국내산 벌개미취 잎 추출물의 α-glucosidase 억제능 및 항산화 활성 평가
Antioxidant and α-Glucosidase Inhibitory Activities of the Extracts of Aster koraiensis Leaves
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Background: The plant Aster koraiensis has long been used as an ingredient in folk medicine. It has been reported that Aster koraiensis extract (AKE) prevents the progression of diabetes-induced retinopathy and nephropathy. However, although these beneficial effects of AKE on diabetes complications have been identified, the antidiabetic effects of AKE have not yet been completely investigated and quantified. In the present study, the glucose-lowering and antioxidant effects of aqueous and ethanolic AKEs were evaluated. Methods and Results: The glucose-lowering effects of aqueous and ethanolic (30%−, 50%−, and 80%-ethanol) AKEs were investigated via α-glucosidase inhibitory assays. The mode of inhibition by AKEs on α-glucosidase was identified through kinetic analysis. The total antioxidant capacity of each of the 4 AKEs was evaluated by assessing their conversion rate of Cu2+ to Cu+. The content of chlorogenic acid and 3,5-di-O-caffeoylquinic acid, the bioactive compounds in AKE, in each extract were analyzed by high performance liquid chromatography (HPLC). The AKEs showed potent α-glucosidase inhibitory activity with mixed inhibition mode, and significant antioxidant capacity. Conclusions: These results of this study suggested that the AKEs tested had α-glucosidase inhibitory and antioxidant effects. Among the extracts, the 80% ethanol extract showed the most significant α-glucosidase inhibitory activity, with a half maximal inhibitory concentration (IC50 value) of 1.65 ± 0.36㎎/㎖ and a half maximal effective concentration (EC50 value) for its antioxidant activity of 0.42 ± 0.10㎎/㎖. It can therefore be used as a source of therapeutic agents to treat diabetes patients.
Abstract
Background: The plant Aster koraiensis has long been used as an ingredient in folk medicine. It has been reported that Aster koraiensis extract (AKE) prevents the progression of diabetes-induced retinopathy and nephropathy. However, although these beneficial effects of AKE on diabetes complications have been identified, the antidiabetic effects of AKE have not yet been completely investigated and quantified. In the present study, the glucose-lowering and antioxidant effects of aqueous and ethanolic AKEs were evaluated. Methods and Results: The glucose-lowering effects of aqueous and ethanolic (30%−, 50%−, and 80%-ethanol) AKEs were investigated via α-glucosidase inhibitory assays. The mode of inhibition by AKEs on α-glucosidase was identified through kinetic analysis. The total antioxidant capacity of each of the 4 AKEs was evaluated by assessing their conversion rate of Cu2+ to Cu+. The content of chlorogenic acid and 3,5-di-O-caffeoylquinic acid, the bioactive compounds in AKE, in each extract were analyzed by high performance liquid chromatography (HPLC). The AKEs showed potent α-glucosidase inhibitory activity with mixed inhibition mode, and significant antioxidant capacity. Conclusions: These results of this study suggested that the AKEs tested had α-glucosidase inhibitory and antioxidant effects. Among the extracts, the 80% ethanol extract showed the most significant α-glucosidase inhibitory activity, with a half maximal inhibitory concentration (IC50 value) of 1.65 ± 0.36㎎/㎖ and a half maximal effective concentration (EC50 value) for its antioxidant activity of 0.42 ± 0.10㎎/㎖. It can therefore be used as a source of therapeutic agents to treat diabetes patients.
참고문헌 (25)
- American Diabetes Association, "Diagnosis and classification of diabetes mellitus", Diabetes Care, 37(Supplement 1), pp.81~90 (2014)
- 최혜정, "번행초 추출물의 당뇨관련 효소에 관한 저해 활성", KSBB Journal, 23(5), pp.419~424 (2008), 한국생물공학회
- 최향숙, "수확 시기에 따른 벌개미취 정유의 주요 화합물 함량 변화", 한국식품영양학회지, 27(2), pp.194~202 (2014), 한국식품영양학회
- DiNicolantonio JJ, "Acarbose: Safe and effective for lowering postprandial hyperglycaemia and improving cardiovascular outcomes", Open Heart, 2, pp.e000327~ (2015)[DOI]
- Gerich J, "Pathogenesis and management of postprandial hyperglycemia: Role of incretin-based therapies", International Journal of General Medicine, 6, pp.877~895 (2013)
- Kalita D, "Inhibition of <TEX>${\alpha}$<", TEX>-glucosidase, <TEX>${\alpha}$<, PLoS One, pp.2018, 13[DOI]
- 김지은, "OLETF 당뇨모델동물을 이용한 맥문동 추출물(LP9M80-H)의 당뇨질환에 대한 효능", 생명과학회지, 22(5), pp.634~641 (2012), 한국생명과학회[DOI]
- Kim JH, "Aster koraiensis extract prevents diabetes-induced retinal vascular dysfunction in spontaneously diabetic Torii rats", BMC Complementary and Alternative Medicine, 17, pp.497~ (2017)
- Kim JH, "The extract of Aster koraiensis prevents retinal pericyte apoptosis in diabetic rats and its active compound, chlorogenic acid inhibits AGE formation and AGE", RAGE interaction, 2016, pp.8, E585~
- 김정현, "벌개미취 에탄올추출물의 STZ-유도 당뇨 모델에서의 최종당화산물의생성 및 교차결합에 미치는 효과", 생약학회지, 47(4), pp.312~318 (2016), 한국생약학회
- 김주성, "팔리다자주닭개비 잎 추출물 및 분획물의 항산화 및 α-글루코시다아제 저해 활성", 한국약용작물학회지, 24(3), pp.222~227 (2016), 한국약용작물학회[DOI]
- 고현민, "서양금혼초 부위별 추출물의 티로신에이즈, α-글루코시다아제 저해활성 및 항산화효과", 한국약용작물학회지, 25(3), pp.139~145 (2017), 한국약용작물학회[DOI]
- Lee GM, "New insight on development plan of herbal medicine resources production through the network of medicinal resources-related institutes in Korea", Korean Herbal Medicine Informatics, 6, pp.19~26 (2018)
- 이정민, "조각자 추출물의 항산화 활성 및 alpha-glucosidase 저해 활성", 생명과학회지, 21(1), pp.62~67 (2011), 한국생명과학회
- Lee SA, "In vitro assessment of selected Korean plants for antioxidant and antiacetylcholinesterase activities", Pharmaceutical Biology, 55, pp.2205~2210 (2017)
- Li X, "Antioxidant and cytoprotective effects of the di-O-caffeoylquinic acid family: The mechanism, structure-activity relationship, and conformational effect", Molecules, 23, pp.E222~ (2018)
- Matsui T, "Anti-hyperglycemic potential of natural products", Mini-Reviews in Medicinal Chemistry, 6, pp.349~356 (2006)
- Meng S, "Roles of chlorogenic acid on regulating glucose and lipids metabolism: A review", Evidence-Based Complementary and Alternative Medicine, 2013, pp.801457~ (2013)
- Oboh G, "Caffeic and chlorogenic acids inhibit key enzymes linked to type 2 diabetes(in vitro): A comparative study", Journal of Basic and Clinical Physiology and Pharmacology, 26, pp.165~170 (2015)
- Proenca C, "<TEX>${\alpha}$<", TEX>-Glucosidase inhibition by flavonoids: An in vitro and in silico structure-activity relationship study, 2017, pp.32, 1216~1228
- Rios JL, "Natural products for the treatment of type 2 diabetes mellitus", Planta Medica, 81, pp.975~994 (2015)
- 신언환, "벌개미취의 함유성분 분석과 항산화 활성", 한국식품영양과학회지, 43(1), pp.74~79 (2014), 한국식품영양과학회
- Global report on diabetes, "World Health Organization(WHO)", World Health Organization, 6~7 (2016)
- Yamagishi S, "Inhibition of postprandial hyperglycemia by acarbose is a promising therapeutic strategy for the treatment of patients with the metabolic syndrome", Medical Hypotheses, 65, pp.152~154 (2005)
- Yin Z, "<TEX>${\alpha}$<", TEX>-Glucosidase inhibitors isolated from medicinal plants, 2014, pp.3, 136~174