천연항생제로서 봉독의 기원 및 지표성분 설정
Origin and effective ingredient standards of honeybee venom as natural antibiotic ingredients
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This research was performed in order to investigate the origin, standard compound, and structural and physical properties of honeybee venom which used as natural antibiotic ingredients to animal. We com-pared the nucleotide sequence of mitochondrial cytochrome c oxidase subunit 1 gene (COI) of honey-bees were collected from Gangwon, Gyeonggi, Chungnam, Gyeongbuk, Gyeongnam province and Suwon. As major constituent of honeybee venom, melittin was assayed by liquid chromatography. X-ray, differential scanning calorimetry (DSC) and fourier transform infrared spectroscopy (FT-IR) were utilized to examine the structural and physical properties of honeybee venom. Based on the 627bp se-quence of COI, Apis mellifera ligustica was determinated honeybees collected from all six regions. Melittin content varied from 50.7 to 68.6 and averaged 59.8%. According to XRD analysis, honeybee venom showed regular crystal structure peaks at 2θ=8.5° and 21.5°. DSC showed that the maximum degration temperature of powder was around 230°C. Through FT-IR analysis, we could identify cross-linking by the presence of peptide peak at 1,500∼1,600 cm−1. In conclusion, the origin of honey-bee venom was Apis mellifera ligustica and effective ingredient standards was melittin content varied from 50.7 to 68.6 as natural antibiotic ingredients.
Abstract
This research was performed in order to investigate the origin, standard compound, and structural and physical properties of honeybee venom which used as natural antibiotic ingredients to animal. We com-pared the nucleotide sequence of mitochondrial cytochrome c oxidase subunit 1 gene (COI) of honey-bees were collected from Gangwon, Gyeonggi, Chungnam, Gyeongbuk, Gyeongnam province and Suwon. As major constituent of honeybee venom, melittin was assayed by liquid chromatography. X-ray, differential scanning calorimetry (DSC) and fourier transform infrared spectroscopy (FT-IR) were utilized to examine the structural and physical properties of honeybee venom. Based on the 627bp se-quence of COI, Apis mellifera ligustica was determinated honeybees collected from all six regions. Melittin content varied from 50.7 to 68.6 and averaged 59.8%. According to XRD analysis, honeybee venom showed regular crystal structure peaks at 2θ=8.5° and 21.5°. DSC showed that the maximum degration temperature of powder was around 230°C. Through FT-IR analysis, we could identify cross-linking by the presence of peptide peak at 1,500∼1,600 cm−1. In conclusion, the origin of honey-bee venom was Apis mellifera ligustica and effective ingredient standards was melittin content varied from 50.7 to 68.6 as natural antibiotic ingredients.
참고문헌 (21)
- 김문호, "봉독요법과 봉침요법", pp.67~141 (1992), 한국교육기획
- 한상미, "봉독 간이정제법", ~ (2007)
- Burgat V, "Residues of drugs veterinary use in food", Rev Pract, 41, pp.985~990 (1991)
- Choi SH, "The Therapeutic Effect of Piglets with Bacterial Diarrhea by Natural Honeybee(Apis mellifera)Venom", Korean J Vet Clin Med, 16(1), pp.150~154 (1999)
- Curcio-Vonlanthen V, "Molecular parameters in melittin immunogenicity", J Pept Sci, 3(4), pp.267~276 (1997)
- Jung SC, "Establishment of control system of antibiotics for livestocks", pp.8~12 (2006), National Veterinary Research and Quarantine Service and Korea Food & Drug Administration
- Habermann E, "On the biochemistry of bee venom peptides, melittin and apamin", Biochem Z, 343(2), pp.192~203 (1965)
- Sangmi Han, "Antibacterial Activity of the Honey Bee Venom against Bacterial MastitisPathogens Infecting Dairy Cows", International Journal of Industrial Entomology, 14(2), pp.137~142 (2007), 한국잠사학회
- Han SM, "Effects of bee venom treatment on growth performance of young pigs", Am J Chin Med, 37(2), pp.833~842 (2009)
- Han SM, "Somatic cell count in milk of bee venom treated dairy cows with mastitis", J ApiProduct ApiMed Sci, 1(4), pp.104~109 (2009)
- Han SM, "Effects of honeybee venom supplementation in drinking water on growth performance of broiler chickens", Poult Sci, 89, pp.2396~2400 (2010)
- 한상미, "봉독 처리가 어미소의 번식효율과 송아지의 증체, 질병발생에 미치는 영향", 한국가축위생학회지, 33(3), pp.287~292 (2010), 한국가축위생학회
- Hays VW, "Efficiency and safety of feed additive use of antibacterial drugs in animal production", Can J Anim Sci, 59, pp.447~456 (1979)
- Kim SJ, "Apamin inhibits THP-1-derived macrophage apoptosis via mitochondria-related apoptotic pathway", Exp Mol Pathol, 93, pp.129~134 (2012)
- Lin JH, "Acupuncture effects on the body's defense systems", Vet Bull, 50, pp.633~640 (1980)
- Lin YC, "Observation of therapeutic effects of acupuncture treatment in 170 cases of infantile diarrhea", J Tradit Chin Med, 7(3), pp.203~204 (1987)
- Lunt DH, "The insect cytochrome oxidase I gene; evolutionary patterns and conserved primers for phylogenetic studies", Inect Mol Biol, 5(3), pp.153~165 (1996)
- Tamura K, "MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0", Mol. Biol. Evol, 30, pp.2725~2729 (2013)
- Piek T., "Venoms of the Hymenoptera" (1986), Academic press
- Vick JA, "Effects of whole bee venom and its fractions(apamin and melittin)on plasma cortisol levels in the dog", Toxicon, 10(4), pp.377~380 (1972)
- Wu M, "Interraction of the cyclic antimicrobial cationic peptide bactenecin with the outer and cytoplasmic membrane", J Biol chem, 274(1), pp.29~35 (1999)