Plant Regeneration through Leaf Explant Culture of Boxthorn (Lycium chinense Mill.)
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Abstract
- This study was conducted to investigate the optimal plant growth regulator level for the shoot formation ofLycium chinenseMill. In vitro plant propagation was developed for leaf explants of boxthorn. Leaf explants werecultured on MS medium supplemented with cytokinins (BA and 2-iP) alone. Plants were successfully regeneratedthrough in vitro culture by using leaf explants of boxthorn grown in the field. After 4 weeks of culture, 58% of shootformation had developed from the leaf explants. The shoot formation rate of ‘Jangmyeong’was highest followed by‘Myeongan’, ‘Cheongdae’, and ‘Bullo’. The use of 0.2mg/L BA was critical for enhanced production of shootformation and resulted in 58% of the culture producing shoot formations. Regenerated plantlets transplanted to pots weredeveloped and successfully acclimatized to greenhouse.Key words - Leaf, shoot formation, Lycium chinenseMill.Korean J. Plant Res. 20(3)：251∼254 (2007)
참고문헌 (14)
- Chungcheongnam-do Agricultural Research & Extension Services, "Research report", pp.773~776 (1997), Chungcheongnam-do Agricultural Research & Extension Services
- Chungcheongnam-do Agricultural Research & Extension Services, "Research report", pp.417~424 (2000), Chungcheongnam-do Agricultural Research & Extension Services
- Chungcheongnam-do Agricultural Research & Extension Services, "Research report", pp.425~432 (2000), Chungcheongnam-do Agricultural Research & Extension Services
- Chungcheongnam-do Agricultural Research & Extension Services, "Research report", pp.373~380 (2004), Chungcheongnam-do Agricultural Research & Extension Services
- Jo, M.H, "High frequency shoot formation and plant regeneration from cotyledonary hypocotyl explants of boxthorn (Lycium chinense Mill.) seedlings", 31, pp.203~207 (2004)
- Kim, D.C, "Plant regeneration from leaf and internode segment cultures of boxthorn (Lycium chinense Mill.)", 28, pp.329~333 (2001)
- Kim S.M, "Agronomic characteristics and changes in chemical components as affected by harvest time in Lycium chinense Mill" (1997)
- Kurokawa S, "General pharmacological studies of the waterand various organic solvent soluble components derived fromLycium chinense", pp.127~136 (1962)
- Lee, B.C, "Production of new cultivar in boxthorn (Lycium chinense Mill.)", pp.417~424 (2000)
- Lee, B.C, "Variation of chemical properties in collected boxthorn varieties", 30, pp.267~272 (1998)
- Lee, "Studies on thetissue culture of Lycium chinense Mill", pp.261~275 (1984), WonkwangUniv
- Skoog, "A revised medium for rapidgrowth and bioassays with tobacco tissue cultures", pp.473~497 (1962)
- Park, "In vitroorganogenesis from leaf callus of Lycium chinense Mill", pp.85~89 (1993)
- Tang, "Chinense drugs of plant origin", pp.633~638 (1986), Springer-Verlag