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Anti-inflammatory Effects of Abeliophyllum distichum Flower Extract and Associated MAPKs and NF-κB Pathway in Raw264.7 Cells

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Abeliophyllum distichum is a medicinal plant used in regional traditional medicine to relieve pain in inflammatory processes. In this study, anti-inflammatory effects of Abeliophyllum distichum flower (ADF) extract were examined. Furthermore, possible molecular mechanisms of the anti-inflammatory effects were dissected. The anti-inflammatory activity was investigated by inhibition of lipopolysaccharide (LPS) induced pro-inflammatory cytokine production in murine macrophage-like cell line Raw264.7 cells. The measurement of the induced pro-inflammatory cytokine levels were carried out by ELISA. The phosphorylation of ERK1/2, JNK, and MAPK, and the nuclear expression of nuclear factor NF-κ B p65 were investigated by Western blot analysis. The extract of ADF significantly decreased the production of pro-inflammatory cytokines. In addition, the extract suppressed the phosphorylation of ERK1/2, JNK, and p38 MAPK, and the nuclear translocation of NF-κB p65 in activated cells. Our findings provide evidence for the popular use of Abeliophylli distichum in inflammation around Goesan region and also suggest that the flower extract has potential therapeutic benefits against various inflammatory diseases.

Abstract

Abeliophyllum distichum is a medicinal plant used in regional traditional medicine to relieve pain in inflammatory processes. In this study, anti-inflammatory effects of Abeliophyllum distichum flower (ADF) extract were examined. Furthermore, possible molecular mechanisms of the anti-inflammatory effects were dissected. The anti-inflammatory activity was investigated by inhibition of lipopolysaccharide (LPS) induced pro-inflammatory cytokine production in murine macrophage-like cell line Raw264.7 cells. The measurement of the induced pro-inflammatory cytokine levels were carried out by ELISA. The phosphorylation of ERK1/2, JNK, and MAPK, and the nuclear expression of nuclear factor NF-κ B p65 were investigated by Western blot analysis. The extract of ADF significantly decreased the production of pro-inflammatory cytokines. In addition, the extract suppressed the phosphorylation of ERK1/2, JNK, and p38 MAPK, and the nuclear translocation of NF-κB p65 in activated cells. Our findings provide evidence for the popular use of Abeliophylli distichum in inflammation around Goesan region and also suggest that the flower extract has potential therapeutic benefits against various inflammatory diseases.

참고문헌 (41)

  1. Aderem, A., "Toll-like receptors in the induction of the innate immune response", Nature, 406, pp.782~787 (2000)[DOI]
  2. Amalinei, C., "Biology of metalloproteinases", Rom J Morphol Embryol, 48, pp.323~334 (2007)
  3. Axtelle, T., "IC14, a CD14 specific monoclonal antibody, is a potential treatment for patients with severe sepsis", J Endotoxin Res, 7, pp.310~314 (2001)[DOI]
  4. Bendtzen, K, "Special Issue: Second Meeting on Infection Immunity Elsinore, Denmark, 17-21 June 1988Interleukin 1, interleukin 6 and tumor necrosis factor in infection, inflammation and immunity", Immunol Lett, 19, pp.183~192 (1988)[DOI]
  5. Bhattacharyya, A., "Mitogen-activated protein kinases and NF-kappaB regulate H. pylori-mediated IL-8 release from macrophages", Biochem J, 366, pp.376~382 (2002)
  6. Binetruy, B., "Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain", Nature, 351, pp.122~127 (1991)[DOI]
  7. Bosca, L., "Nitric oxide and cell viability in inflammatory cells: a role for NO in macrophage function and fate", Toxicology, 208, pp.249~258 (2005)[DOI]
  8. Coussens, L.M., "Matrix metalloproteinase inhibitors and cancer-trials and tribulations", Science, 295, pp.2387~2392 (2002)[DOI]
  9. Farabegoli, F., "(-)-Epigallocatechin-3-gallate down-regulates EGFR, MMP-2, MMP-9 and EMMPRIN and inhibits the invasion of MCF-7 tamoxifen-resistant cells", Biosci Rep, 31, pp.99~108 (2011)[DOI]
  10. Festuccia, C, "Osteoblasts modulate secretion of urokinase-type plasminogen activator (uPA) and matrix metalloproteinase-9 (MMP-9) in human prostate cancer cells promoting migration and matrigel invasion", Oncol Res, 11, pp.17~31 (1998)
  11. Fujiwara, N., "Macrophages in inflammation", Curr Drug Targets Inflamm Allergy, 4, pp.281~286 (2005)[DOI]
  12. Ghosh, S., "New regulators of <TEX>$NF-{\kappa}B$<", TEX> in inflammation, 2008, pp.8, 837~848
  13. Grivennikov, S.I., "Immunity, inflammation, and cancer", Cell, 140, pp.883~899 (2010)[DOI]
  14. Guo Huifang, "Anti-inflammatory and Anti-cancer Effect of Stachys affinis Tubers", 한국자원식물학회지, 30(6), pp.679~685 (2017), 한국자원식물학회[DOI]
  15. Herlaar, E., "p38 MAPK signalling cascades in inflammatory disease", Mol Med Today, 5, pp.439~447 (1999)[DOI]
  16. Hibi, M., "IL-6 cytokine family and signal transduction: a model of the cytokine system", J Mol Med, 74, pp.1~12 (1996)[DOI]
  17. Hoshino, K, "Cutting edge: Toll-like receptor 4 (TLR4)-deficient mice are hyporesponsive to lipopolysaccharide: evidence for TLR4 as the Lps gene product", J Immunol, 162, pp.3749~3752 (1999)
  18. Hung, W.-C., "Skp2 overexpression increases the expression of MMP-2 and MMP-9 and invasion of lung cancer cells", Cancer Lett, 288, pp.156~161 (2010)[DOI]
  19. Ide, N., "Garlic compounds minimize intracellular oxidative stress and inhibit nuclear <TEX>$factor-{\kappa}B$<", TEX> activation, 2001, pp.131, 1020S~1026S
  20. Iwalewa, E., "Inflammation: the foundation of diseases and disorders. A review of phytomedicines of South African origin used to treat pain and inflammatory conditions", Afri J Biotech, 6(25), pp.2868~2885 (2007)[DOI]
  21. Johnson, G.L., "Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases", Science, 298, pp.1911~1912 (2002)[DOI]
  22. Lazarov, S., "The role of bacterial endotoxins, receptors and cytokines in the pathogenesis of septic (endotoxin) shock", Vutr Boles, 32, pp.33~40 (1999)
  23. Lee, E.-J., "Glycitein inhibits glioma cell invasion through down-regulation of MMP-3 and MMP-9 gene expression", Chem Biol Interact, 185, pp.18~24 (2010)[DOI]
  24. Tae Hoon Lee, "Methanol Extracts of Stewartia koreana Inhibit Cyclooxygenase-2 (COX-2) and Inducible Nitric Oxide Synthase (iNOS) Gene Expression by Blocking NF-κB Transactivation in LPS-activated RAW 264.7 Cells", Molecules and Cells, 23(3), pp.398~404 (2007), 한국분자세포생물학회
  25. Li, Q., "<TEX>$NF-{\kappa}B$<", TEX> regulation in the immune system, 2002, pp.2, 725~734
  26. Libby, P., "Inflammation and atherosclerosis", Circulation, 105, pp.1135~1143 (2002)[DOI]
  27. Ljung, T., "Rectal nitric oxide as biomarker in the treatment of inflammatory bowel disease: responders versus nonresponders", World J Gastroenterol, 12, pp.3386~ (2006)[DOI]
  28. Majdalawieh, A., "Regulation of IkappaBalpha function and NF-kappaB signaling: AEBP1 is a novel proinflammatory mediator in macrophages", Mediators Inflamm, 2010, pp.823821~ (2010)
  29. Nair, V.D., "Early single cell bifurcation of pro-and antiapoptotic states during oxidative stress", J Biol Chem, 279, pp.27494~27501 (2004)[DOI]
  30. Nalla, A.K., "Targeting MMP-9, uPAR, and cathepsin B inhibits invasion, migration and activates apoptosis in prostate cancer cells", Cancer Gene Ther, 17, pp.599~613 (2010)[DOI]
  31. Natarajan, K., "Protein tyrosine kinase inhibitors block tumor necrosis factor-induced activation of nuclear <TEX>$factor-{\kappa}B$<", TEX>, degradation of <TEX>$I{\kappa}B{\alpha}$<, Arch Biochem Biophys, pp.1998, 352[DOI]
  32. Opal, S.M., "Anti-inflammatory cytokines", Chest, 117, pp.1162~1172 (2000)[DOI]
  33. 박광훈, "Anti-Inflammatory Effect of the Extracts from Abeliophyllum distichum Nakai in LPS-Stimulated RAW264.7 Cells", 한국자원식물학회지, 27(3), pp.209~214 (2014), 한국자원식물학회[DOI]
  34. 박재호, "연자육 추출물의 항산화 및 산화적 DNA 손상억제 활성", 대한본초학회지, 25(4), pp.55~59 (2010), 대한본초학회
  35. Pasparakis, M., "Immune and inflammatory responses in TNF alpha-deficient mice: a critical requirement for TNF alpha in the formation of primary B cell follicles, follicular dendritic cell networks and germinal centers, and in the maturation of the humoral immune response", J Exp Med, 184, pp.1397~1411 (1996)[DOI]
  36. Ramos-DeSimone, N., "Activation of matrix metalloproteinase-9 (MMP-9) via a converging plasmin", stromelysin-1 cascade enhances tumor cell invasion, 1999, pp.274, 13066~13076
  37. Ryan, G.B., "Acute inflammation. A review", Am J Pathol, 86, pp.183~ (1977)
  38. Sil, H., "Fibronectin‐Integrin (<TEX>${\alpha}5{\beta}1$<", TEX>) Modulates migration and invasion of murine melanoma cell line B16F10 by involving MMP-9, 2011, pp.19, 335~348
  39. Vallabhapurapu, S., "Regulation and function of <TEX>$NF-{\kappa}B$<", TEX> transcription factors in the immune system, 2009, pp.27, 693~733
  40. Visse, R., "Matrix metalloproteinases and tissue inhibitors of metalloproteinases structure, function, and biochemistry", Circ Res, 92, pp.827~839 (2003)[DOI]
  41. Xin, W., "Effect of osteoprotegerin in combination with interleukin-6 on inhibition of osteoclast differentiation", Chin J Traumatol, 16, pp.277~280 (2013)

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