정제봉독의 MAC-T 세포에서 유단백 합성 촉진효과
Stimulation of the milk protein production in MAC-T cells by purified bee venom
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Purified bee venom was collected from colonies of honeybees (Apis mellifera L.) using a bee venom collector under sterile conditions and then purified under strict laboratory conditions. Purified bee venom contained 63.9±5.4% melittin, 10.9±1.6% phospholipase A2, and 2.3±0.3% apamin. Purified bee venom has various anti-bacterial, anti-inflammatory and immunostimulating effects. In this study, we evaluated purified bee venom which are mammary gland cells, MAC-T cells are used to increase the synthesis of milk protein. Purified bee venom promoted the proliferation of MAC-T cells at concentrations below 1 g/mL, but cytotoxicity at 10 g/mL and above. As a result of the increase in the synthesis of -ca-sein, a milk protein after treatment with MAC-T cells at a concentration of the bee venom without cyto-toxicity, the -casein content in the cell culture was increased when treated at a concentration of 1 ng/mL or more. In addition, it was confirmed that purified bee venom significantly increased the ex-pression of bovine -casein (bCSNB) mRNA, a -casein synthesis gene, at a concentration of 1 ng/mL or more. These results suggest that purified bee venom can be used to increase the production of live-stock by ultimately increasing the expression of milk protein
Abstract
Purified bee venom was collected from colonies of honeybees (Apis mellifera L.) using a bee venom collector under sterile conditions and then purified under strict laboratory conditions. Purified bee venom contained 63.9±5.4% melittin, 10.9±1.6% phospholipase A2, and 2.3±0.3% apamin. Purified bee venom has various anti-bacterial, anti-inflammatory and immunostimulating effects. In this study, we evaluated purified bee venom which are mammary gland cells, MAC-T cells are used to increase the synthesis of milk protein. Purified bee venom promoted the proliferation of MAC-T cells at concentrations below 1 g/mL, but cytotoxicity at 10 g/mL and above. As a result of the increase in the synthesis of -ca-sein, a milk protein after treatment with MAC-T cells at a concentration of the bee venom without cyto-toxicity, the -casein content in the cell culture was increased when treated at a concentration of 1 ng/mL or more. In addition, it was confirmed that purified bee venom significantly increased the ex-pression of bovine -casein (bCSNB) mRNA, a -casein synthesis gene, at a concentration of 1 ng/mL or more. These results suggest that purified bee venom can be used to increase the production of live-stock by ultimately increasing the expression of milk protein
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