감초와 상백피 복합추출물의 항염증 효과
Anti-inflammatory Effect of Complex Extracts from Glycyrrhiza glabra L. and Morus alba L.
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This study confirmed the anti-inflammatory effects of Glycyrrhiza glabra L. complexextracts (Glycyrrhiza glabra L. and Morus alba L.) by the complex ratio of Glycyrrhiza glabra L. and Morus alba L. extract. Cell viability was assessed using RAW 264.7 cells by MTT assay. TheGlycyrrhiza glabra L. complex extract was measured through changes in the levels ofprostaglandin E2 (PGE2), nitric oxide (NO), inflammatory cytokine (i.e., IL-1beta, IL-6, andTNF-alpha), COX-2, and iNOS in LPS-induced RAW 264.7 cells. All test results were analyzed byan ELISA reader, western blot analysis, and Luminex. Compared with those in the control group,all complex ratio extracts were not toxic below a concentration of 100 ㎍/㎖. In addition, thecomplex ratio extract treatments significantly reduced the productions of NO, PGE2, and cytokineand significantly inhibited the mRNA expression of COX-2 and iNOS in LPS-induced RAW 264.7cells. The results indicate that Glycyrrhiza glabra L. complex extracts prevented and alleviatedinflammatory indications. Thus, Glycyrrhiza glabra L. complex extracts may be developed as afunctional option material for feeding to improve diseases caused by inflammatory mediators.
Abstract
This study confirmed the anti-inflammatory effects of Glycyrrhiza glabra L. complexextracts (Glycyrrhiza glabra L. and Morus alba L.) by the complex ratio of Glycyrrhiza glabra L. and Morus alba L. extract. Cell viability was assessed using RAW 264.7 cells by MTT assay. TheGlycyrrhiza glabra L. complex extract was measured through changes in the levels ofprostaglandin E2 (PGE2), nitric oxide (NO), inflammatory cytokine (i.e., IL-1beta, IL-6, andTNF-alpha), COX-2, and iNOS in LPS-induced RAW 264.7 cells. All test results were analyzed byan ELISA reader, western blot analysis, and Luminex. Compared with those in the control group,all complex ratio extracts were not toxic below a concentration of 100 ㎍/㎖. In addition, thecomplex ratio extract treatments significantly reduced the productions of NO, PGE2, and cytokineand significantly inhibited the mRNA expression of COX-2 and iNOS in LPS-induced RAW 264.7cells. The results indicate that Glycyrrhiza glabra L. complex extracts prevented and alleviatedinflammatory indications. Thus, Glycyrrhiza glabra L. complex extracts may be developed as afunctional option material for feeding to improve diseases caused by inflammatory mediators.
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