도축장 출하차량에서 Lawsonia intracellularis 분포율 조사
Distribution of Lawsonia intracellularis in livestock transport car of slaughterhouse, Korea
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Lawsonia intracellularis is the pathogenic agent of porcine proliferative enteritis (PPE). The bacterial pathogen infects the intestinal crypt cells which causes hyperplasia of the infected cells and leads to the process of intestinal pathogenesis. PPE includes some clinical maninfestations, including acute hem-orrhagic diarrhea with sudden death in growing pigs and porcine intestinal adenomatosis, to a chronic diarrhea with reduced productivity of the infected pigs. The purpose of the present studies were carried out to determine L. intracellularis in livestock transport car of slaughterhouse. Distribution of L. intra-cellularis in livestock transport car were conducted using real-time polymerase chain reaction (real-time PCR) testing method, total 300 samples. Of 300 samples, 119 (39.7%) were detected as positive to L. intracellularis in livestock transport car. In seasonal analysis, 42 (28.0%) out of 150 samples in spring and summer season. 77 (51.3%) out of 150 sample in autumn and winter season. In regional analysis, 53 (88.3%) out of 60 cars and the detection ratio showed that regional variation in livestock transport car.
Abstract
Lawsonia intracellularis is the pathogenic agent of porcine proliferative enteritis (PPE). The bacterial pathogen infects the intestinal crypt cells which causes hyperplasia of the infected cells and leads to the process of intestinal pathogenesis. PPE includes some clinical maninfestations, including acute hem-orrhagic diarrhea with sudden death in growing pigs and porcine intestinal adenomatosis, to a chronic diarrhea with reduced productivity of the infected pigs. The purpose of the present studies were carried out to determine L. intracellularis in livestock transport car of slaughterhouse. Distribution of L. intra-cellularis in livestock transport car were conducted using real-time polymerase chain reaction (real-time PCR) testing method, total 300 samples. Of 300 samples, 119 (39.7%) were detected as positive to L. intracellularis in livestock transport car. In seasonal analysis, 42 (28.0%) out of 150 samples in spring and summer season. 77 (51.3%) out of 150 sample in autumn and winter season. In regional analysis, 53 (88.3%) out of 60 cars and the detection ratio showed that regional variation in livestock transport car.
참고문헌 (25)
- 2016, "2017년 고병원성 조류인플루엔자역학조사 분석 보고서", 농림축산식품부, 2017, pp.55~126
- 2017 구제역 역학조사 분석보고서, "농림축산식품부", 61~67 (2017)
- Cooper DM., "Diagnosis of proliferative enteritis in frozen and formalin-fixed, paraffin embedded tissue from a hamster, horse, deer and ostrich using a Lawsonia intracellularis specific multiplex PCR assay", Vet Microbiol, 54, pp.47~62 (1997)
- Gebhart CJ., "Ileal symbiont intracellularis, an obligate intracellular bacterium of porcine intestines showing a relationship to Desulfovibrio species", Int J Syst Bacteriol, 43, pp.553~538 (1993)
- Gebhart CJ., "Species-specific cloned DNA probe for identification of Campylobacter hyointestinalis", J Clin Microbilol, 27, pp.2717~2723 (1989)
- Guedes RM., "A comparative study of an indirect fluorescent antibody test and an immunoperoxidase monolayer assay for the diagnosis of porcine proliferative enteropathy", J Vet Diagn Invest, 14, pp.420~423 (2002)
- Jones GF., "Transmission of proliferative enteritis to swine by use of embryonating chicken eggs", Am J Vet Res, 54, pp.1256~1261 (1993)
- Jones GF., "Enhanced detection of intracellular organism of swine proliferative enteritis, ileal symbiont intracellularis, in feces by polymerase chain reaction", J Clin Microbiol, 31, pp.2611~2615 (1993)
- 정윤수, "국내 양돈장의 사육구간별 주요 소화기질병 원인체 유병율 조사", 한국동물위생학회지, 39(4), pp.211~219 (2016), 한국동물위생학회
- Lawson GH., "Intracellular bacteria of porcine proliferative enteropathy; cultivation and maintenance in vitro", J Clin Microbiol, 31, pp.1136~1142 (1993)
- Lawson GH., "The enigma of the proliferative enteropathies : a review", J Comp Pathol, 108, pp.41~46 (1993)
- Lee SW., "Prevalence of porcine proliferative enteropathy and its control with tylosin in Korea", J Vet Sci, 2, pp.209~212 (2001)
- Lym SK., "Establishment of diagnostic method for porcine proliferative enteropathy using polymerase chain reaction", Korean J Vet Res, 39, pp.118~125 (1999)
- McCormick BM., "Detection of ileal symbiont intracellularis in porcine faecal sample by polymerase chain reaction", Vet Microbiol, 47, pp.387~393 (1995)
- McOrist S., "Monoclonal antibodies to intracellular Campylobacter-like organisms of the porcine proliferative enteropathies", Vet Rec, 121, pp.421~422 (1987)
- McOrist S., "Polymerase chain reaction for diagnosis of porcine proliferative enteropathy", Vet Microbiol, 41, pp.205~212 (1994)
- McOrist S., "Disease of Swine", pp.727~737 (1999), Wiley-Blackwell
- McOrist S., "Relationship between ileal symbiont intracellularis and porcine proliferative enteritis", Infect Immun, 60, pp.4184~4191 (1993)
- McOrist S., "Synergism of ileal symbiont intracellularis and gut bacteria in the reproduction of porcine proliferative enteropathy", Vet Rec, 134, pp.331~332 (1994)
- 나덕환, "국내 노계 운반 전용 차량에서의 살모넬라 오염율 및 차량소독에 따른 오염도 감소 효과", 한국가금학회지, 40(1), pp.11~16 (2013), 한국가금학회
- 박동엽, "경남지역 내 돼지증식성회장염 감염률 조사 연구", 한국동물위생학회지, 32(4), pp.361~368 (2009), 한국동물위생학회
- Roberts L., "Experimental reproduction of porcine intestinal adenomatosis and necrotic enteritis", Vet Rec, 100, pp.12~13 (1977)
- Roerink F., "A novel inactivated vaccine against Lawsonia intracellularis induces rapid induction of humoral immunity, reduction of bacterial shedding and provides robust gut barrier function", Vaccine, 36, pp.1500~1508 (2018)
- Smith SH., "Development of persistent intestinal infection and excretion of Lawsonia intracellularis by piglets", Res Vet Sci, 60, pp.6~10 (1997)
- Ward GE., "Use of embryonating eggs for isolation of Campylobacter species from intestine of swine with proliferative enteritis", Am J Vet Res, 52, pp.810~812 (1991)