BacT/Alert3D 장비를 이용한 혈액배양에서 FAN-A 호기성 배양병의 성능 평가
Evaluation of FAN-aerobic Blood Culture Bottle in BacT/Alert3D System
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초록
배경：BacT/Alert3D System (bioMerieux, Durham, NC, USA)을 이용한 혈액배양에서 FAN-A 호기성 배양병의 혈액배양 성적이 STD-A 호기성 배양병보다 더 좋은지를 확인할 필요가 있었다. 방법：2004년 7-12월 중 부산대학교병원 응급실에서 혈액배양이 의뢰된 것 중 일부인 596쌍의 배양에서 FAN-A 호기성 배양병을 추가로 접종하여 배양을 실시하고 STD-A의 배양성적과 비교하였다. 결과：596쌍의 배양에서 혈액배양의 양성률은 84/596(14.1%)이었다. 그 중 STD-A에만 자란 경우 2.5%, FAN-A에만 자란 경우 5.9%, 양쪽에 모두 자란 경우 5.7%로, FAN-A의 양성률(11.6%)이 STD-A의 양성률(8.2%)보다 유의하게 높았다(p<0.001). 검출 균종 및 검출 시간에는 차이가 없었다. 결론：BacT/Alert3D를 이용한 혈액배양에서 FAN-A 배양병을 사용하면 STD-A 배양병을 사용한 것보다 배양 양성률을 더 높일 수 있을 것으로 생각한다.
Abstract
Background: This study was designed to evaluate the performance of FAN-aerobic bottles (FANA) in comparison with standard-aerobic bottles (STD-A) in BacT/Alert3D blood culture system. Methods: A total of 596 pairs of blood cultures, submitted from Emergency Department of Pusan National University Hospital between July and December 2004, were evaluated. In addition to the routine blood culture protocol using standard blood culture bottles, 5 ml of blood samples was inoculated into FAN-A bottles for this study. Results: Microorganisms were grown in 84 (14.1%) of 596 cultures; of those, 15 were positive in STD-A only (2.5%), 35 in FAN-A only (5.9%), and 34 in both (5.7%). The positive rate in FAN-A bottles was significantly higher than that in STD-A bottles ( P<0.001). The species of isolates and detection time showed no difference between the blood culture bottles. Conclusion: In the BacT/Alert3D blood culture system, the use of FAN-A bottles instead of the standard aerobic bottles should yield a higher positive rate.
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