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경북지역 애견 번식장에서 분리한 Brucella canis의 생화학적 특성 분석

Biochemical characterization and PFGE pattern of Brucella canis isolated from kennels in Gyeongbuk province

김영환|조민희|이양수|엄현정|홍현표|김성국|장성준
한국동물위생학회 (THE KOREAN SOCIETY OF VETERINARY SERVICE)|2007.09|한국동물위생학회지|제30권 제3호|pp. 363-374

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Abstract

A biochemical characterization and antimicrobial drugs susceptibility study was conducted in four breeding kennel which was canine abortion caused by Brucella canis in Gyeongbuk province in 2003-2006. Total of 267 dogs domesticated in the four kennel were examination. Among them, 143(53.6%) dogs were sero-positive and 25 of blood samples were isolated to Brucella canis. At amplification of 35KDa-BCSP gene using PCR, 711bp DNA fragment was same visible in 25 isolates and B canis RM6/66. Biochemical characterization of B canis isolated was non-hemolytic, no production of H2S, no fermentation of carbohydrates, catalase-positive, oxidase-positive, indol-negative, hydrolyzation of urea, reduction of nitrate and development of thionin dye medium. Using disk-diffusion method, all of 25 strains tested were found to be highly susceptible to tetracycline, aminoglycoside, quinolone, macrolide antibiotics, rifampin and ampicillin in vitro. Using PFGE with restriction enzyme SmiⅠ, 25 isolates tested were typed to 2 pattern, S1 and S2.

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