Immunostimulatory Activity of Syneilesis palmata Leaves through Macrophage Activation and Macrophage Autophagy in Mouse Macrophages, RAW264.7 Cells
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Syneilesis palmata (SP) is a traditional medicinal plant. SP has been reported to have anti-inflammatory, anticancer, and anti-human immunodeficiency virus (HIV) activities. However, there is currently no research available on the immunostimulatory activity of SP. Therefore, in this study, we report that S. palmata leaves (SPL) activate macrophages. Increased secretion of both immunostimulatory mediators and phagocytic activity was observed in SPL-treated RAW264.7 cells. However, this effect was reversed by the inhibition of TLR2/4. In addition, inhibition of p38 decreased the secretion of immunostimulatory mediators induced by SPL, and inhibition of TLR2/4 decreased the phosphorylation of p38 induced by SPL. SPL augmented p62/SQSTM1 and LC3-II expression. The increase in protein levels of p62/ SQSTM1 and LC3-II induced by SPL was decreased by the inhibition of TLR2/4. The results obtained from this study suggest that SPL activates macrophages via TLR2/4-dependent p38 activation and induces autophagy in macrophages via TLR2/4 stimulation.
Abstract
Syneilesis palmata (SP) is a traditional medicinal plant. SP has been reported to have anti-inflammatory, anticancer, and anti-human immunodeficiency virus (HIV) activities. However, there is currently no research available on the immunostimulatory activity of SP. Therefore, in this study, we report that S. palmata leaves (SPL) activate macrophages. Increased secretion of both immunostimulatory mediators and phagocytic activity was observed in SPL-treated RAW264.7 cells. However, this effect was reversed by the inhibition of TLR2/4. In addition, inhibition of p38 decreased the secretion of immunostimulatory mediators induced by SPL, and inhibition of TLR2/4 decreased the phosphorylation of p38 induced by SPL. SPL augmented p62/SQSTM1 and LC3-II expression. The increase in protein levels of p62/ SQSTM1 and LC3-II induced by SPL was decreased by the inhibition of TLR2/4. The results obtained from this study suggest that SPL activates macrophages via TLR2/4-dependent p38 activation and induces autophagy in macrophages via TLR2/4 stimulation.
참고문헌 (26)
- Bartleson JM, "SARS-CoV-2, COVID-19 and the aging immune system", Nat. Aging, 1, pp.769~782 (2021)
- Li Q, "Immune response in COVID-19: what is next?", Cell Death Differ, 29, pp.1107~1122 (2022)
- Vishwakarma S, "Food nutrients as inherent sources of immunomodulation during COVID-19 pandemic", Lebensm. Wiss. Technol, 158, pp.113154~ (2022)
- Han JW, "Syneilesis palmata (Thunb.) Maxim. extract attenuates inflammatory responses via the regulation of TRIF-dependent signaling and inflammasome activation", J. Ethnopharmacol, 166, pp.1~4 (2015)
- 이규하, "Sesquiterpenes from Syneilesis palmata and Their Cytotoxicity Against Human Cancer Cell Lines In Vitro", Archives of Pharmacal Research, 28(3), pp.280~284 (2005), 대한약학회
- Heo BG, "Antiproliferative activity of Korean wild vegetables on different human tumor cell lines", Plant Foods Hum. Nutr, 64, pp.257~263 (2009)
- Min BS, "Inhibitory effects of Korean plants on HIV-1activities", Phytother. Res, 15, pp.481~486 (2001)
- Yi Y, "Structural features and immunomodulatory activities of polysaccharides of logan pulp", Carbohydr. Polym, 87, pp.636~643 (2012)
- Hirayama D, "The phagocytic function of macrophage-enforcing innate immunity and tissue homeostasis", Int. J. Mol. Sci, 19, pp.92~ (2018)
- Sieweke MH, "Beyond stem cells: self-renewal of differentiated macrophages", Science, 342, pp.1242974~ (2013)
- Wang YL, "Immunomodulatory effects of sinensetin on macrophage and Cyclophosphamide-induced Immunosuppression in mice", Die Pharmazie, 77, pp.147~151 (2022)
- Duque GA, "Macrophage cytokines: involvement in immunity and infectious diseases", Front. Immunol, 5, pp.491~ (2014)
- Mukherjee S, "TLR2 and TLR4 mediated host immune responses in major infectious diseases: a review", Braz. J. Infect. Dis, 20, pp.193~204 (2016)
- Iwasaki A, "Toll-like receptor control of the adaptive immune responses", Nat. Immunol, 5, pp.987~995 (2004)
- Kawai T, "Toll-like receptors and their crosstalk with other innate receptors in infection and immunity", Immunity, 34, pp.637~650 (2011)
- 박범석, "Recognition of lipopolysaccharide pattern by TLR4complexes", Experimental and Molecular Medicine, 45(12), pp.1~9 (2013), 생화학분자생물학회
- Medzhitov R, "TLR-mediated innate immune recognition", Semin. Immunol, 19, pp.1~2 (2007)
- Li Y, "Immune regulation mechanism of Astragaloside IV on RAW264.7 cells through activating the NF-κB", MAPK signaling pathway, 2017, pp.49, 38~49
- Wu MY, "Autophagy and macrophage functions: inflammatory response and phagocytosis", Cells, 9, pp.70~ (2020)
- Lee JC, "A protein kinase involved in the regulation of inflammatory cytokine biosynthesis", Nature, 372, pp.739~746 (1994)
- Nick JA, "Activation of a p38 mitogen-activated protein kinase in human neutrophils by lipopolysaccharide", J. Immunol, 156, pp.4867~4875 (1996)
- Rogov V, "Interactions between autophagy receptors and ubiquitin-like proteins form the molecular basis for selective autophagy", Mol. Cell, 53, pp.167~178 (2014)
- Yoshii SR, "Monitoring and measuring autophagy", Int. J. Mol. Sci, 18, pp.1865~ (2017)
- Andersen AN, "Coupling of HIV-1 antigen to the selective autophagy receptor SQSTM1", p62 promotes T-cell-mediated immunity, 2016, pp.7, 167~
- Xu Z, "Immunomodulatory mechanism of a purified polysaccharide isolated from Isaria cicadae Miquel on RAW264.7 cells via activating TLR4-MAPK-NF-κB signaling pathway", Int. J. Biol. Macromol, 164, pp.4329~4338 (2020)
- Chou YJ, "Vaccine adjuvant activity of a TLR4-activating synthetic glycolipid by promoting autophagy", Sci. Rep, 10, pp.8422~ (2020)