Rapid Propagation of Pelagonium Inquinans Rapid Propagation of Pelagonium Inquinans Rapid Propagation of Pelagonium Inquinans Via Organogenesis fromMature Leaf Explants

Abstract

A method for plant regeneration via organogenesis from Pelagonium inquinans leaf disc has been developed. Mature leaf explants were collected from field-grown plants and used for the induction of adventitious shoot regeneration on Murashige and Skoog (MS) medium supplemented with 3% (w/v) sucrose plus plant growth regulators. Maximum shoot organogenesis, with 11.8 ± 1.5 shoots (98.6%) per leaf disc, was obtained with 2㎎/ℓ N6 -benzyladenine (BA) and 0.5㎎/ℓ α-naphthyleneacetic acid (NAA) in 30 days. For rooting, the in vitro proliferated and elongated shoots were excised into 1.5-2 ㎝ in length microcutting, which were plated individually on an half-strength MS (1/2MS) medium supplemented with 2% (w/v) sucrose plus various concentrations of indole-3-butyric acid (IBA). Shoots rooted with a frequency of 100% following culture on 1/2MS medium containing 0.5㎎/ℓ IBA.