Evaluation of Sesquiterpenoids Content and Growth Characters inClonal Lines from a Cross between Atractylodes japonicaKoidz. ex Kitam. and A. macrocephala Koidz.
Evaluation of Sesquiterpenoids Content and Growth Characters in Clonal Lines from a Cross between Atractylodes japonica Koidz. ex Kitam. and A. macrocephala Koidz.
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Abstract
Two Atractylodes species, A. japonica Koidz. ex Kitam. (AJ) and A. macrocephala Koidz. (AM) were used in this study. AJ population had higher amounts of sesquiterpenoids and stronger tolerance to root rot but less vigor of root growth than AM population. Two populations (AJ and AM) were crossed to make interspecific hybrid population. A total of 98 lines propagated clonally were selected from a cross of AJ and AM, and evaluated for contents of sesquiterpenoids, atractylon (ATLN) and atractylenolide III (AT3) using high performance liquid chromatography (HPLC), and growth characters such as plant height, stem number and root weight. HPLC profiles of the hybrids were compared with those of parent plants, and it demonstrated the production of introgression hybrid by crossing between AJ and AM. Of 98 clonal lines, 10 lines were selected by 10% level based on the growth vigor and tolerance to root rot, and AJM2102-51 line showed the heaviest root weight (117.1 g/plant) among them. A total of 98 hybrid lines contained on average 0.16 ± 0.10㎎/g of AT3, 2.00 ± 1.37㎎/g of ATLN, and 2.16 ± 1.40㎎/g of total sesquiterpenoids, showing high coefficients of variation (above 65 %). Ten lines having high contents of sesquiterpenoids were selected, and AJM2101-15 had the highest amount (9.83㎎/g) of ATLN, and showed 40.8 g/ plant of root weight similar to mean value (39.9 g/plant) of hybrid lines. The result showed that the introgression of both characters of vigorous growth from AM and high sesquiterpenoids content from AJ could be possible to make new hybrid lines by crossing between AJ and AM.
참고문헌 (13)
- kyonghwanbang, "Discrimination of species specific DNA Markers using RAPD and SFLP analysis between Atractylodes japonica Koidz.", 한국약용작물학회지, 11(4), pp.268~273 (2003), 한국약용작물학회
- Cho JH, "Genetic variation of Atractylodes macrocephala in morphological traits and Phytophthora root rot resistance", Kor. J. Breed, 33, pp.191~198 (2001)
- Jin Hong Park, "Comparison of Immuno-stimulatory Activities by Purification Process of Schizandra chinensis Baillon Fruits", 한국약용작물학회지, 12(2), pp.141~148 (2004), 한국약용작물학회
- Fukuda T, "Studies of cultivation of Atractylodes ovata. III. Hybridization and the change of morphological characteristics caused by the hybridization", Natural Medicines, 49(4), pp.431~437 (1995)
- Fukuda T, "Studies of cultivation of Atractylodes ovata. VI. Influence of cultivation method on morphology of crude drug and contents of chemical substances of each part of crude drug", Natural Medicines, 52(5), pp.390~395 (1998)
- Fukuda T, "Studies of cultivation of Atractylodes ovata. IV. Individual variation of external morphology and individual difference of amount of growth and contents of chemical substances", Natural Medicines, 51(5), pp.420~426 (1997)
- Huh HW, "Genetic diversity and population structure of Atractylodes japonica Koidz. in Korea", Kor. J. Medicinal Crop Sci, 10(1), pp.5~11 (2002)
- Kim JK, "Variation analysis of Atractylodes japonica Koidzumi ex Kitamura based on quantitative characters", Kor. J. Plant Res, 15(1), pp.36~42 (2002)
- Shiba M, "Identification of medicinal Atractylodes based on ITS sequences of nrDNA", Biol. Pharm. Bull, 29(2), pp.315~320 (2006)
- Suto K, "Determination of atractylon in Atractylodes rhizome using supercritical fluid chromatography on-line coupled with supercritical fluid extraction by the direct induction method", J. Chromatography A, 810, pp.252~255 (1998)
- Tang, W, "Atractylodes macrocephala Koidz. In: Chinese drugs of plant origin", pp.199~201 (1992), Springer-Verlag, Berlin
- Wang CC, "Cytotoxic activity of sesquiterpenoids from Atractylodes ovata on leukemia cell lines", Planta Med, 68, pp.204~208 (2002)
- Wang R, "Quantitative determination of atractylenolide III in rat plasma by liquid chromatography electrospray ionization mass spectrometry", J. Chromatography B, 831, pp.36~41 (2006)