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소와 돼지유래 다제내성 Salmonella속 균의 분자유전학적 특성

Molecular genetic characterization of multiple antimicrobial resistant Salmonella spp. isolated from pigs and cattle

김용환|이동수|이강록|정병열|이우원
한국동물위생학회 (THE KOREAN SOCIETY OF VETERINARY SERVICE)|2009.03|한국동물위생학회지|제32권 제1호|pp. 61-76

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초록

Abstract

At the present study, it was aimed to explore the molecular genetic characterization of multiple antimicrobial resistant Salmonella spp. isolates from pigs and cattle. A total of 138 Salmonella Typhimurium (S. Typhimurium) isolates were typed with phage, among them, 83.3% of S. Typhimurium tested could divide into a 10 phage types. definitive type 103 (DT193) (25.4%) and DT195 (24.6%) were exhibited as the dominant types. DT104 and U302 were found from pigs and cattle. On the other hand, S. Enteritidis had 6 phage types, of them, phage type 21 (PT21) and PT11b were the popular types. In the plasmid profiles, 135 of S. Typhimurium isolates were exhibited 1 to 6 plasmid bands which molecular weight ranged from 90 to 2kb. 35 isolates (25.4%) harbored a 90kb plasmid which is thought to be the serotype specific virulence plasmid. Two of twenty five S. Enteritidis had common plasmids at 2 and 1.5kb. With multiplex polymerase chain reaction, virulence genes (invA and spvC) were detected from all Salmonella spp. from 167 of S. Typhimurium, S. Enteritidis and chloramphenicol resistant S. chwarzengrund, but some drug resistant genes, such as PSE-1, cml/tetR and flo were not determined but other drug resistant genes, for example TEM and int were found. The detection rates of spvC, TEM and int gene was 35.3%, 29.3% and 72.5%, respectively. The TEM gene was highly popular in S. Typhimurium, which was detected from ampicillin and amoxicillin resistant strains as 95.9%. int gene was able to detect from all the isolates identified as multidrug resistsnt (MDR), particularly DT193 was thought as the most prevalent virulence and multidrug resistance isolate. The major plasmid profile and drug resistance pattern of DT193 were 90, 40, 10.5, 6.3, 3.0kb and ACCbDNaPSSuT, respectively. MDR was commonly found in other phage types, particularly DT104, U302 and DT203.

참고문헌 (49)

  1. 김상윤, "경북지역 가축에서 분리된 Salmonella 속균의 역학적 특성 및 병원성", 박사, pp.2000
  2. 김원용, "가금에서 분리한 Salmonella 속균의 항균물질에 대한 감수성 및 plasmid profile", 대한수의학회지, 35(3), pp.537~542 (1995)
  3. 우용구, "한국의 동물과 사람에서 분리한 Salmonella enteritidis의 Phage types", 대한수의학회지, 40(3), pp.515~524 (2000)
  4. 정대영, "전남지역 도축장에서 분리된 Salmonella typhimurium의 병원성에 관한 연구", 한국가축위생학회지, 26(1), pp.39~50
  5. 최원필, "동물에서 분리된 Salmonella균의 병원성관련 Plasmid에 관한 연구", 대한수의학회지, 32(3), pp.369~376 (1992)
  6. Anderson DM, "Plasmid studies of Salmonella typhimurium phage type 179 resistant to ampicillin, tetracycline,sulfonamides and trimethoprim", J Hyg Camb, 85, pp.293~300 (1980)
  7. Anderson ES, "Bacteriophagetyping designations of Salmonella typhimurium", J Hyg, 78, pp.297~300 (1977)
  8. Ang-Kucuker M, "Phage types,antibiotic susceptibilities and plasmid profiles of Salmonella typhimurium and Salmonella enteritidis strains isolated in Istanbul, Turkey", Clin Microbiol Inpect, 6(11), pp.593~599 (2000)
  9. Baggesen DL, "Cahracterization of Salmonella enterica serovar Typhimurium DT104 isolated from Denmark and comparison with isolates from Europe and the United States", J Clin Microbiol, 38(4), pp.1581~1586 (2000)
  10. Betancor L, "Random amplified polymorphic DNA and phenotyping analysis of Salmonella enterica serovar Enteritidis isolates collected from humans and poultry in Urugury from 1995 to 2002", J Clin Microbiol, 42(3), pp.1155~1162 (2004)
  11. Bolton LF, "Detection of multidrug-resistant Salmonella enterica serotype Typhimurium DT104 based on a gene which confers cross-resistance to florfenicol and chloramphenicol", J Clin Microbiol, 37(5), pp.1348~1351 (1999)
  12. Briggs CE, "Molecular characterization of an antibiotic resistance gene cluster of Salmonella typhimurium DT104.Antimicrob Agents Chemother 43(4):846-849", Antimicrob Agents Chemother, 43(4), pp.846~849 (1999)
  13. Carlson SA, "Detection of multiresistant Salmonella typhimurium DT104 by multiplex and fluoregenic PCR", Mol Cell Probes, 13, pp.213~222 (1999)
  14. Carlson SA, "Identification of diminished tissue culture invasiveness among multiple antibiotic resistant Salmonella typhimurium", Microb Pathog, 28(1), pp.37~44 (2000)
  15. Chen S, "Characterization of multiple-antimicrobial-resistant Salmonella serovars isolated from reptail meats", Appl Environ Microbiol, 70(1), pp.1~7 (2004)
  16. Chiu CH, "Rapid identification of Salmonella serovars in feces by specific detection of virulence genes, invA and spvC, by an enrichment broth culturemultiplex PCR combination assay", J Clin Microbiol, 34(10), pp.2619~2622 (1996)
  17. Cieslik A, "Phage types, plasmid profiles and chromosomal restriction profiles of Salmonella enterica subsp. enterica ser. Enteritidis (Salmonella enteritidis) isolated in Poland in 1999-2000", Med Dosw Mikrobiol, 53(2), pp.185~196 (2001)
  18. Davies RH, "National survey for Salmonella in pigs, cattle and sheep at slaughter in Great Britain (1999-2000)", J Appl Microbiol, 96(4), pp.750~760 (2004)
  19. Duijkeren EV, "Serotype and phage type distribution of Salmonella strains isolated from humans, cattle, pigs, and chickens in the Netherlands from 1984 to 2001", J Clin Microbiol, 40(11), pp.3980~3985 (2002)
  20. Duijkeren EV, "Antimicrobial susceptibilities of Salmonella srains isolated from humans, cattle, pigs, and chickens in the Netherlands from 1984 to 2001", J Clin Microbiol, 41(8), pp.3574~3578 (2003)
  21. Gahring LC, "Invasion and replication of Salmonella typhimurium in animal cells", Infect Immun, 58, pp.443~448 (1990)
  22. Gebreyes WA, "Molecular characterization of multidrug-resistant Salmonella enterica subsp.enterica serovar Typhimurium isolates from swine", J Clin Microbiol, 40(8), pp.2813~2822 (2002)
  23. Gebreyes WA, "Characterization of antimicrobial- resistant phenotypes and genotypes among Salmonella enterica recovered from pigs on farms, from transport trucks, and from pigs after slaughter", J Food Prot, 67(4), pp.698~705 (2004)
  24. Gebreyes WA, "Trends in antimicrobial resistance, phage types and integrons among Salmonella serotypes from pigs, 1997-2000", J Antimicrob Chemother, 53(6), pp.997~1003 (2004)
  25. Gorman R, "Characterization Salmonella enterica serotype Typhimurium isolates from human,food, and animal sources in the Republic of Ireland", J Clin Microbiol, 42(5), pp.2314~2316 (2004)
  26. Haneda T, "Complete DNA sequence and comparative analysis of the 50-kilobase virulence plasmid of Salmonella enterica serovar Choleraesuis", Infect Immun, 69(4), pp.2612~2620 (2001)
  27. Izumiya H, "Characterization of multidrug-resistant Salmonella enterica serovar Typhimurium isolated in Japan", J Clin Microbiol, 39(7), pp.2700~2703 (2001)
  28. Jelesic Z, "Analysis of the plasmid profile of various Salmonella serotypes", Med Pregl, 53(11), pp.564~567 (2000)
  29. Khan AA, "Detection of multidrug-resistant Salmonella typhimurium DT104 by multiplex polymerase chain reaction", FEMS Microbiol Lett, 182, pp.355~360 (2000)
  30. Kowalczyk-Pecka D, "Antimicrbial susceptibility and phage types of Salmonella enteritidis strains isolated in the Lublin area", Przegl Epidemiol, 57(1), pp.201~209 (2003)
  31. Llanes C, "Propagation of TEM- and PSE-type β-lactamase among amoxicillinresistant Salmonella spp. isolated in France", Antimicrob Agent Chemother, 43(10), pp.2430~2436 (1999)
  32. Majtanova L, "Occurrence of phage types of non-typhoid Salmonella erovar in the Slovak Republic 2000-2003", Epidemiol Mikrobiol lmunol, 53(2), pp.70~73 (2004)
  33. Mare L, "Phage types of Salmonella enterica serovar Enteritidis isolated in South Africa from 1991-1995", Onderstepoort J Vet Res, 67(2), pp.129~133 (2000)
  34. Mayer LW, "Use of plasmid profiles in epidemiologic surveillance of disease outbreaks and intracing the transmission of antibiotic resistance", Clin Microbiol Rev, 1, pp.228~243 (1988)
  35. Millemann Y, "Value of plasmid profiling, ribotyping, and detection of IS200 for tracing avian isolates of Salmonella typhimurium and Salmonella enteritidis", J Clin Microbiol, 33(1), pp.173~179 (1995)
  36. Murray PR, "Manual of clinical microbiology", pp.467~471 (1999), ASM Press
  37. Nastasi, "Surveillance of multidrug-resistant strains of Salmonella enterica serotype Typhimuriumin southern Italy in the years 1992-1997", Eur J Epidemiol, 16(2), pp.135~139 (2000)
  38. Ng LK, "Genetic characterization of antimicrobial resistance in Canadian isolates of Salmonella serovar Typhimurium DT104.Antimicrob", Agents Chemother, 43(12), pp.3018~3021 (1999)
  39. Nygard K, "Emergence of new Salmonella Enteritidis phage types in Europe Surveillance of infections in returning travellers", BMC Med, 2, pp.32~ (2004)
  40. Randall LP, "Antibiotic resistance genes, integrons and multiple antibiotic resistance in thirty-five serotypes of Salmonella enterica isolated from humans and animals in the UK", J Antimicrob Chemother, 53, pp.208~216 (2004)
  41. Rychlik I, "Subdivision of Salmonella enterica serovar Enteritidis phage types PT14b and PT21 by plasmid profiling", Vet Microbiol, 74(3), pp.217~225 (2000)
  42. Sambrook J, "Molecular cloning a laboratory manual", pp.1.21~1.51 (1989), SCH Laboratory Press
  43. Smith-Palmer A, "Epidemiology of Salmonella enterica serovars Enteri-tidis and Typhimurium in animals and people in Scot-land between 1990 and 2001", Vet Rec, 153(17), pp.517~520 (2003)
  44. Swamy SC, "Virulence determinants invA and spvC in Salmonella isolated from poultry products, wastewater, and human sources", Appl Environ Microbiol, 62(10), pp.3768~3771 (1996)
  45. Ozlem Tansel, "A Food-Borne Outbreak Caused by Salmonella Enteritidis", Yonsei Medical Journal, 44(2), pp.198~202 (2003), 연세대학교의과대학
  46. Threlfall EJ, "A comparison of multiple drug resistance in Salmonella from humans and food animals in England and Wales, 1981 and 1990", Epidemiol Infect, 111, pp.189~197 (1993)
  47. Threlfall EJ, "A comparison of antimicrobial susceptibilities in nontyphoidal Salmonella from humans and food animals in England and Wales in 2000", Microb Drug Resist, 9(2), pp.183~189 (2003)
  48. Ward LR, "A phage typing scheme for S enteritidis", Epidemiol Infect, 99, pp.291~294 (1987)
  49. Yang SJ, "Multidrug-resistant Salmonella typhimurium and Salmonella enteritidis identified by multiplex PCR from animals", J Vet Sci, 2(3), pp.181~188 (2001)

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