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논문

Selection of PCR Markers and Its Application for Distinguishing Dried Root of Three Species of Angelica

김동순|Jung Sook Sung|Kyong Hwan Bang|Dong Su In|Dong Hwi Kim|Hee Woon Park|Nak Sul Seong
한국약용작물학회|2005.04|한국약용작물학회지 (KOREAN JOURNAL OF MEDICINAL CROP SCIENCE)|제13권 제2호|pp. 121-125

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Abstract

An analysis of RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction) was performed with three Angelica species (A. gigas Nakai, A. sinensis (Olive.) Diels and A. acutiloba Kitag) in an effort to distinguish between members of these three species. Two arbitrary primers (OPC02, OPD11) out of 80 primers tested, produced 17 species-specific fragments among the three species. Eight fragments were specific for A. sinensis, four fragments specific for A. gigas, five specific for A. acutiloba. When primers OPC02 and OPD11 were used in the polymerase chain reaction, RAPD-PCR fragments that were specific for each of the three species were generated simultaneously. Primer OPC02 produced eight species-specific fragments: four were specific for A. sinensis, one for A. gigas, and three for A. acutiloba. Primer OPD11 produced nine species- specific fragments: four for A. sinensis, three for A. gigas, and two for A. acutiloba. The RAPD-PCR markers that were generated with these two primers should rapidly identify members of the three Angelica species. The consistency of the identifications made with these species-specific RAPD-PCR markers was demonstrated by the observation that each respective marker was generated from three accessions of each species, all with different origins. We also performed the RAPD-PCR analysis with the dried Angelica root samples that randomly collected from marketed and from the OPC02 primer, obtained a A. gigas- specific band and the band were cloned and sequenced.

참고문헌 (24)

  1. "Genetic diversity and relationships in mulberry as revealed by RAPD and ISSR marker assays" (2004)
  2. "Selection of RAPD marker to discriminate the bolting-resistant varieties and commercial dried medicinal materials of Angelica species", 10(1), pp.46~50 (2002)
  3. "Molecular distinction amongst varieties of Mulberry using RAPD and DAMD profiles", 1, pp.3~ (2001)
  4. "Chinese Medical Herbology and", 3, pp.918~924 (2004)
  5. "A rapid DNA isolation procedure for small quantities of fresh leaf tissue", pp.11~15 (1987)
  6. "Development of Species-Specific SCAR Markers in Bentgrass", 43 (2003)
  7. "Identification of red raspberry cultivars and an assessment to their relatedness using fingerprintings produced by random primers", pp.123~130 (1994)
  8. "The screening of Chinese traditional drugs by biological assay and the isolation of some active components International Journal of Chinese Medicine", pp.1~17 (1991)
  9. "Variation of RAPD patterns between male and female genomic DNAs in dioecious Rumex acetosa L", 16(1), pp.55~60 (2003)
  10. "Confirmation of QTL associated with common bacterial blight resistance in four different genetic backgrounds in common bean", 39, pp.1448~1455 (1999)
  11. "Molecular phylogeny of Gossypium species by DNA fingerprinting", 101, pp.931~938 (2000)
  12. "Isolation of molecular markers for tomato", pp.108~114 (1991)
  13. "RAPD fingerprinting of blackurrant cultivars", 90, pp.166~172 (1995)
  14. "Discrimination of the three Angelica species using the RAPDs and Internal Root Structure", 8(3), pp.243~249 (2000)
  15. "A novel method for screening species-specific gDNA probes for species identification", 32(4), pp.e45~ (2004)
  16. "Identification of DNA markers linked to the male sex in dioecious hemp", 98, pp.86~92 (1999)
  17. "Rapid identification of markers linked to a Pseudomonas resistance gene in tomato by using random primers and near-isogenetic lines", pp.2336~2340 (1991)
  18. "Identification of markers linked to disease resistance genes by bulked segregant analysis a rapid method to detect markers in specific genomic regions using segregating populations", pp.9828~88 9832 (1991)
  19. "The use of RAPD markers for identification of cultivated grapevine", 62, pp.237~243 (1995)
  20. "Identification of RFLP and RAPD markers linked to downy mildew resistance genes in lettuce by using near-isogenic lines", ge(me 34), pp.1021~1027 (1991)
  21. "Genetic Relationships among Orobanche Species as Revealed by RAPD Analysis", 91, pp.637~642 (2003)
  22. "Discrimination of Angelica Radix Based on anatomical characters", 12(1), pp.67~72 (2004)
  23. "Chemical and clinical evaluation of crude drugs derived from Angelica acutiloba and A", pp.201~208 (1985)
  24. "DNA polymorphisms amplified by arbitrary primers are useful as genetic markers", pp.6531~6535 (1990)

관련 논문AI추천

당귀 내추대성 품종 및 건재약재 판별을 위한 RAPD marker 선발구달회, 김홍식, 박상일, 박희운, 방경환, 성낙술, 유홍섭, 조준형한국약용작물학회 · 한국약용작물학회지2002 · 제10권 제1호A Study on the Discrimination of Angelica Species Roots by Dyeing서영남한국자원식물학회 · 한국자원식물학회지2007 · 제20권 제3호당귀(Angelica spp.)의 기원분석에 관한 분자생물학적 연구 현황 및 향후과제김윤희, 신의철, 이수진, 이신우, 조계만, 한은희한국식물생명공학회 · Journal of Plant Biotechnology2017 · 제44권 제1호진보된 DNA barcoding 기술을 이용한 당귀(Angelica)속 식물의 기원 판별 기술에 관한 연구 동향김윤희, 신용욱, 이신우한국식물생명공학회 · Journal of Plant Biotechnology2021 · 제48권 제3호Multiplex Polymerase Chain Reaction을 이용한 당귀 종 판별김용상, 김현규, 박혁주, 이동희한국약용작물학회 · 한국약용작물학회지2018 · 제26권 제1호Angelica 속 식물의 종판별을 위한 연구현황 및 전망이이, 길진수, 김성철, 김옥태, 김호방, 박상익, 엄유리, 정찬식, 차선우한국식물생명공학회 · Journal of Plant Biotechnology2016 · 제43권 제2호

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