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간접수용복 시멘트 처리로 유발된 활성산소종에 의한 치주줄기세포 독성

Reactive oxygen species-mediated cytotoxicity of indirect restorative cement on periodontal stem cells

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Objectives: This study aimed to investigate the cytotoxicity of Nexus RMGIC, an indirect restorative cement, on cell survival rate and reactive oxygen species (ROS) production in periodontal stem cells (PDSCs). Methods: PDSCs were incubated with serially diluted Nexus RMGIC eluates with and without the addition of N-acetyl-cysteine (NAC). Cell survival was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The ROS generation was determined by measuring the fluorescence intensity for 2′,7′-dichlorofluorescin diacetate. Results: Nexus RMGIC exposure decreased cell proliferation and cell survival rate in a dose-dependent manner (1:8, 1:4, 1:2, 1:1) in PDSCs. The cytotoxicity of Nexus RMGIC was inhibited by treatment with 10-mM NAC. In addition, the production of ROS was detected by immunofluorescence after PDSCs were exposed to Nexus RMGIC. However, ROS generation was significantly suppressed in the NAC pretreatment compared with the Nexus RMGIC group. Conclusions: Nexus RMGIC increased the cytotoxicity and ROS generation. ROS was involved in Nexus RMGIC-induced cell toxicity.

Abstract

Objectives: This study aimed to investigate the cytotoxicity of Nexus RMGIC, an indirect restorative cement, on cell survival rate and reactive oxygen species (ROS) production in periodontal stem cells (PDSCs). Methods: PDSCs were incubated with serially diluted Nexus RMGIC eluates with and without the addition of N-acetyl-cysteine (NAC). Cell survival was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The ROS generation was determined by measuring the fluorescence intensity for 2′,7′-dichlorofluorescin diacetate. Results: Nexus RMGIC exposure decreased cell proliferation and cell survival rate in a dose-dependent manner (1:8, 1:4, 1:2, 1:1) in PDSCs. The cytotoxicity of Nexus RMGIC was inhibited by treatment with 10-mM NAC. In addition, the production of ROS was detected by immunofluorescence after PDSCs were exposed to Nexus RMGIC. However, ROS generation was significantly suppressed in the NAC pretreatment compared with the Nexus RMGIC group. Conclusions: Nexus RMGIC increased the cytotoxicity and ROS generation. ROS was involved in Nexus RMGIC-induced cell toxicity.

참고문헌 (32)

  1. B E Kent, "The properties of a glass ionomer cement", British Dental Journal, 135(7), pp.322~326 (1973), Springer Science and Business Media LLC[DOI]
  2. Wilson AD, "Resin-modified glass-ionomer cements", Int J Prosthodont, 3(5), pp.425~429 (1990)
  3. EE Hill, "A clinically focused discussion of luting materials", Australian Dental Journal, 56(Suppl 1), pp.67~76 (2011), Wiley[DOI]
  4. Kirsten L. Van Landuyt, "Systematic review of the chemical composition of contemporary dental adhesives", Biomaterials, 28(26), pp.3757~3785 (2007), Elsevier BV[DOI]
  5. H. Schweikl, "Inhibition of TEGDMA and HEMA-induced genotoxicity and cell cycle arrest by N-acetylcysteine", Dental Materials, 23(6), pp.688~695 (2007), Elsevier BV[DOI]
  6. M. Gallorini, "HEMA-induced cytotoxicity: oxidative stress, genotoxicity and apoptosis", International Endodontic Journal, 47(9), pp.813~818 (2014), Wiley[DOI]
  7. S. A. Mazzaoui, "Long-term quantification of the release of monomers from dental resin composites and a resin-modified glass ionomer cement", Journal of Biomedical Materials Research, 63(3), pp.299~305 (2002), Wiley[DOI]
  8. J. Durner, "The toxicokinetics and distribution of 2-hydroxyethyl methacrylate in mice", Biomaterials, 30(11), pp.2066~2071 (2009), Elsevier BV[DOI]
  9. K.L. Van Landuyt, "How much do resin-based dental materials release? A meta-analytical approach", Dental Materials, 27(8), pp.723~747 (2011), Elsevier BV[DOI]
  10. W. Geurtsen, "Residual monomer", additive release and variability in cytotoxicity of light-curing glass-ionomer cements and compomers, 1998, pp.77(12), 2012~2019[DOI]
  11. Ji Hyun Kwon, "Inhibition of odontogenic differentiation of human dental pulp cells by dental resin monomers", 생체재료학회지, 19(2), pp.99~105 (2015), 한국생체재료학회[DOI]
  12. Jan T. Samuelsen, "Apoptosis induced by the monomers HEMA and TEGDMA involves formation of ROS and differential activation of the MAP-kinases p38, JNK and ERK", Dental Materials, 23(1), pp.34~39 (2007), Elsevier BV[DOI]
  13. Paula Alejandra Baldion, "Dental resin monomers induce early and potent oxidative damage on human odontoblast-like cells", Chemico-Biological Interactions, 333, pp.109336~ (2021), Elsevier BV[DOI]
  14. Susana Bandarra, "Biocompatibility of self-adhesive resin cement with fibroblast cells", The Journal of Prosthetic Dentistry, 125(4), pp.705.e1~705.e7 (2021), Elsevier BV[DOI]
  15. Anthony T. J. Goon, "Contact allergy to (meth)acrylates in the dental series in southern Sweden: simultaneous positive patch test reaction patterns and possible screening allergens", Contact Dermatitis, 55(4), pp.219~226 (2006), Wiley[DOI]
  16. Martin Havmose, "Contact allergy to 2‐hydroxyethyl methacrylate in Denmark", Contact Dermatitis, 82(4), pp.229~231 (2020), Wiley[DOI]
  17. Lena Stanislawski, "TEGDMA-induced toxicity in human fibroblasts is associated with early and drastic glutathione depletion with subsequent production of oxygen reactive species", Journal of Biomedical Materials Research Part A, 66A(3), pp.476~482 (2003), Wiley[DOI]
  18. Dong Hee Lee, "Involvement of oxidative stress in mutagenicity and apoptosis caused by dental resin monomers in cell cultures", Dental Materials, 22(12), pp.1086~1092 (2006), Elsevier BV[DOI]
  19. Else Morisbak, "Cell toxicity of 2-hydroxyethyl methacrylate (HEMA): the role of oxidative stress", European Journal of Oral Sciences, 123(4), pp.282~287 (2015), Wiley[DOI]
  20. Yang Jiao, "N-Acetyl Cysteine Depletes Reactive Oxygen Species and Prevents Dental Monomer-Induced Intrinsic Mitochondrial Apoptosis In Vitro in Human Dental Pulp Cells", PLOS ONE, 11(1), pp.e0147858~ (2016), Public Library of Science (PLoS)[DOI]
  21. "ISO 7405. Dentistry - Evaluation of biocompatibility of medical devices used in dentistry" (2018)[URL]
  22. "ISO 10993-5. Biological evaluation of medical devices - part 5: Tests for in vitro cytotoxicity" (2009)[URL]
  23. "The prevalence of periodontal disease"[URL]
  24. Govinda Bhattarai, "Resveratrol prevents alveolar bone loss in an experimental rat model of periodontitis", Acta Biomaterialia, 29, pp.398~408 (2016), Elsevier BV[DOI]
  25. Tae-Il Kim, "Analysis of histone deacetylase inhibitor-induced responses in human periodontal ligament fibroblasts", Biotechnology Letters, 35(1), pp.129~133 (2013), Springer Science and Business Media LLC[DOI]
  26. Fábio Sá Carneiro Sczepanik, "Periodontitis is an inflammatory disease of oxidative stress: We should treat it that way", Periodontology 2000, 84(1), pp.45~68 (2020), Wiley[DOI]
  27. Zewen Liu, "Role of ROS and Nutritional Antioxidants in Human Diseases", Frontiers in Physiology, 9(9), pp.477~ (2018), Frontiers Media SA[DOI]
  28. Maureen Redza-Dutordoir, "Activation of apoptosis signalling pathways by reactive oxygen species", Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1863(12), pp.2977~2992 (2016), Elsevier BV[DOI]
  29. Takeshi Tsubata, "Involvement of Reactive Oxygen Species (ROS) in BCR Signaling as a Second Messenger", Advances in Experimental Medicine and Biology, 1254, pp.37~46 (2020), Springer Singapore[DOI]
  30. Manasa Ambati, "Evaluation of oxidative stress in chronic periodontitis patients following systemic antioxidant supplementation: A clinical and biochemical study", Journal of Natural Science, Biology and Medicine, 8(1), pp.99~103 (2017), EManuscript Technologies[DOI]
  31. Rung-Shu Chen, "The effects of low-dose 2-hydroxyethyl methacrylate on apoptosis and survival in human dental pulp cells", Journal of the Formosan Medical Association, 120(6), pp.1332~1339 (2021), Elsevier BV[DOI]
  32. H. Schweikl, "Critical role of superoxide anions and hydroxyl radicals in HEMA-induced apoptosis", Dental Materials, 33(1), pp.110~118 (2017), Elsevier BV[DOI]

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