더덕 잎, 줄기, 뿌리 부위의 Triterpenoid 사포닌 함량
Triterpenoid Saponin Contents of the Leaf, Stem and Root of Codonopsis lanceolata
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초록
Codonopsis lanceolata (Campanulaceae) has been used in traditional medicines, as its roots contain severalkinds of 3,28-bidesmosidic triterpenoid saponin with high medicinal values. In this study, we induced hairy root-derivedtransgenic plants of C. lanceolata and analyzed triterpenoid saponins from the leaf, stem and root. Transgenic plants wereregenerated from the hairy roots via somatic embryogenesis. The saponins are lancemaside A, B and E, foetidissimoside A,and aster saponin Hb. Transgenic plants contained richer triterpenoids saponin than wild-type plants. Major saponin lancemasideA was the most abundant saponin in the stem from transgenic-plant, 4.76 ㎎·1−1 dry stem. These results suggest thattransgenic plants of C. lanceolata could be used as medicinal materials for the production of triterpene saponins.
Abstract
Codonopsis lanceolata (Campanulaceae) has been used in traditional medicines, as its roots contain severalkinds of 3,28-bidesmosidic triterpenoid saponin with high medicinal values. In this study, we induced hairy root-derivedtransgenic plants of C. lanceolata and analyzed triterpenoid saponins from the leaf, stem and root. Transgenic plants wereregenerated from the hairy roots via somatic embryogenesis. The saponins are lancemaside A, B and E, foetidissimoside A,and aster saponin Hb. Transgenic plants contained richer triterpenoids saponin than wild-type plants. Major saponin lancemasideA was the most abundant saponin in the stem from transgenic-plant, 4.76 ㎎·1−1 dry stem. These results suggest thattransgenic plants of C. lanceolata could be used as medicinal materials for the production of triterpene saponins.
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