심재, 변재, 형성층 및 사부를 포함한 뽕나무 심부 고온추출물의 항염증 활성
Anti-Inflammatory Activity of High Temperature Extract from the Central Part Including Heartwood, Sapwood, Cambiun, and Phloem in Morus alba L.
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Background: Inflammation plays an important role in various diseases, including ulcerative colitis, Behcet's disease, and rheumatoid arthritis. In this study, we investigated the anti-inflammatory effects of Morus alba L. extracts obtained using different extraction methods (water extraction or high temperature extraction) on RAW264.7 cells. Methods and Results: Extracts from the central part (including the heartwood, sapwood, cambiun, and phloem) and bark (including the periderm and cortex) of Morus alba L. were obtained using either water or high temperature extraction. The following extract were obtained: MA1, water extract from the central part of Morus alba L., MA2, high temperature extract from the central part of Morus alba L., MA3, water extract from the bark of Morus alba L., and MA4, high temperature extract from the bark of Morus alba L. None of these extracts was observed to be cytotoxic to RAW264.7 cells. The MA2 extract reduced the production of LPS-induced NO (nitric oxide), PGE2 (prostaglandin E2), TNF-α, IL-6, and IL-1β production in LPS-stimulated RAW264.7 cells. Conclusions: These results indicated that the inflammatory response was moderated by MA2. Treatment with MA2 could be used as a natural medicine for treating diseases involving inflammation. However, further experiments are required to determine how the high temperature extraction method alters the active ingredients in the extract and influences the anti-inflammatory effects of Morus alba L..
Abstract
Background: Inflammation plays an important role in various diseases, including ulcerative colitis, Behcet's disease, and rheumatoid arthritis. In this study, we investigated the anti-inflammatory effects of Morus alba L. extracts obtained using different extraction methods (water extraction or high temperature extraction) on RAW264.7 cells. Methods and Results: Extracts from the central part (including the heartwood, sapwood, cambiun, and phloem) and bark (including the periderm and cortex) of Morus alba L. were obtained using either water or high temperature extraction. The following extract were obtained: MA1, water extract from the central part of Morus alba L., MA2, high temperature extract from the central part of Morus alba L., MA3, water extract from the bark of Morus alba L., and MA4, high temperature extract from the bark of Morus alba L. None of these extracts was observed to be cytotoxic to RAW264.7 cells. The MA2 extract reduced the production of LPS-induced NO (nitric oxide), PGE2 (prostaglandin E2), TNF-α, IL-6, and IL-1β production in LPS-stimulated RAW264.7 cells. Conclusions: These results indicated that the inflammatory response was moderated by MA2. Treatment with MA2 could be used as a natural medicine for treating diseases involving inflammation. However, further experiments are required to determine how the high temperature extraction method alters the active ingredients in the extract and influences the anti-inflammatory effects of Morus alba L..
참고문헌 (31)
- Bergeron C, "Comparison of the chemical composition of extracts from Scutellaria lateriflora using accelerated solvent extraction and supercritical fluid extraction versus standard hot water or 70% ethanol extraction", Journal of Agricultural and Food Chemistry, 53, pp.3076~3080 (2005)[DOI]
- Bradley JR, "TNF-mediated inflammatory disease", Journal of Pathology, 214, pp.149~160 (2008)[DOI]
- Guslandi M, "Nitric oxide and inflammatory bowel disease", European Journal of Clinical Investigation, 28, pp.904~907 (1998)[DOI]
- 임도연, "화피의 휘발성 향기성분 조성과 주요성분인 Benzaldehyde의 생리활성", 아시안뷰티화장품학술지, 12(2), pp.155~162 (2014), 한국피부과학연구원
- 지종대, "Prostaglandin E2: 면역 및 염증반응에서의 역할", 대한류마티스학회지, 11(4), pp.307~316 (2004), 대한류마티스학회
- Katherine LSV, "Extraction conditions affecting supercritical fluid extraction(SFE) of lycopene from watermelon", Bioresource Technology, 99, pp.7835~7841 (2008)[DOI]
- 김미원, "식용대두유에 대한 뽕잎추출물의 항산화 작용", 한국식생활문화학회지, 18(1), pp.1~8 (2003), 한국식생활문화학회
- 김영경, "NITRIC OXIDE와 치수", Restorative Dentistry and Endodontics, 27(5), pp.543~551 (2002), 대한치과보존학회
- 권영주, "마이크로웨이브 추출조건에 따른 곰취 추출물의 총 폴리페놀 함량 및 항산화작용의 변화", Food Science and Preservation, 9(3), pp.332~337 (2002), 한국식품저장유통학회
- Lalonde S, "Transport mechanisms for organic forms of carbon and nitrogen between source and sink", Annual Review of Plant Biology, 55, pp.341~372 (2004)[DOI]
- 이강선, "추출방법에 따른 홍삼추출액의 사포닌 조성과 품질특성", 한국약용작물학회지, 21(4), pp.276~281 (2013), 한국약용작물학회[DOI]
- 이한나, "등골나물 추출물의 항염증 효과", 한국식품과학회지, 43(1), pp.65~71 (2011), 한국식품과학회
- 이숙현, "감귤류 과피 추출물의 항염증 효과", 한국식품위생안전성학회지, 28(4), pp.342~348 (2013), 한국식품위생안전성학회
- Moncade S, "Nitric oxide: Physiology, pathophysiology, and phamacology", Pharmacological Reviews, 43, pp.109~142 (1991)
- Nathan C, "Nitric oxide as a secretory product of mammalian cells", The FASEB Journal, 6, pp.3051~3064 (1992)[DOI]
- Nathan C, "Points of control in inflammation", Nature, 420, pp.846~852 (2002)[DOI]
- Oh HC, "Hepatoprotective and free radical scavenging activities of prenylflavonoid, coumarin, and stilbene from Morus alba", Planta Medica, 68, pp.932~934 (2002)[DOI]
- Onogi A, "Flavonol glycoside from the leaves of Morus alba L", Shoyakugaku Zasshi, 47, pp.423~425 (1993)
- 박혜미, "추출방법에 따른 상지 추출물의 항산화 활성", 한국식품영양과학회지, 43(11), pp.1709~1715 (2014), 한국식품영양과학회
- 박진홍, "초음파 병행추출을 이용한 가시오갈피, 마황, 복분자 및 인진쑥의 항암활성 증진", 한국약용작물학회지, 12(4), pp.273~278 (2004), 한국약용작물학회
- Raven PH, "Biology of plants", pp.791~ (1992), Worth Publishers
- Ren K, "Role of interleukin-<TEX>$1{\beta}$<", TEX> during pain and inflammation, 2009, pp.60, 57~64
- Shin KH, "Studies on the chemical component and antioxidative effects of solanum lyratum", Korean Journal of Pharmacognosy, 26, pp.130~138 (1995)
- 신유현, "천연 에센셜 오일의 주요 구성물질 분석과 항산화 및 항균 효과에 관한 연구", 생명과학회지, 22(10), pp.1344~1351 (2012), 한국생명과학회[DOI]
- Simons RK, "Acyte lung injury in endotoxemic rats is associated with sustained circulating IL-6 levels and intrapulmonary CINC activity and neutrophil recruiment role of ciculating TNF-<TEX>${\alpha}$<", TEX> and IL-<TEX>$1{\beta}$<, Shock, pp.1996, 6[DOI]
- 송정수, "류마티스관절염 치료에서 종양괴사인자억제제 사용에 대한 고찰", 대한류마티스학회지, 14(1), pp.1~14 (2007), 대한류마티스학회
- Tanaka T, "IL-6 in inflammation, immunity, and disease", Cold Spring Harbor Perspectives in Biology, 6, pp.a016295~ (2014)[DOI]
- Wang MT, "Cyclooxygenase, prostanoid, and tumor progression", Cancer and Metastasis Reviews, 26, pp.525~534 (2007)[DOI]
- 우정향, "추출 방법에 따른 남구절초와 코스모스 추출물의 항산화 효과", 원예과학기술지, 28(3), pp.462~468 (2010), 한국원예학회
- 양선아, "산벚나무 부위별 추출물의 생리활성 비교", 한국약용작물학회지, 20(3), pp.159~164 (2012), 한국약용작물학회[DOI]
- Zamora R, "Inducible nitric oxide synthase and inflammatory diseases", Molecular Medicine, 6, pp.347~373 (2000)