세륨옥사이드나노입자(Cerium oxide nano particles: CNPs)를 함유한 치면열구전색재의 Streptococcus mutans 부착량 변화
Changes in the amount of adhesion of Streptococcus mutans to pit and fissure sealant incorporating cerium oxide nano particles(CNPs)
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Objectives: The aim of this study was to investigated the surface roughness and change in the amount of adhesion of Streptococcus mutans to the commercial pit and fissure sealant containing cerium oxide nano particles(CNPs). Methods: The CNPs was incorporated into a commercial pit and fissure sealant at 0-4.0 wt%. Disk Specimens (φ 10 mm × 2 mm) were prepared by light polymerization the front and back for 40s. Average surface roughness was measured and Streptococcus mutans adhesion was observed under confocal laser scanning microscopy (CLSM) after 24 hour. Data were statistically analyzed by one-way ANOVA and Tukey HSDa post-hoc test. Results: Difference of the surface roughness(Ra) between groups was not statistically significant in both non CNPs group and CNPs group(p>0.05). In CNPs group, the amount of S. mutans adhesion was significantly different between control group and decreased in order of CNPs 4.0, CNPs 0.5, CNPs 1.0 and CNPs 2.0(p<0.05). Conclusions: Within the limitation of this study, these aspects of oral bacteria performances suggest potential usefulness of the CNPs incorporation, especially CNPs 1% and 2%, in pit and fissure sealant for inducing antibacterial effect.
Abstract
Objectives: The aim of this study was to investigated the surface roughness and change in the amount of adhesion of Streptococcus mutans to the commercial pit and fissure sealant containing cerium oxide nano particles(CNPs). Methods: The CNPs was incorporated into a commercial pit and fissure sealant at 0-4.0 wt%. Disk Specimens (φ 10 mm × 2 mm) were prepared by light polymerization the front and back for 40s. Average surface roughness was measured and Streptococcus mutans adhesion was observed under confocal laser scanning microscopy (CLSM) after 24 hour. Data were statistically analyzed by one-way ANOVA and Tukey HSDa post-hoc test. Results: Difference of the surface roughness(Ra) between groups was not statistically significant in both non CNPs group and CNPs group(p>0.05). In CNPs group, the amount of S. mutans adhesion was significantly different between control group and decreased in order of CNPs 4.0, CNPs 0.5, CNPs 1.0 and CNPs 2.0(p<0.05). Conclusions: Within the limitation of this study, these aspects of oral bacteria performances suggest potential usefulness of the CNPs incorporation, especially CNPs 1% and 2%, in pit and fissure sealant for inducing antibacterial effect.
참고문헌 (31)
- 박경화, "우리나라 다문화가족과 일반가족 중․ 고등학생의 구강질환증상 비교", 한국치위생학회지, 17(2), pp.193~202 (2017), 한국치위생학회[DOI]
- 강재민, "치면열구전색재와 충전재료에 대한 우식유발 세균의 부착특성 비교", 대한치과재료학회지, 42(3), pp.229~238 (2015), 대한치과재료학회[DOI]
- 전수경, "생체활성 유리 나노입자 첨가량에 따른 치면열구전색제의 물성평가와 세균부착 억제 효과", 한국콘텐츠학회 논문지, 18(3), pp.542~549 (2018), 한국콘텐츠학회[DOI]
- Kim JW, "An experimental study on the anticariogenic effect of fluoride-releasing pit and fissure sealant", J Korean Acad Pediatr Dent, 25(4), pp.849~857 (1998)
- Jung YB, "A comparative experimental study on hardness in several materials for sealants", J Korean Acad Oral Health, 20(2), pp.247~257 (1996)
- Jung HK, "Pit and fissure sealant", The Journal Korean Dent Assoc, 26(5), pp.384~388 (1998)
- Cuto EI, "Sealing of pits and fissures with an adhesive resin:Its use in caries prevention", J Am Dent Assoc, 21(1), pp.121~128 (1967)[DOI]
- 남상미, "Q-Ray View를 이용한 치면열구전색재의 유지상태 평가", 대한치과의사협회지, 58(3), pp.140~151 (2020), 대한치과의사협회[DOI]
- 윤현서, "Streptococcus mutans와 Porphyromonas gingivalis에 대한 Basil Oil의 항균효과", 대한통합의학회지, 6(3), pp.131~139 (2018), 대한통합의학회
- Järvinen H, "In vitro susceptibility of streptococcus mutans to chlorhexidine and six other antimicrobial agents", Antimicrob Agents Chemother, 37(5), pp.1158~1159 (1993)
- Ribeiro J, "In vitro antibacterial effect of chlorhexidine added to glass-ionomer cements", Eur J Oral Sci, 99(6), pp.533~540 (1991)[DOI]
- Shimazu K, "Evaluation of the ion-releasing and recharging abilities of a resin-based fissure sealant containing S-PRG filler", Dent Mater J, 30(6), pp.923~927 (2011)[DOI]
- Pai MR, "Evaluation of antiplaque activity of Azadirachta indica leaf extract gel-a 6-week clinical study", J Ethnopharmacol, 90(1), pp.99~103 (2004)[DOI]
- Masadeh MM, "Cerium oxide and iron oxide nanoparticles abolish the antibacterial activity of ciprofloxacin against gram positive and gram negative biofilm bacteria", Cytotechnology, 67(3), pp.427~435 (2015)[DOI]
- Wu W, "Preparation of new sunscreen materials Ce1−xZn xO2−x via solid-state reaction at room temperature and study on their properties", Rare Metals, 29(2), pp.149~153 (2010)[DOI]
- Kim YH, "Variety of properties and functions of cerium", KISTI Analysis report, 4, pp.1~5 (2010)
- Santos C, "Antimicrobial activity of nano cerium oxide(IV)(CeO2)against Streptococcus mutans", BMC Proc, 8(Suppl 4), pp.P48~ (2014)
- Thakur N, "Synthesis and biomedical applications of nanoceria, a redox active nanoparticle", J Nanobiotechnology, 17(1), pp.1~27 (2019)[DOI]
- Xu J, "CeO2 nanocrystals : seed-mediated synthesis and size control", Materials Research Bulletin, 43(4), pp.990~995 (2008)[DOI]
- Ikeda-OA, "Hydrolysis of tetravalent cerium for a simple route to nanocrystalline cerium dioxide : an in situ spectroscopic study of nanocrystal evolution", Chem Eur J, 19(23), pp.7348~7360 (2013)[DOI]
- Jee HJ, "The inhibity effect of light-cured pit & fissure sealant incorporating antibacterial filler against s. mutans", Master, pp.2004
- Fard JK, "A review of molecular mechanisms involved in toxicity of nanoparticles", Adv Pharm Bull, 5(4), pp.447~454 (2015)[DOI]
- Hannig M, "Nanomaterials in preventive dentistry", Nat Nanotechnol, 5(8), pp.565~569 (2010)[DOI]
- 윤기영, "박테리아에 대한 나노 입자의 항균 특성 평가", 한국실내환경학회지, 2(1), pp.46~53 (2005), 한국실내환경학회
- Venkatesh KS, "Plant pathogenic fungus F solani mediated biosynthesis of nanoceria : antibacterial and antibiofilm activity", RSC Adv, 6(48), pp.42720~42729 (2016)
- Cuahtecontzi-Delint R, "Enhanced antibacterial activity of CeO2 nanoparticles by surfactants", Int J Chem React Eng, 11(2), pp.781~785 (2013)
- Kim YJ, "Plaque adhesion on the surface of various composite resin", J Koran Acad Pediatr Dent, 31(4), pp.547~554 (2004)
- 정미애, "저분자 키토산이 치과용시멘트의 표면특성과 구강세균 부착에 미치는 영향", 한국콘텐츠학회 논문지, 19(2), pp.277~283 (2019), 한국콘텐츠학회[DOI]
- Arumugam A, "Synthesis of cerium oxide nanoparticles using Gloriosa superba L leaf extract and their structural, optical and antibacterial properties", Mater Sci Eng C, 49, pp.408~415 (2015)[DOI]
- Magdalane CM, "Photocatalytic activity of binary metal oxide nanocomposites of CeO2", CdO nanospheres : investigation of optical and antimicrobial activity, 2016, pp.163, 77~86
- Masadeh MM, "Cerium oxide and iron oxide nanoparticles abolish the antibacterial activity of ciprofloxacin against gram positive and gram negative biofilm bacteria", Cytotechnology, 67(3), pp.427~435 (2015)[DOI]
