만과 치주질환과의 관련성", 한국치위생학회지, 13(6), pp.1015~1021 (2013), 한국치위생학회[DOI]
- 최별보라, "에탄올에 의해 추출한 황금이 구강암 세포에서 나타나는 자가포식작용", 한국치위생학회지, 14(2), pp.287~292 (2014), 한국치위생학회[DOI]
- Ferracane JL, "Current trends in dental composites", Crit Rev Oral Biol Med, 6(4), pp.302~318 (1995)[DOI]
- Issa Y, "Resin composite monomers alter MTT and LDH activity of human gingival fibroblasts in vitro", Dent Mater, 20(1), pp.12~20 (2004)[DOI]
- Bakopoulou A, "Molecular toxicology of substances released from resin-based dental restorative materials", Int J Mol Sci, 10(9), pp.3961~3999 (2009)[DOI]
- Engelmann J, "Metabolic effects of dental resin components in vitro detected by NMR spectroscopy", J Dent Res, 80(3), pp.869~875 (2001)[DOI]
- Stanislawski L, "TEGDMA-induced toxicity in human fibroblasts is associated with early and drastic glutathione depletion with subsequent production of oxygen reactive species", J Biomed Mater Res A, 66(3), pp.476~482 (2003)[DOI]
- 장종화, "매실추출물(PME)의 구강 미생물에 대한 항균작용", 한국치위생학회지, 14(1), pp.109~115 (2014), 한국치위생학회[DOI]
- Schweikl H, "The mutagenic activity of unpolymerized resin monomers in Salmonella typhimurium and V79 cells", Mutat Res, 415(1-2), pp.119~130 (1998)[DOI]
- Kleinsasser NH, "Genotoxicity and cytotoxicity of dental materials in human lymphocytes as assessed by the single cell microgel electrophoresis (comet) assay", J Dent, 32(3), pp.229~234 (2004)[DOI]
- Lefeuvre M, "TEGDMA induces mitochondrial damage and oxidative stress in human gingival fibroblasts", Biomaterials, 26, pp.5130~5137 (2005)[DOI]
- Rajendran P, "Antioxidants and human diseases", Clin Chim Acta, 436(25), pp.332~347 (2014)[DOI]
- Chen SJ, "Influence of reactive oxygen species on human sperm functions and fertilizing capacity including therapeutical approaches", Arch Gynecol Obstet, 288(1), pp.191~199 (2013)[DOI]
- Tanja Schaffer, "Increase of Bioavailability of Coenzyme Q10 and Vitamin E", Journal of Medicinal Food, 10(4), pp.731~734 (2007), 한국식품영양과학회[DOI]
- Young AJ, "Coenzyme Q10: a review of its promise as a neuroprotectant", CNS Spectr, 12(1), pp.62~68 (2007)
- Engel RH1, "Oxidative stress and apoptosis: a new treatment paradigm in cancer", Front Biosci, 11, pp.300~312 (2006)
- Brock GR, "Local and systemic total antioxidant capacity in periodontitis and health", J Clin Periodontol, 31(7), pp.515~521 (2004)[DOI]
- Kuzmanova D, "Vitamin C in plasma and leucocytes in relation to periodontitis", J Clin Periodontol, 39(10), pp.905~912 (2012)[DOI]
- Wilkinson EG, "Bioenergetics in clinical medicine. VI. adjunctive treatment of periodontal disease with coenzyme Q10", Res Commun Chem Pathol Pharmacol, 14(4), pp.715~719 (1976)
- Chang HH, "Effect of triethylene glycol dimethacrylate on the cytotoxicity, cyclooxygenase-2 expression and prostanoids production in human dental pulp cells", Int Endod J, 45(9), pp.848~858 (2012)[DOI]
- Schweikl H, "The effect of triethylene glycol dimethacrylate on the cell cycle of mammalian cells", Biomaterials, 26(19), pp.4111~4118 (2005)[DOI]
- Janke V, "TEGDMA causes apoptosis in primary human gingival fibroblasts", J Dent Res, 82(10), pp.814~818 (2003)[DOI]
- Turunen M, "Metabolism and function of coenzyme Q", Biochim Biophys Acta, 1660(1-2), pp.171~199 (2004)[DOI]
- Hanioka T, "Effect of topical application of coenzyme Q10 on adult periodontitis", Mol Aspects Med, 15, pp.s241~s248 (1994)
- Wang S, "Protective effect of Coenzyme Q(10) against oxidative damage in human lens epithelial cells by novel ocular drug carriers", Int J Pharm, 403(1-2), pp.219~229 (2011)[DOI]
- Noh YH, "Inhibition of oxidative stress by coenzyme Q10 increases mitochondrial mass and improves bioenergetic function in optic nerve head astrocytes", Cell Death Dis, 4, pp.e820~ (2013)[DOI]
- Hans M, "Clinical evaluation of topical application of perio-Q gel (Coenzyme Q(10)) in chronic periodontitis patients", J Indian Soc Periodontol, 16(2), pp.193~199 (2012)[DOI]
- Lottner S, "Effects of antioxidants on DNA-double strand breaks in human gingival fibroblasts exposed to methacrylate based monomers", Dent Mater, 29(9), pp.991~998 (2013)[DOI]