Comparison of Immuno-stimulatory Activities by Purification Process of Schizandra chinensis Baillon Fruits
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Two compounds from Gomisin N and Gomisin A were isolated from the fruits of Schizandra chinensis Bailon. The highest extraction yield as 21.36% was observed in the ethanol extract, compared to the yield obtained from the water extract. The extraction yields of the single compounds were measured to be 0.13 and 0.014 Gomisin A and Gomisin N, respectively. Approximately, 90% of the growth of human stomach adenocarcinoma cancer cells was inhibited after adding 1.0 g/ℓ of the ethanol extract. The growth of the human normal lung cell was limited to 24% after adding the ethanol extract. the water extract lowered the specific secretion of TNF-a' and IL-6 from T cells, 10.3×10-⁴pg/cell and 12.1×10-⁴pg/cell, respectively, compared to the ethanol extracts. On the other hand, a treatment with the ethanol extract increased the specific secretion of TNF-a' and IL-6 from human T cells, to 11×10-⁴pg/cell and 14.3/10-⁴pg/cell, respectively. The crude ethanol extract had the highest effect on the differentiation of human promyelocytic leukemia cells compared to the other extracts and Gomisin A and N. In general, the biological activities of the extracts gradually decreased as the purification process proceeded, which suggests that higher immuno-stimulatory activities can be maintained by adding the crude extracts of the fruits rather than by adding a single compound.
Abstract
Two compounds from Gomisin N and Gomisin A were isolated from the fruits of Schizandra chinensis Bailon. The highest extraction yield as 21.36% was observed in the ethanol extract, compared to the yield obtained from the water extract. The extraction yields of the single compounds were measured to be 0.13 and 0.014 Gomisin A and Gomisin N, respectively. Approximately, 90% of the growth of human stomach adenocarcinoma cancer cells was inhibited after adding 1.0 g/ℓ of the ethanol extract. The growth of the human normal lung cell was limited to 24% after adding the ethanol extract. the water extract lowered the specific secretion of TNF-a' and IL-6 from T cells, 10.3×10-⁴pg/cell and 12.1×10-⁴pg/cell, respectively, compared to the ethanol extracts. On the other hand, a treatment with the ethanol extract increased the specific secretion of TNF-a' and IL-6 from human T cells, to 11×10-⁴pg/cell and 14.3/10-⁴pg/cell, respectively. The crude ethanol extract had the highest effect on the differentiation of human promyelocytic leukemia cells compared to the other extracts and Gomisin A and N. In general, the biological activities of the extracts gradually decreased as the purification process proceeded, which suggests that higher immuno-stimulatory activities can be maintained by adding the crude extracts of the fruits rather than by adding a single compound.