봉독 분석을 위한 전처리 방법 개발 및 이를 이용한 젖소 원유 중의 봉독 잔류물질 조사
Analysis of bee venom residues in milks of dairy cattle using UHPLC with newly developed pre-processing method
2
조회
0
다운로드
6
인용
0
참고문헌
키워드
초록
Bee venom has been used as to prevent and treat bovine mastitis as natural antimicrobial compoundsin some dairy cattle farms in Korea. It is needed to determine the residual of bee venom in milks ofdairy cattle treated with bee venom. Since bee venom is not approved as a raw material for animaldrugs, the preprocessing method to detect bee venom residual in milk and the tolerance limit for itsresidue has not been established yet in Korea. Therefore, the purpose of this study was to developpre-processing method not affecting major component of bee venom for detection of its residue in milksusing ultra-high performance liauid chromatography (UHPLC). In addition, bee venom residue was alsoanalyzed in milk samples of dairy cattle treated for mastitis with bee venom using UHPLC with thedeveloped pre-processing method in this study. As a result, melittin, histamin and phospolipase A2, themajor components of bee venom, were all detected by UHPLC with the pre-processing method developedin this study. The results of this study suggest that the pre-processing method developed in thisstudy can be useful to detect bee venom residue in dairy cattle milk. We also found that no bee venomresidues were detected in milk samples collected from dairy cattle treated with bee venom after 1 and3 days, respectively.
Abstract
Bee venom has been used as to prevent and treat bovine mastitis as natural antimicrobial compoundsin some dairy cattle farms in Korea. It is needed to determine the residual of bee venom in milks ofdairy cattle treated with bee venom. Since bee venom is not approved as a raw material for animaldrugs, the preprocessing method to detect bee venom residual in milk and the tolerance limit for itsresidue has not been established yet in Korea. Therefore, the purpose of this study was to developpre-processing method not affecting major component of bee venom for detection of its residue in milksusing ultra-high performance liauid chromatography (UHPLC). In addition, bee venom residue was alsoanalyzed in milk samples of dairy cattle treated for mastitis with bee venom using UHPLC with thedeveloped pre-processing method in this study. As a result, melittin, histamin and phospolipase A2, themajor components of bee venom, were all detected by UHPLC with the pre-processing method developedin this study. The results of this study suggest that the pre-processing method developed in thisstudy can be useful to detect bee venom residue in dairy cattle milk. We also found that no bee venomresidues were detected in milk samples collected from dairy cattle treated with bee venom after 1 and3 days, respectively.
참고문헌 (28)
- 김문호, "봉독요법과 봉침요법", pp.67~141 (1992), 한국교육기획
- 남향미, "젖소 유방염에 대한 항생제 사용 지침", 대한수의사회지, pp.638~644 (2010)
- 류일선, "젖소의 유방염발생감소를 위한 예방 및 관리대책", pp.230~243 (2010)
- 식품의약품안전평가원, "축수산물 유해물질 분석법 편람" (2014)
- 한상미, "봉독 간이정제법. 특허 제 10-0758814호", ~ (2007)
- Burgat V, "Residues of drugs veterinary use in food", Rev Pract, 41, pp.985~990 (1991)
- Choi SH, "Therapeutic Effect of Piglets with Bacterial Diarrhea by Natural Honeybee (Apis mellifera) Venom", Korean J Vet Clin Med, 16(1), pp.150~154 (1999)
- Curcio-Vonlanthen V, "Molecular parameters in melittin immunogenicity", J Pept Sci, 3(4), pp.267~276 (1997)
- Jung SC, "Establishment of control system of antibiotics for livestocks", National Veterinary Research and Quarantine Service and Korea Food & Drug Administration, pp.8~12 (2006)
- Habermann E, "On the biochemistry of bee venom peptides, melittin and apamin", Biochem Z, 343(2), pp.192~203 (1965)
- Sangmi Han, "Antibacterial Activity of the Honey Bee Venom against Bacterial MastitisPathogens Infecting Dairy Cows", International Journal of Industrial Entomology, 14(2), pp.137~142 (2007), 한국잠사학회
- Han SM, "Effects of bee venom treatment on growth performance of young pigs", Am J Chin Med, 37(2), pp.833~842 (2009)
- Han SM, "Somatic cell count in milk of bee venom treated dairy cows with mastitis", J ApiProduct ApiMed Sci, 1(4), pp.104~109 (2009)
- Han SM, "Effects of honeybee venom supplementation in drinking water on growth performance of broiler chickens", Poult Sci, 89, pp.2396~2400 (2010)
- 한상미, "봉독 처리가 어미소의 번식효율과 송아지의 증체, 질병발생에 미치는 영향", 한국가축위생학회지, 33(3), pp.287~292 (2010), 한국가축위생학회
- Hays VW, "Efficiency and safety of feed additive use of antibacterial drugs in animal production", Can J Anim Sci, 59, pp.447~456 (1979)
- Hur TY, "Antibacterial effect of Caesalpinia sappan extract against masitis pathogens from dairy cows", J Vet Clin, 26(3), pp.286~290 (2006)
- Kim SJ, "Apamin inhibits THP-1-derived macrophage apoptosis via mitochondria-related apoptotic pathway", Exp Mol Pathol, 93, pp.129~134 (2012)
- Kim ST, "Comparison of fatty acid composition of Staphylococcus sp isolated from bovine mastitis milk", Korean J Vet Serv, 20, pp.37~46 (1997)
- 권진욱, "농약 및 동물용의약품으로 사용되는 약제의 잔류허용기준설정 개선 - 축산물 중 cypermethrin의 잔류 사례", 한국환경농학회지, 30(1), pp.89~97 (2011), 한국환경농학회
- Lin JH, "Acupuncture effects on the body's defense systems", Vet Bull, 50, pp.633~640 (1980)
- Lin YC, "Observation of therapeutic effects of acupuncture treatment in 170 cases of infantile diarrhea", J Tradit Chin Med, 7(3), pp.203~204 (1987)
- Lunt DH, "The insect cytochrome oxidase I gene; evolutionary patterns and conserved primers for phylogenetic studies", Inect Mol Biol, 5(3), pp.153~165 (1996)
- Smith GW, "Ability of hematologic and serum biochemical variables to differentiate Gram-negative and Gram-positive mastitis in dairy cows", J. Vet. Intern. Med, 15, pp.394~400 (2001)
- Tamura K, "MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0", Mol. Biol. Evol, 30, pp.2725~2729 (2013)
- Piek T., "Venoms of the Hymenoptera" (1986), Academic press
- Vick JA, "Effects of whole bee venom and its fractions(apamin and melittin)on plasma cortisol levels in the dog", Toxicon, 10(4), pp.377~380 (1972)
- Wu M, "Interraction of the cyclic antimicrobial cationic peptide bactenecin with the outer and cytoplasmic membrane", J Biol chem, 274(1), pp.29~35 (1999)