Discrimination of species specific DNA Markers using RAPD and SFLP analysis between Atractylodes japonica Koidz.

Abstract

To identify the variation of the RAPD patterns between two Atracylodes species, 52 kinds of random primers were applied to each eight of A. japonica and A. macrocephala genomic DNA. Ten primers of 52 primers could be used to dixcrminate between the species and 18 polymorphi는 among 67 scored DNA fragments (18 fragments are specific for A. japonica and A. macrocephala) were generated using these primers, 26.9% of which were polymorphic. RAPD data from the 10 primers was used for cluster analysis. The cluster analysis of FAPD markers showed that the two groups are genetically distinct. On the other hand, to identify the variation of the AFLP patterns and select the species specific AFLP markers, eight combinations of EcoRI/Mse I primers (EcoR I/Mse I ; AAC/CTA, AAC/CAA, AAG/CTA)used in this study revealed 176 reliable AFLP markers, 42.0% of which were polymorphic. 74 polmorphisms out of 176 scored 움 fragments were enough to clearly discriminate between two Atractylodes species.