Effect of Staphylea bumalda Leaf Extracts on Macrophage Activation through Toll-Like Receptor 2 and Toll-Like Receptor 4-dependent c-Jun N-terminal Kinase Activation
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Background: The leaves of Staphylea bumalda (S. bumalda), a deciduous tree distributed in Korea, China, and Japan, are used to treat respiratory diseases or inflammation. However, the immune-enhancing activity of S. bumalda leaves has yet to be examined. Thus, we investigated the effect of water extracts of S. bumalda leaves (SBL) on macrophage activity using RAW264.7 mouse macrophage cells. Methods and Results: SBL increased the production of immunomodulators such as NO, iNOS, IL-1β, IL-6, TNF-α, and MCP-1 and activated the phagocytic activity of RAW264.7 cells. SBLmediated production of immunomodulators in RAW264.7 cells was blocked by inhibition of TLR2 and TLR4 and was attenuated by JNK inhibition. Moreover, SBL increased JNK phosphorylation, while inhibition of TLR2 and TLR4 blocked SBL-mediated JNK phosphorylation. These results suggest that SBL activates JNK through stimulating TLR2 and TLR4 in macrophages to induce immunomodulator production. In lipopolysaccharide-stimulated RAW264.7 cells, SBL inhibited the overproduction of immunomodulators. Conclusions: Given these results, SBL showed immunostimulatory activity under normal conditions and immunosuppressive activity during LPS-induced excessive immune response.
Abstract
Background: The leaves of Staphylea bumalda (S. bumalda), a deciduous tree distributed in Korea, China, and Japan, are used to treat respiratory diseases or inflammation. However, the immune-enhancing activity of S. bumalda leaves has yet to be examined. Thus, we investigated the effect of water extracts of S. bumalda leaves (SBL) on macrophage activity using RAW264.7 mouse macrophage cells. Methods and Results: SBL increased the production of immunomodulators such as NO, iNOS, IL-1β, IL-6, TNF-α, and MCP-1 and activated the phagocytic activity of RAW264.7 cells. SBLmediated production of immunomodulators in RAW264.7 cells was blocked by inhibition of TLR2 and TLR4 and was attenuated by JNK inhibition. Moreover, SBL increased JNK phosphorylation, while inhibition of TLR2 and TLR4 blocked SBL-mediated JNK phosphorylation. These results suggest that SBL activates JNK through stimulating TLR2 and TLR4 in macrophages to induce immunomodulator production. In lipopolysaccharide-stimulated RAW264.7 cells, SBL inhibited the overproduction of immunomodulators. Conclusions: Given these results, SBL showed immunostimulatory activity under normal conditions and immunosuppressive activity during LPS-induced excessive immune response.
참고문헌 (37)
- Bai Y, "Xinjiang herbal tea exerts immunomodulatory activity via TLR2", 4-mediated MAPK signaling pathways in RAW264. 7 cells and prevents cyclophosphamide-induced immunosuppression in mice, 2019, pp.228, 179~187
- Bordet E, "Macrophage-B cell interactions in the inverted porcine lymph node and their response to porcine reproductive and respiratory syndrome virus", Frontiers in Immunology, 10, pp.953~ (2019)[DOI]
- Cheng X, "Macrophage immunomodulatory activity of the polysaccharides from the roots of Bupleurum smithii var. parvifolium", Journal of Ethnopharmacology, 130, pp.363~368 (2010)
- Craxton A, "Macrophage-and dendritic cell-dependent regulation of human B-cell proliferation requires the TNF family ligand BAFF", Blood, 101, pp.4464~4471 (2003)
- Delgado AV, "Production of tumor necrosis factor-alpha, interleukin 1-beta, interleukin 2, and interleukin 6 by rat leukocyte subpopulations after exposure to substance P", Neuropeptides, 37, pp.355~361 (2003)
- Ding S, "Regulation of immune function by polyphenols", Journal of Immunology Research, 2018, pp.1264074~ (2018)[DOI]
- Geng L, "A heteropolysaccharide from Saccharina japonica with immunomodulatory effect on RAW264. 7 cells", Carbohydrate Polymers, 201, pp.557~565 (2018)
- Gong W, "Agronomic traits and molecular marker identification of wheat- Aegilops caudata addition lines", Frontiers in Plant Science, 8, pp.1743~ (2017)[DOI]
- Guerriero JL, "Macrophages : Their untold story in T cell activation and function", International Review of Cell and Molecular Biology, 342, pp.73~93 (2019)
- Haddad PS, "Natural health products, modulation of immune function and prevention of chronic diseases", Evidence-Based Complementary and Alternative Medicine, 2, pp.513~520 (2005)
- Hirayama D, "The phagocytic function of macrophage-enforcing innate immunity and tissue homeostasis", International Journal of Molecular Sciences, 19, pp.92~ (2018)[DOI]
- Hong SH, "The immune-enhancing activity of Cervus nippon mantchuricus extract(NGE)in RAW264. 7 macrophage cells and immunosuppressed mice", Food Research International, 99, pp.623~629 (2017)
- Huang D, "Absence of monocyte chemoattractant protein 1 in mice leads to decreased local macrophage recruitment and antigenspecific T helper cell type 1 immune response in experimental autoimmune encephalomyelitis", Journal of Experimental Medicine, 193, pp.713~726 (2001)
- Jeon JH, "The poly-γ-d-glutamic acid capsule surrogate of the Bacillus anthracis capsule is a novel toll-like receptor 2 agonist", Infection and Immunity, 83, pp.3847~3856 (2015)
- Kaneko A, "Glucuronides of phytoestrogen flavonoid enhance macrophage function via conversion to aglycones by β-glucuronidase in macrophages", Immunity, Inflammation and Disease, 5, pp.265~279 (2017)
- Kakutani R, "Essential role of Toll-like receptor 2 in macrophage activation by glycogen", Glycobiology, 22, pp.146~159 (2012)
- Kasimu R, "Water-soluble polysaccharide from Erythronium sibiricum bulb : Structural characterisation and immunomodulating activity", International Journal of Biological Macromolecules, 105, pp.452~462 (2017)
- Lapaque N, "Interactions between human NK cells and macrophages in response to Salmonella infection", Journal of Immunology, 182, pp.4339~4348 (2009)
- Lee JS, "Immunostimulating activity of the polysaccharides isolated from Cordyceps militaris", International Immunopharmacology, 11, pp.1226~1233 (2011)
- Li Q, "Role of toll-like receptor 4 in macrophage activation and tolerance during Salmonella enterica serovar Typhimurium infection", Infection and Immunity, 71, pp.4873~4882 (2003)
- Liu C, "Immunomodulatory activity of polysaccharide-protein complex from the mushroom sclerotia of Polyporus rhinocerus in murine macrophages", Journal of Agricultural and Food Chemistry, 64, pp.3206~3214 (2016)
- Li Y, "Immune regulation mechanism of Astragaloside IV on RAW264. 7 cells through activating the NF-κB", MAPK signaling pathway, 2017, pp.49, 38~49
- Lull C, "Anti-inflammatory and immunomodulating properties of fungal metabolites", Mediators of Inflammation, 2, pp.63~80 (2005)
- Mosser DM, "The many faces of macrophage activation", Journal of Leukocyte Biology, 73, pp.209~212 (2003)
- Nau GJ, "Human macrophage activation programs induced by bacterial pathogens", Proceedings of the National Academy of Sciences of the United States of America, 99, pp.1503~1508 (2002)
- Papayianni A, "Cytokines, growth factors, and other inflammatory mediators in glomerulonephritis", Renal Failure, 18, pp.725~740 (1996)
- Park YS, "Antioxidants and proteins in ethylene-treated kiwifruits", Food Chemistry, 107, pp.640~648 (2008)
- REYESALISHA WEHDNESDAY, "Tannic acid-mediated immune activation attenuates Brucella abortus infection in mice", Journal of Veterinary Science, 19(1), pp.51~57 (2018), 대한수의학회[DOI]
- Shao D, "Immunostimulatory and anti-inflammatory polysaccharides from Tripterygium wilfordii. : Comparison with organic extracts", Pharmaceutical Biology, 46, pp.8~15 (2008)
- Ting Shen, "Polysaccharide from wheat bran induces cytokine expression via the toll-like receptor 4-mediated p38 MAPK signaling pathway and prevents cyclophosphamide-induced immunosuppression in mice", Food & Nutrition Research, 61(1), pp.1344523~ (2017), SNF Swedish Nutrition Foundation[DOI]
- Taylor EL, "Nitric oxide : A key regulator of myeloid inflammatory cell apoptosis", Cell Death and Differentiation, 10, pp.418~430 (2003)
- Wang D, "Polysaccharide isolated from Sarcodon aspratus induces RAW264. 7 activity via TLR4-mediated NF-κB and MAPK signaling pathways", International Journal of Biological Macromolecules, 120, pp.1039~1047 (2018)
- We M, "Immunomodulatory properties of Grifola frondosa in submerged culture", Journal of Agricultural and Food Chemistry, 54, pp.2906~2914 (2006)
- Yang F, "A polysaccharide isolated from the fruits of Physalis alkekengi L. induces RAW264. 7 macrophages activation via TLR2 and TLR4-mediated MAPK and NF-κB signaling pathways", International Journal of Biological Macromolecules, 140, pp.895~906 (2019)
- Zhang M, "Immunomodulating activity of the polysaccharide TLH-3 from Tricholoma lobayense in RAW264. 7 macrophages", International Journal of Biological Macromolecules, 107, pp.2679~2268 (2017)
- Zheng-Xi Zhang, "New triterpenoids from Staphylea bumalda flower buds and their neuroprotective activity against H2O2-induced injury in vitro", Archives of Pharmacal Research, 41(12), pp.1162~1169 (2018), 대한약학회
- Zhou L, "Astragalus polysaccharides exerts immunomodulatory effects via TLR4-mediated MyD88-dependent signaling pathway in vitro and in vivo", Scientific Reports, 7, pp.44822~ (2017)[DOI]