소와 돼지유래 살모넬라속 균의 약제내성유전자의 특성에 관한 연구
Investigation on antimicrobial resistance genes of Salmonella spp. isolated from pigs and cattle
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Abstract
At the present study, it was aimed to detect virulence genes and antimicrobial resistance genes among 102 strains of 12 Salmonella serotypes isolated from pigs and cattle. In polymerase chain reaction (PCR), invA was detected from all strains of Salmonella spp., spvC was detected from Salmonella enterica serotype Enteritidis (S. Enteritidis) (100%), S. Bradenburg (75%), and S. Typhimurium (20.4%). Drug resistance related genes of 12 types were detected from all strains. TEM (blaTEM) gene was detected from 51 (92.7%) of 55 β-lactams (54 ampicillin or 1 amoxicillin) resistance strains. 55 (100%) of 55 chloramphenicol resistance strains, 3 (100%) of 3 gentamicin resistance strains and 5 (100%) of 5 kanamycin resistance strains did contain cml, aadB, and aphA1-Iab, respectively. strB (89.9%), strA (88.4%), aadA2 (84.1%) and aadA1 (72.5%) were detected from 69 streptomycin resistance strains. sulII and dhfrXII were detected from 49 (100%) of 49 sulfamethoxazole/trimethoprim resistance strains, but sulI was not detected. tetA (97.9%) and tetB (21.6%) were detected from 97 tetracycline resistance strains. int gene was detected from 58 (56.9%) of 102 strains. 54 S. Typhimurium of 102 Salmonella spp. were attempted to detect drug resistance genes. TEM was detected from 44 (95.7%) of 46 β-lactams (45 ampicillin or 1 amoxicillin) resistance strains. cmlA was detected from 51 (100%) of 51 chloramphenicol resistance strains. aadA2 (100%), strA (100%), strB (100%), and aadA1 (79.6%) were detected from 54 streptomycin resistance strains. sul II (100%) and dhfrXII (100%) were detected from 49 sulfamethoxazole/trimethoprim resistance strains. tetA was detected from 54 (100%) of 54 tetracycline resistance strains. int gene was detected from 54 (100%) of 54 strains. The major drug resistance pattern and resistance gene profile were ampicillin, chloramphenicol, streptomycin, sulfamethoxazole/trimethoprim and tetracycline (ACSSuT) and TEM, cmlA, aadA1, aadA2, strA, strB, sulII, dhfrXII, tetA and int, respectively.
참고문헌 (32)
- 김상윤, "경북지역 가축에서 분리된 Salmonella속 균의 역학적 특성 및 병원성", 박사, pp.2000
- 이우원, "소와 돼지유래 Salmonella속 균의 혈청형 및 약제감수성", 한국가축위생학회지, 32(1), pp.49~59 (2009), 한국가축위생학회
- 이우원, "소와 돼지유래 다제내성 Salmonella속 균의 분자유전학적 특성", 한국가축위생학회지, 32(1), pp.61~76 (2009), 한국가축위생학회
- 정대영, "전남지역 도축장에서 분리된 Salmonella typhimurium의 병원성에 관한 연구", 한국가축위생학회지, 26(1), pp.39~50 (2003)
- Arcangioli MA, "Evolution of choloramphenicol resistance, with emergence of cross-resistance to florfenicol, in bovine Salmonella Typhimurium strains implicates definitive phage type (DT) 104", J Med Microbiol, 49(1), pp.103~110 (2000)
- Baggesen DL, "Cahracterization of Salmonella enterica serovar Typhimurium DT104 isolated from Denmark and comparison with isolates from Europe and the United States", J Clin Microbiol, 38(4), pp.1581~1586 (2000)
- Bauer AW, "Antibiotic susceptibility testing by a standardized single disk method", Am J Clin Pathol, 45(4), pp.493~496 (1966)
- Bolton LF, "Detection of multidrug- resistant Salmonella enterica serotype Typhimurium DT104 based on a gene which confers cross-resistance to florfenicol and chloramphenicol", J Clin Microbiol, 37(5), pp.1348~1351 (1999)
- Boyd D, "Characterization of variant Salmonella genomic island 1 multidrug resistance regions from serovars Typhimurium DT104 and Agona", Antimicro Agent and Chemother, 46(6), pp.1714~1722 (2002)
- Boyd D, "Complete nucleotide sequence of a 43-kilobase genomic island associated with the serovar Typhimurium DT104 and its identification in phage type DT120 and serovar Agona", J Bacteriol, 183(19), pp.5725~5732 (2001)
- Bryant MC, "Antibiotics and their laboratory control", pp.41~ (1972), Butterworth
- Chen S, "Characterization of multiple-antimicrobial- resistant Salmonella serovars isolated from reptail meats", Appl Environ Microbiol, 70(1), pp.1~7 (2004)
- Doublet B, "The Salmonella genomic island 1 is an integrative mobilizable element", Mol Microbiol, 55(6), pp.1911~1924 (2005)
- Edwards PR, "Salmonellosis", Adv Vet Sci, 11, pp.1~63 (1967)
- Faldynova M, "Evolution of antibiotic resistance in Salmonella enterica serovar Typhimurium strains isolated in the Czech republic between 1984 and 2002", Antimicro Agents Chemother, 47(6), pp.2002~2005 (2003)
- Gahring LC, "Invasion and replication of Salmonella typhimurium in animal cells", Infect Immun, 58(2), pp.443~448 (1990)
- Gebreyes WA, "Molecular characterization of multidrug-resistant Salmonella enterica subsp. enterica serovar Typhimurium isolates from swine", J Clin Microbiol, 40(8), pp.2813~2822 (2002)
- Guerra B, "Multidrug resistance is Mediated by large plasmids carrying a class 1 integron in the emergent Salmonella enterica serotype〔4, 5,12:i:-〕", Antimicrob Agents Chemother, 45(4), pp.1305~1308 (2001)
- Helmuth R, "Epidemiology of virulence associated plasmids and outer membrane protein patterns within seven common Salmonella serotypes", Infect Immun, 48, pp.175~182 (1985)
- Khan AA, "Detection of multidrug-resistant Salmonella typhimurium DT104 by multiplex polymerase chain reaction", FEMS Microbiol Lett, 182(2), pp.355~360 (2000)
- Lee KW, "Emergence of multidrug-resistant Salmonella enterica serovar Typhi in Korea", Antimicrob Agent Chemother, 48(11), pp.4130~4135 (2004)
- Llanes C, "Propagation of TEM- and PSE-type β-lactamase among amoxicillinresistant Salmonella spp. isolated in France", Antimicrob Agent Chemother, 43(10), pp.2430~2436 (1999)
- Meunier D, "Salmonella enterica serotype Typhimurium DT 104 antibiotic resistance genomic island 1 in serotype Paratyphi B", Emerg Infect Dis, 8(4), pp.430~433 (2002)
- National Committee for Clinical Laboratory Standards, "Performance standards for antimicrobial disc susceptibility tests. 6th ed", Approved standard. NCCLS, 18(1), pp.M2~A6 (1988)
- Pezzella C, "Tetracycline and streptomycin resistance genes,transposons, and plasmids in Salmonella enterica isolates from animals in Italy", Antimicrob Agents Chemother, 48(3), pp.903~908 (2004)
- Randall LP, "Antibiotic resistance genes, integrons and multiple antibiotic resistance in thirty-five serotypes of Salmonella enterica isolated from humans and animals in the UK", J Antimicro Chemother, 53(2), pp.208~216 (2004)
- Soto SM, "Antimicrobial resistance in clinical isolates of Salmonella enterica serotype Enteritidis: relationships between mutations conferring quinolone resistance, integrons,plasmids and genetic types", J Antimicro Chemother, 51(5), pp.1287~1291 (2003)
- Swamy SC, "Virulence determinants invA and spvC in Salmonella isolated from poultry products, wastewater, and human sources", Appl and Environ Microbiol, 62(10), pp.3768~3771 (1996)
- Taitt CR, "Detection of Salmonella enterica serovar Typhimurium by using a rapid, array-based immunosensor", Appl Environ Microbiol, 70(1), pp.152~158 (2004)
- Velge P, "Emergence of Salmonella epidemics: The problems related to Salmonella enterica serotype Enteritidis and multiple antibiotic resistance in other major serotypes", Vet Res, 36(3), pp.267~288 (2005)
- Walker Ra, "Variation in clonality and antibiotic-resistance genes among multiresistant Salmonella enterica serotype Typhimurium phage type U302 (MR U302) from humans, animals and foods", Microb Drug Resist, 7(1), pp.13~21 (2001)
- Yang SJ, "Multidrug-resistant Salmonella typhimurium and Salmonella enteritidis identified by multiplex PCR from animals", J Vet Sci, 2(3), pp.181~188 (2001)
