소리쟁이 뿌리로부터 세포독성물질 분리 및 정제
Purification and Identification of Cytotoxic Compounds from the Root of Rumex crispus L.
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Background: In the present study, we identified two cytotoxic compounds from the root of Rumex crispus L. using a bioassaybased method. Methods and Results: Compared with the other fractions, the diethyl ether (Et2O) fraction of R. crispus root extract exhibited the strongest of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging effect [scavenging concentration 50% (SC50) = 63.8 ± 1.47 ㎍/㎖], nitric oxide (NO) production inhibitory effect on the mouse macrophage cell line RAW264.7 [inhibitory concentration 50% (IC50) = 60.9 ± 7.52 ㎍/㎖] and cytotoxicity effect on the human hepatoma cell line, HepG2 [lethal concentration 50% (LC50) = 115.4 ± 1.86 ㎍/㎖]. According to the bioassay-based method, two cytotoxic compounds were purified from the Et2O fraction by using column chromatography and preparative high performance liquid chromatography (prep-HPLC). These two compounds were identified as parietin and chrysophanol by using nuclear magnetic resonance (NMR) and liquid chromatography quadruple time of flight mass spectrometry (LC-QTOF-MS). In addition, both parietin and chrysophanol exhibited a cytotoxicity effect on HepG2 cells, their LC50 values were 169.1 ± 17.67 μM and 111.5 ± 6.62 μM, respectively. Conclusions: Parietin and chrysophanol isolated from the Et2O fraction of the R. crispus root extract showed cytotoxicity in HepG2 cell.
Abstract
Background: In the present study, we identified two cytotoxic compounds from the root of Rumex crispus L. using a bioassaybased method. Methods and Results: Compared with the other fractions, the diethyl ether (Et2O) fraction of R. crispus root extract exhibited the strongest of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging effect [scavenging concentration 50% (SC50) = 63.8 ± 1.47 ㎍/㎖], nitric oxide (NO) production inhibitory effect on the mouse macrophage cell line RAW264.7 [inhibitory concentration 50% (IC50) = 60.9 ± 7.52 ㎍/㎖] and cytotoxicity effect on the human hepatoma cell line, HepG2 [lethal concentration 50% (LC50) = 115.4 ± 1.86 ㎍/㎖]. According to the bioassay-based method, two cytotoxic compounds were purified from the Et2O fraction by using column chromatography and preparative high performance liquid chromatography (prep-HPLC). These two compounds were identified as parietin and chrysophanol by using nuclear magnetic resonance (NMR) and liquid chromatography quadruple time of flight mass spectrometry (LC-QTOF-MS). In addition, both parietin and chrysophanol exhibited a cytotoxicity effect on HepG2 cells, their LC50 values were 169.1 ± 17.67 μM and 111.5 ± 6.62 μM, respectively. Conclusions: Parietin and chrysophanol isolated from the Et2O fraction of the R. crispus root extract showed cytotoxicity in HepG2 cell.
참고문헌 (36)
- Ahn SH, "Risk factors for hepatocellular carcinoma in Korea", Korean Journal of Medicine, 60, pp.123~130 (2001)
- Amano F, "Improved detection of nitric oxide radical(NO·) production in an activated macrophage culture with a radical scavenger, carboxy PTIO, and griess reagent", FEBS letters, 368, pp.425~428 (1995)
- Anagnostopoulou MA, "Radical scavenging activity of various extracts and fractions of sweet orange peel(Citrus sinensis)", Food Chemistry, 94, pp.19~25 (2006)
- 배기춘, "국내산 삼채 에탄올 추출물의 항염증 효과", 대한본초학회지, 27(6), pp.55~61 (2012), 대한본초학회
- 백설, "감송향의 in vitro 항산화 및 항염증 효과", 동의생리병리학회지, 23(4), pp.853~859 (2009), 한의병리학회
- Baig H, "In vitro evaluation of antioxidant properties of different solvent extracts of Rumex acetosella leaves", Oriental Journal of Chemistry, 27, pp.1509~1516 (2011)
- Bhandari M, "Extraction of anti-cancer water soluble compounds from Rumex crispus", The FASEB Journal, 29(supplement1), pp.897~ (2015)[DOI]
- Blois MS., "Antioxidant determinations by the use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Choi GJ, "Effects of chrysophanol, parietin, and nepodin of Rumex crispus on barley and cucumber powdery mildews", Crop Protection, 23, pp.1215~1221 (2004)
- Danielsen K, "NMR study of some anthraquinones from rhubarb", Magnetic Resonance in Chemistry, 30, pp.359~360 (1992)
- Elzaawely AA, "Antioxidant and antibacterial activities of Rumex japonicus HOUTT. aerial parts", Biological and Pharmaceutical Bulletin, 28, pp.2225~2230 (2005)
- Gautam R, "Anti-inflammatory, cyclooxygenase(COX)-2, COX-1 inhibitory, and free radical scavenging effects of Rumex nepalensis", Planta medica, 76, pp.1564~1569 (2010)
- Gerlier D, "Use of MTT colorimetric assay to measure cell activation", Journal of Immunological Methods, 94, pp.57~63 (1986)
- 황선우, "참소리쟁이 뿌리에서 안트라퀴논계 화합물의 분리 및 생리활성", Applied Biological Chemistry, 47(2), pp.274~278 (2004), 한국응용생명화학회
- 임남경, "Inhibitory Effect of the Leaves of Rumex crispus L. on LPS-induced Nitric Oxide Production and the Expression of iNOS and COX-2 in Macrophages", Natural Product Sciences, 20(1), pp.51~57 (2014), 한국생약학회
- 김보민, "HepG2 간암세포에서 미토콘드리아 경로를 통한 개똥쑥 추출물의 Apoptosis 유도 효과", 한국식품영양과학회지, 45(12), pp.1708~1716 (2016), 한국식품영양과학회
- 김은지, "Hep3B 간암세포에서 개똥쑥추출물로부터 Akt-mTOR-GSK3β 신호경로에 의한 apoptosis 효과", 생명과학회지, 26(7), pp.764~771 (2016), 한국생명과학회
- Ko SK, "General medicinal botany", pp.153~ (2009), Hakchangsa
- Krinsky NI., "Antioxidant functions of carotenoids", Free Radical Biology and Medicine, 7, pp.617~635 (1989)
- Lee NJ, "Antimutagenicity and cytotoxicity of the constituents from the aerial parts of Rumex acetosa", Biological and Pharmaceutical Bulletin, 28, pp.2158~2161 (2005)
- 이숙연, "소리쟁이 종자의 소염진통작용 및 간보호 효과", 생약학회지, 38(4), pp.334~338 (2007), 한국생약학회
- Lin R, "6-phosphogluconate dehydrogenase links oxidative PPP, lipogenesis and tumour growth by inhibiting LKB1-AMPK signaling", Nature Cell Biology, 17, pp.1484~1496 (2015)
- Lowenstein CJ, "Nitric oxide: A physiologic messenger", Annals of Internal Medicine, 120, pp.227~237 (1994)
- Lu CC, "Chrysophanol induces necrosis through the production of ROS and alteration of ATP levels in J5 human liver cancer cells", Molecular Nutrition and Food Research, 54, pp.967~976 (2010)
- Mulisa E, "Evaluation of wound healing and anti-inflammatory activity of the rhizomes of Rumex abyssinicus J.(polygonaceae) in mice", BMC Complementary and Alternative Medicine, 15, pp.341~ (2015)[DOI]
- 노종현, "노루오줌 에탄올 추출물의 산화스트레스 및 대장암 세포 억제활성", 한국약용작물학회지, 26(2), pp.141~147 (2018), 한국약용작물학회[DOI]
- Ni CH, "Chrysophanol-induced cell death(necrosis) in human lung cancer A549 cells is mediated through increasing reactive oxygen species and decreasing the level of mitochondrial membrane potential", Environmental Toxicology, 29, pp.740~749 (2014)
- 박의성, "Rumex crispus and Cordyceps militaris Mixture Ameliorates Production of Pro-Inflammatory Cytokines Induced by Lipopolysaccharide in C57BL", 6 Mice Splenocytes, 2018, pp.23(4), 374~381[DOI]
- Reuter S, "Oxidative stress, inflammation, and cancer: How are they linked?", Free Radical Biology and Medicine, 49, pp.1603~1616 (2010)
- 노숙령, "오미자 추출물의 간암세포 (SNU-398) 증식 억제 효과", Journal of Nutrition and Health, 35(2), pp.201~206 (2002), 한국영양학회
- Saunders IT, "Emodin inhibits colon cancer by modulating apoptotic and cell survival signals", American Association for Cancer Research, 78(supplement1) (2018)
- Sies H, "Lycopene: Antioxidant and biological effects and its bioavailability in the human", Proceedings of the Society for Experimental Biology and Medicine, 218, pp.121~124 (1998)
- Ueno Y, "Induction of apoptosis by T-2 toxin and other natural toxins in HL-60 human promyelotic leukemia cells", Journal of Natural Toxins, 3, pp.129~137 (1995)
- Wang W, "Antimicrobial activity and molecular docking studies of a novel anthraquinone from a marine-derived fungus Aspergillus versicolor", Natural Product Research, 32, pp.558~563 (2018)
- Xie QC, "Anti-proliferative of physcion 8-O-β-glucopyranoside isolated from Rumex japonicus Houtt. on A549 cell lines via inducing apoptosis and cell cycle arrest", BMC complementary and alternative medicine, 14, pp.377~ (2014)[DOI]
- Yıldırım A, "Determination of antioxidant and antimicrobial activities of Rumex crispus L. extracts", Journal of Agricultural and Food Chemistry, 49, pp.4083~4089 (2001)
