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개 혈액 재료에서의 Brucella 검출을 위한 진단방법의 비교

Comparison of diagnostic methods for detection of Brucella species in dog blood samples

이영주|박미연|박상희|한태욱|안동춘|허문|권순오|TRUONGQUANG LAM
한국동물위생학회 (THE KOREAN SOCIETY OF VETERINARY SERVICE)|2009.12|한국동물위생학회지|제32권 제4호|pp. 335-341

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참고문헌

주제분류수의학

초록

Abstract

Canine brucellosis produce abortions and infertility in dogs and currently is diagnosed by serological methods such as rapid slide agglutination test with 2-mercaptoethanol (2-ME RSAT) and immunochromatographic assay (ICA). Bacterial isolation is considered gold standard for Brucella diagnosis and the polymerase chain reaction (PCR) is an alternative method to bacterial isolation. A total of 36 whole blood samples were collected from dogs reared in area of Chuncheon and were subjected to serology (2-ME RSAT and ICA for B. canis, Rose Bengal test and C-ELISA for B. abortus), blood culture and 3 types of PCRs (BSCP31, 16s rRNA, and OMP-2). All blood samples were negative by serology and blood cultures. The BCSP31 and the OMP-2 PCR detected 5 samples were positive whereas the 16S rRNA PCR detected all samples were negative as serological methods and blood culture did. From the results observed in the present study, we conclude that 16S rRNA PCR could be used for direct PCR for canine blood samples.

참고문헌 (31)

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