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Suppression Effect of the Inflammatory Response in Macrophages by Paeoniae Radix Rubra Extracts

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Paeoniae Radix Rubra is a preparation consisting of desiccated roots of Paeonia lactiflora PALL (belonging to Ranunculaceae). Paeoniae Radix Rubra is used as a medicinal herb in Asian countries to treat many diseases. Ethanol- or water-based extracts of Paeoniae Radix Rubra were prepared and tested on RAW 264.7 cells, a murine macrophage cell line. The expression of some pro-inflammatory proteins, including inducible nitric oxide synthase (iNOS), cyclooxygenase-2(COX-2), extracellular signal-regulated kinase 1/2 (ERK1/2) and phosphorylated ERK1/2 was detected by Western blot analyses, while PGE2 expression was quantified by ELISA. Both the water and ethanol extracts of Paeoniae Radix Rubra suppressed LPS-induced nitric oxide (NO) production and exhibited cell toxicity in accordance with increased NO production. Also, both extracts reduced the expression of COX-2 and iNOS, and inhibited phosphorylation of ERK1/2 in LPS-stimulated RAW 264.7 cells. Extracts prepared from Paeoniae Radix Rubra contain anti-inflammatory agents that inhibit the iNOS and MAPK pathways.

Abstract

Paeoniae Radix Rubra is a preparation consisting of desiccated roots of Paeonia lactiflora PALL (belonging to Ranunculaceae). Paeoniae Radix Rubra is used as a medicinal herb in Asian countries to treat many diseases. Ethanol- or water-based extracts of Paeoniae Radix Rubra were prepared and tested on RAW 264.7 cells, a murine macrophage cell line. The expression of some pro-inflammatory proteins, including inducible nitric oxide synthase (iNOS), cyclooxygenase-2(COX-2), extracellular signal-regulated kinase 1/2 (ERK1/2) and phosphorylated ERK1/2 was detected by Western blot analyses, while PGE2 expression was quantified by ELISA. Both the water and ethanol extracts of Paeoniae Radix Rubra suppressed LPS-induced nitric oxide (NO) production and exhibited cell toxicity in accordance with increased NO production. Also, both extracts reduced the expression of COX-2 and iNOS, and inhibited phosphorylation of ERK1/2 in LPS-stimulated RAW 264.7 cells. Extracts prepared from Paeoniae Radix Rubra contain anti-inflammatory agents that inhibit the iNOS and MAPK pathways.

참고문헌 (43)

  1. Adams DO, "The cell biology of macrophage activation", Annual Review of Immunology, 2, pp.283~318 (1984)
  2. Adams V, "Increased inducible nitric oxide synthase in skeletal muscle biopsies from patients with chronic heart failure", Biochemical and Molecular Medicine, 61, pp.152~160 (1997)
  3. Aderem A, "Phagocytosis and the inflammatory response", The Journal of Infectious Diseases, 187, pp.S340~S345 (2003)
  4. Bredt DS, "Nitric oxide mediates glutamate-linked enhancement of cGMP levels in the cerebellum", Proceedings of the National Academy of Sciences of the United States of America, 86, pp.9030~9033 (1989)
  5. Bredt DS, "Transient nitric oxide synthase neurons in embryonic cerebral cortical plate, sensory ganglia, and olfactory epithelium", Neuron, 13, pp.301~313 (1994)
  6. Calderón-Garcidueñas L, "Brain inflammation and Alzheimer`s-like pathology in individuals exposed to severe air pollution", Toxicologic Pathology, 32, pp.650~658 (2004)
  7. Chen C, "Protective effect of pretreatment with Radix Paeoniae Rubra on acute lung injury induced by intestinal ischemia", reperfusion in rats, 2008, pp.11, 37~41
  8. Chan SC, "Altered prostaglandin E2 regulation of cytokine production in atopic dermatitis", Journal of Immunology, 151, pp.3345~3352 (1993)
  9. Stacia Clement-Kruzel, "Immune Modulation of Macrophage Pro-Inflammatory Response by Goldenseal and Astragalus Extracts", Journal of Medicinal Food, 11(3), pp.493~498 (2008), 한국식품영양과학회
  10. Culbert AA, "MAPK-activated protein kinase 2 deficiency in microglia inhibits pro-inflammatory mediator release and resultant neurotoxicity. Relevance to neuroinflammati on in a transgenic mouse model of Alzheimer disease", The Journal of Biological Chemistry, 281, pp.23658~23667 (2006)
  11. Guan Z, "Interleukin-1beta-induced cyclooxygenase-2 expression requires activation of both c-Jun NH2-terminal kinase and p38 MAPK signal pathways in rat renal mesangial cells", The Journal of Biological Chemistry, 273, pp.28670~28676 (1998)
  12. Grnblatt E, "Neuroprotective strategies in Parkinson`s disease using the models of 6- hydroxydopamine and MPTP", Annals of the New York Academy of Sciences, 889, pp.262~273 (2000)
  13. Hibbs JB, "Macrophage cytotoxicity: role of L-arginine deiminase and imino nitrogen oxidation to nitrite", Science, 235, pp.473~476 (1987)
  14. Jeremy JY, "Nitric oxide and the proliferation of vascular smooth muscle cells", Cardiovascular Research, 43, pp.580~594 (1999)
  15. Jung HW, "Bisabolangelone isolated from Ostericum koreanum inhibits the production of inflammatory mediators by down-regulation of NF-kappa B and ERK MAP kinase activity in LPS-stimulated RAW264.7 cells", INTERNATIONAL IMMUNOPHARMACOLOGY, 10, pp.155~162 (2010), ELSEVIER SCIENCE BV
  16. 김세종, "작약 잎과 줄기의 생리활성 물질 분리 및 동정", 한국약용작물학회지, 15(1), pp.6~11 (2007), 한국약용작물학회
  17. Kock A, "Human keratinocytes are a source for tumor necrosis factor-α: evidence for synthesis and release upon stimulation with endotoxin or ultraviolet light", Journal of Experimental Medicine, 172, pp.1609~1614 (1990), .
  18. Lawrence T, "Antiinflammatory lipid mediators and insights into the resolution of inflammation", Nature Reviews Immunology, 2, pp.787~795 (2002)
  19. 이승은, "약용식물자원 추출물의 항염증활성", 한국약용작물학회지, 19(4), pp.217~226 (2011), 한국약용작물학회
  20. Lipton SA, "A redox-based mechanism for the neuroprotective and neurodestructive effects of nitric oxide and related nitroso-compouds", Nature, 364, pp.626~632 (1993)
  21. Lowenstein CJ, "Nitric oxide inhibits viral replication in murine myocarditis", The Journal of Clinical Investigation, 97, pp.1837~1843 (1996)
  22. Mahut B, "Both inflammation and remodeling influence nitric oxide output in children with refractory asthma", The Journal of Allergy and Clinical Immunology, 113, pp.252~256 (2004)
  23. McGinty A, "Induction of prostaglandin endoperoxide synthase 2 by mitogen-activated protein kinase cascades", The Biochemical Journal, 352, pp.419~424 (2000)
  24. Nathan CF, "Massive secretion of hydrogen peroxide in response to products of macrophages and lymphocytes", The Journal of Clinical Investigation, 80, pp.1550~1560 (1987)
  25. Puckett JL, "An elevated bronchodilator response predicts large airway inflammation in mild asthma", Pediatric Pulmonology, 45, pp.174~181 (2010)
  26. Palmer RM, "Vascular endothelial cells synthesize nitric oxide from L-arginine", Nature, 333, pp.664~666 (1988)
  27. Palmer RM, "A novel citrulline-forming enzyme implicated in the formation of nitric oxide by vascular endothelial cells", Biochemical and Biophysical Research Communications, 158, pp.348~352 (1989)
  28. Ridnouur LA, "The biphasic nature of nitric oxide responses in tumor biology", Antioxidants & Redox Signaling, 8, pp.1329~1337 (2006)
  29. Rodriguez-Barbero A, "TGF-β1 induces COX-2 expression and PGE2 synthesis through MAPK and PI3K pathways in human mesangial cells", Kidney International, 70, pp.901~909 (2006)
  30. Schmidt H, "NO at work", Cell, 78, pp.919~925 (1994)
  31. Schneider A, "Nephrology, dialysis, transplantation:official publication of the European Dialysis and Transplant Association-European Renal Association", Nephrology, dialysis, transplantation:official publication of the European Dialysis and Transplant Association-European Renal Association, 13, pp.10~12 (1998)
  32. Selkoe DJ, "Alzheimer`s disease: genes, protein, and therapy", Physiological Reviews, 81, pp.741~766 (2001)
  33. Sessa WC, "The nitric oxide synthase family of proteins", aJournal of Vascular Research, 31, pp.131~143 (1994)
  34. Skidgel RA, "Nitric oxide stimulates macrophage inflammatory protein2 expression in sepsis", Journal of Immunology, 169, pp.2093~2101 (2002)
  35. Sun WY, "Protective effect of extract from Paeonia lactiflora and Astragalus membranaceus against liver injury induced by Bacillus Calmette-Guerin and lipopolysaccharide in mice", Basic & Clinical Pharmacology & Toxicology, 103, pp.143~149 (2008)
  36. Tetsuka T, "Nitric oxide amplifies interleukin 1- induced cyclooxygenase-1 expression in rat mesangial cells", The Journal of Clinical Investigation, 97, pp.2051~2056 (1996)
  37. Torres SH, "Inflammation and nitric oxide production in skeletal muscle of type 2 diabetic patient", The Journal of Endocrinology, 181, pp.419~427 (2004)
  38. Williams CS, "Prostaglandin endoperoxide synthase: why two isoforms", The American Journal of Physiology, 270, pp.G393~G400 (1996)
  39. Wu GJ, "Ketamine inhibits tumor necrosis factor-α and interleukin-6 gene expressions in lipopolysaccharide-stimulated macrophages through suppression of toil-like receptor 4-mediated c-Jin N terminal kinase phosphorylation and activator protein-1 activation", Toxicology and Applied Pharmacology, 228, pp.105~113 (2008)
  40. Wu KK, "Cyclooxygenase 2 induction: molecular mechanism and pathophysiologic roles", The Journal of Laboratory and Clinical Medicine, 128, pp.242~245 (1996)
  41. Wu SH, "Chemical constituents and bioactivities of plants from the genus Paeonia", Chemistry & Biodiversity, 7, pp.90~104 (2010)
  42. 윤태숙, "Evaluation of Solvent Extraction on the Anti-Inflammatory Efficacy of Glycyrrhiza uralensis", 한국약용작물학회지, 18(1), pp.28~33 (2010), 한국약용작물학회
  43. Zhan LY, "Effect of Radix Paeoniae Rubra on the expression of HO-1 and iNOS in rats with endotoxin-induced acute lung injury", Chinese Journal of Traumatology, 9, pp.181~186 (2006)

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