본문 바로가기
KERD
논문KCI 등재

브루셀라병 혈청검사 양성 수소와 12개월령 이하 소에서 균의 분리 및 동정

Isolation Brucella spp from sero-positive bulls and calves below twelve months old

이희영|이종진|이용창|남향미|류재윤|변정운|송영강
한국동물위생학회 (THE KOREAN SOCIETY OF VETERINARY SERVICE)|2007.09|한국동물위생학회지|제30권 제3호|pp. 375-384

1

조회

0

다운로드

3

인용

0

참고문헌

주제분류수의학

초록

Abstract

This study was conducted to investigate the characteristics of brucellosis in Korean native cattle in a farm where bovine brucellosis was confirmed 3 times from September 2006 to March 2007. Of 74 bulls serum samples examined, 21(28.4%) were positive by Rose-Bengal test(RBT) and Standard tube agglutination test(STAT). In the isolation test from seropositive bulls, B abortus was isolated and identified from 2 specimens (testis, intestinal lymph node) among 6 kinds of specimens including blood, urine, feces and soil. Isolation rate of intestinal lymph node and testis was 25%(3/12 cases)and 16.7%(2/12), respectively. B abortus was also isolated from calves below 12 months old, i.e., 1 isolate(25.0%) was confirmed from testis, 4(40.0%) from supramammary lymph nodes and 1(25.0%) from intestinal lymph node. All isolates had Brucella specific 16s rRNA with 905bp band detected byPCR assay. For the more effective control of bovine brucellosis in korea, this paper would like to suggest that all of bulls and calves should be included in the screening tests.

참고문헌 (27)

  1. Forbes LB, "mission of brucellosis from reindeer to cattle. JAVMA 203", pp.294~
  2. Forbes LB, "Infection of cattle with Brucella abortus biovar 1 isolated from a bison in Wood Buffalo National Park", 37(7), pp.415~419 (1996)
  3. "Kosilov LA. 1993. A case of human infection with brucellosis from a cat. AH Mikrobiol Epidemiol Immunobiol 4:66", pp.68~
  4. "Lopez-Merino A. 1991. Brucellosis. Advance in perspectives. Publication Tcnica del INDRE-SSA 6:1", pp.54~
  5. Kim ST, "Brucellosis outbreak of Korean indi?genous cattle at Yeongwol and Pyeong?chang country Korea", 28(4), pp.387~392 (2005)
  6. Rijpens NP, "Direct detection of Brucella spp. in raw milk by PCR and reverse hybridization with 16S-23S RNA spacer probes", 62, pp.683~1688 (1996)
  7. Leal-Klevezas DS, "Single-step PCR for detection of Brucella spp from blood and milk of infected animals", 33, pp.3087~3090 (1995)
  8. Jung SC, "Development of PCR assay for the detec?tion of Brucella spp in bovine serum", 38(2), pp.345~352 (1998)
  9. Corbel MJ, "173AL. In: Bergey′s Manual of Systematic Bacteriology"
  10. 심항섭, "경기도에서 발생하는 유우 부루셀라병에 관한 연구", 19(3), pp.189~198 (1996)
  11. 박노찬, "경북지방의 부루셀라병에 관한 연구", 21(4), pp.451~465 (1998)
  12. Timoney JF, "logy and infectious disease of domestic animals. 8 eds. Comstock Publishing Associate", Ithaca and London:127-138.
  13. Bae J, "Generation of baculovirus-Brucella abortus heat shock protein recombinants; Mouse immune responses against the recombinants, and B. abortus superoxide dismutase and L7", L12 recom?binant proteins, 1999, ~[DOI]
  14. Yagupsky P, "Detection of Brucella in blood cultures", 37, pp.3437~3442 (1999)
  15. Perry MB, "Bundle DR. 1990. Antigenic relationships of the lipopolysaccharides of Escherichia hermannii strains with those of Escherichia coli 0157:H7", pp.581391~1395
  16. "Tizard IR. 1980. Studies on the relationship between Yersinia enterocolitica IX and Brucella abortus agglutinins in naturally infected animals. Res Vet Sci 28:311-314."
  17. "Alton GG. 1975. Jones LM. Laboratory techniques in brucellosis. World Health Organization", pp.~163
  18. Alonso- Urme, "et al. 1994. Evaluation of sero logical tests for diagnosis of Brucella melitensis infection of goats. J Clin Microbiol 32:1159-1165.", Marin C
  19. Leal-Klevezas DS, "Single step PCR for the detection of Brucella spp from blood and milk of infected animals", 33, pp.3087~3090 (1995)
  20. Leal-Klevezas DS, "Epidemio?logical molecular de un foco primario de brucelosis en el Estado de Maxico", 16, pp.149~153 (1999)
  21. Fekete A, "1992. Detection of Brucella by polymerase chain reaction in bovine fetal and maternal tissues. J Vet Diagn Invest 4:79-83."
  22. Fekete A, "et al.. 1990. Preliminary development of a diag nostic tests for Brucella using polymerase chain reaction. J Appl Bacteriol 69:216", pp.227~
  23. Herman L, "fication of Brucella spp. by Using the polymerase chain reaction. App Env Microbiol 58:2099-2101."
  24. Leal-Klevezas DS, "Use of poly?merase chain reaction to detect Brucella abortus biovar 1 in infected goats", 75, pp.91~97
  25. Fekete A, "sensitive detection of Bru cella abortus by polymerase chain reaction without extraction of DNA. Biotech Tech 4:31-34."
  26. Brown DD, "et al. 1975. Brucellosis: a study of five calves from reactor dams. Vet Rec 97:52-54."
  27. "Nicoletti P 1980. The epidemiology of bovine Brucellosis. Adv Vet Sci Comp Med 24:69-98."

관련 논문AI추천

소 브루셀라병의 혈청학적 진단법 비교실험허진, 백병걸, 이현진, 정재명, Ibulaimu Kakoma한국동물위생학회 · 한국동물위생학회지2007 · 제30권 제3호경북지방 소에서 분리한 Brucella abortus의 생화학적 특성김성국, 김영환, 박청규, 이영주, 조민희한국동물위생학회 · 한국동물위생학회지2009 · 제32권 제2호Bacteriological detection of Brucella abortus and its characterization by PCR in the sporadic outbreak of bovine brucellosis in Gyeonggi province김혜성, 심항섭, 양수정, 우종태, 이성식한국동물위생학회 · 한국동물위생학회지2007 · 제30권 제2호개 혈액 재료에서의 Brucella 검출을 위한 진단방법의 비교허문, 권순오, 박미연, 박상희, 안동춘, 이영주, 한태욱, TRUONGQUANG LAM한국동물위생학회 · 한국동물위생학회지2009 · 제32권 제4호경북지방 개 브루셀라병 다두 발생농장의 혈청학적 및 세균학적 조사김성국, 김순태, 서희진, 장영술, 조민희한국동물위생학회 · 한국동물위생학회지2010 · 제33권 제2호경북지역 애견 번식장에서 분리한 Brucella canis의 생화학적 특성 분석김성국, 김영환, 엄현정, 이양수, 장성준, 조민희, 홍현표한국동물위생학회 · 한국동물위생학회지2007 · 제30권 제3호

이 간행물의 다른 논문 (19건)

Isolation and characterization of Vitreoscilla mutant defective in catalase-peroxidase hydropreroxidase 1김희정, 박기인, 이존화, 문자영pp. 291-304전북 익산지역 양돈장에서 돼지 생식기 호흡기 증후군, 서코바이러스-2 및 마이코프라즈마 폐염으 항체가 조사형상기, 서이원, 이지영, 정병우, 추금숙, 김지영pp. 305-312Avian adenovirus 관련 심낭수종-봉입체간염 증후군 진단에 관한 연구이정원, 송희종, 추금숙pp. 313-319육계에서 봉입체성 간염 및 전염성 F낭병 발생 증례이지영, 이정원, 서재식, 조현웅, 권미순, 추금숙, 송희종pp. 321-327농가 생산성 증대를 위한 육계 초생추 질병실태 조사엄성심, 조범준, 이병종, 이성효, 고원석, 배정준pp. 329-338
원문 보기