Comparative Analysis of Metabolites in Roots of Panax ginseng Obtained from Different Sowing Methods
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초록
Ginsenosides of roots in Panax ginseng were analyzed by metabolic-targeting HPLC using the partial leastsquares discriminant analysis (PLS-DA) and compared depending on sowing methods between direct seeding andtransplanting method. Score plots derived from PLS-DA could identify the sowing method between the direct seeding andtransplanting method in P. ginseng roots. The ginsenoside compounds were assigned as Rg1, Re, Rf, Rg2, Rb1, Rc,Rb2, Rb3, and Rd. Contents of Re, Rf, Rg2, Rb1, Rc, Rb3, and Rd of main roots produced from the transplanting methodwere relatively higher than those of samples produced from direct seeding method. Also, contents of Rg1, Re, Rf, Rg2, Rb1,Rc, Rb2, Rb3, and Rd of lateral roots from the transplanted samples were relatively higher than those of samples producedfrom direct seeding method. Therefore, HPLC with PLS-DA analysis can be a straightforward tool for identificationof ginsenosides in main or lateral roots of P. ginseng obtained from two different seeding methods between directand transplanting methods.
Abstract
Ginsenosides of roots in Panax ginseng were analyzed by metabolic-targeting HPLC using the partial leastsquares discriminant analysis (PLS-DA) and compared depending on sowing methods between direct seeding andtransplanting method. Score plots derived from PLS-DA could identify the sowing method between the direct seeding andtransplanting method in P. ginseng roots. The ginsenoside compounds were assigned as Rg1, Re, Rf, Rg2, Rb1, Rc,Rb2, Rb3, and Rd. Contents of Re, Rf, Rg2, Rb1, Rc, Rb3, and Rd of main roots produced from the transplanting methodwere relatively higher than those of samples produced from direct seeding method. Also, contents of Rg1, Re, Rf, Rg2, Rb1,Rc, Rb2, Rb3, and Rd of lateral roots from the transplanted samples were relatively higher than those of samples producedfrom direct seeding method. Therefore, HPLC with PLS-DA analysis can be a straightforward tool for identificationof ginsenosides in main or lateral roots of P. ginseng obtained from two different seeding methods between directand transplanting methods.
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