들깨 및 차조기 유전자원의 재분화능

Abstract

The establishment of an efficient protocol of plant regeneration from leaf explant cultures of Perilla spp. is reported. Regenerated shoots were obtained from leaf explant cultures on solid MS medium containing different concentrations of cytokinins and auxin. The effect of cytokonin and auxin differed depending on each acession. The combination treatments of high level of cytokinin and low level of auxin was more effective for plant regeneration in Perilla frutescens. The best concentration of sucrose was 3% for regeneration. Of spermidine, spermin and putrescine. treatments, the most effective treatment for plant regeneration was 10㎎/ℓ spermidine.