섬기린초(Sedum takesimense)에서 분리된 화합물들의 RAW 264.7 Cell에 대한 항염증 효과와 여드름 원인균에 대한 항균 효과
Anti-inflammatory Effect on RAW 264.7 Cells and Antibacterial Effect on Cutibacterium acnes of Compounds Isolated from Sedum takesimense
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This study investigated anti-inflammatory and antibacterial activities of Sedum takesimense ethanolic extract, and3 isolated compounds. To confirm anti-inflammatory and anti-acne activities, a nitric oxide (NO) inhibition assay,pro-inflammatory factor (TNF-α, IL-1β, and IL-8) inhibition assays, and minimum inhibitory concentration (MIC)tests were performed. The 3 isolated compounds were identified as 4,6-di-O-galloylarbutin (OGA), 2,4,6-tri-O-galloyl-glucose (OGG), and 1,2,4,6-tetra-O-galloyl-β-glucose (TOGG). The ethanolic extract and isolated compounds(OGA, OGG, TOGG) effectively inhibited production of NO and pro-inflammatory cytokines (TNF-α, IL-1β, andIL-8). Furthermore, OGG and TOGG exhibited MIC values toward Cutibacterium acnes of 12.5 μg/mL and 3.2 μg/mL, respectively. These results suggest that S. takesimense extract exerts an anti-inflammatory effect on LPS-inducedRAW264.7 cells, and an antibacterial efficacy against C. acnes.
Abstract
This study investigated anti-inflammatory and antibacterial activities of Sedum takesimense ethanolic extract, and3 isolated compounds. To confirm anti-inflammatory and anti-acne activities, a nitric oxide (NO) inhibition assay,pro-inflammatory factor (TNF-α, IL-1β, and IL-8) inhibition assays, and minimum inhibitory concentration (MIC)tests were performed. The 3 isolated compounds were identified as 4,6-di-O-galloylarbutin (OGA), 2,4,6-tri-O-galloyl-glucose (OGG), and 1,2,4,6-tetra-O-galloyl-β-glucose (TOGG). The ethanolic extract and isolated compounds(OGA, OGG, TOGG) effectively inhibited production of NO and pro-inflammatory cytokines (TNF-α, IL-1β, andIL-8). Furthermore, OGG and TOGG exhibited MIC values toward Cutibacterium acnes of 12.5 μg/mL and 3.2 μg/mL, respectively. These results suggest that S. takesimense extract exerts an anti-inflammatory effect on LPS-inducedRAW264.7 cells, and an antibacterial efficacy against C. acnes.
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