Risk of inaccurate species identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and of false carbapenem resistance by automated susceptibility analysis of Enterobacter spp.
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Enterobacter species were isolated from the sputum of an 84-year-old female patient withfever, chills, cellulitis in the right elbow, and pyogenic arthritis in the left shoulder. Thebacteria were identified using matrix-assisted laser desorption/ionization time-of-flight massspectrometry (MALDI-TOF MS) and whole-genome sequencing (WGS). This strain was definedas a carbapenem-resistant Enterobacterales (CRE) by the MicroScan NG MIC 44 panel withoutapplying the revised guidelines of the Korea Disease Control and Prevention Agency properly. MALDI-TOF MS is commonly used to determine species, but the accurate identification ofEnterobacter subspecies remains challenging. An Etest or disk diffusion test is recommendedto confirm ertapenem resistance when an isolate exhibits resistance solely to ertapenemamong carbapenems
Abstract
Enterobacter species were isolated from the sputum of an 84-year-old female patient withfever, chills, cellulitis in the right elbow, and pyogenic arthritis in the left shoulder. Thebacteria were identified using matrix-assisted laser desorption/ionization time-of-flight massspectrometry (MALDI-TOF MS) and whole-genome sequencing (WGS). This strain was definedas a carbapenem-resistant Enterobacterales (CRE) by the MicroScan NG MIC 44 panel withoutapplying the revised guidelines of the Korea Disease Control and Prevention Agency properly. MALDI-TOF MS is commonly used to determine species, but the accurate identification ofEnterobacter subspecies remains challenging. An Etest or disk diffusion test is recommendedto confirm ertapenem resistance when an isolate exhibits resistance solely to ertapenemamong carbapenems
참고문헌 (12)
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