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당귀(Angelica spp.)의 기원분석에 관한 분자생물학적 연구 현황 및 향후과제

Current status on the development of molecular markers for differentiation of the origin of Angelica spp.

이수진|김윤희|신의철|한은희|조계만|이신우
한국식물생명공학회 (Korean Society for Plant Biotechnology)|2017.03|Journal of Plant Biotechnology|제44권 제1호|pp. 12-18

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참고문헌

키워드

DNA barcodeAngelicamolecular markerorigin of species
주제분류농학

초록

The dried root of Angelica species is used in traditional Chinese medicine in East Asia, particularly in Korea, China and Japan. Since the plant origin differs in these countries, they are often misused or adulterated in the commercial markets, resulting in distrust among the consumers. Enormous efforts have therefore been focused to distinguish the origin for the Angelica genus, by using morphological or cytogenetical analyses, and chemical markers based on biochemical analyses of secondary metabolites. DNA is considerably stable against different cultivation conditions, and to treatment and processing after harvesting of plants. Hence, several researches have been filed for the development of molecular markers, based on the single nucleotide polymorphisms in specific regions of DNA. However, there are several obstacles for application in the commercial markets, concerning the reproducibility, accuracy, sensitivity, and rapidity of these tests. In this review, we summarize the research achievements that help classify the origin of Angelica species, in particular, Angelica gigas Nakai. A. sinensis(oliv.) Diels, A. acutiloba Kitag., and A. acutiloba var. sugiyamae Hikino. Further researches are required for practical applications.

Abstract

The dried root of Angelica species is used in traditional Chinese medicine in East Asia, particularly in Korea, China and Japan. Since the plant origin differs in these countries, they are often misused or adulterated in the commercial markets, resulting in distrust among the consumers. Enormous efforts have therefore been focused to distinguish the origin for the Angelica genus, by using morphological or cytogenetical analyses, and chemical markers based on biochemical analyses of secondary metabolites. DNA is considerably stable against different cultivation conditions, and to treatment and processing after harvesting of plants. Hence, several researches have been filed for the development of molecular markers, based on the single nucleotide polymorphisms in specific regions of DNA. However, there are several obstacles for application in the commercial markets, concerning the reproducibility, accuracy, sensitivity, and rapidity of these tests. In this review, we summarize the research achievements that help classify the origin of Angelica species, in particular, Angelica gigas Nakai. A. sinensis(oliv.) Diels, A. acutiloba Kitag., and A. acutiloba var. sugiyamae Hikino. Further researches are required for practical applications.

참고문헌 (37)

  1. 방경환, "당귀 내추대성 품종 및 건재약재 판별을 위한 RAPD marker 선발", 한국약용작물학회지, 10(1), pp.46~50 (2002), 한국약용작물학회
  2. Chan PH, "Metabonomic analysis of water extracts from different angelica roots by ¹H-nuclear magnetic resonance spectroscopy", Molecules, 19, pp.3460~3470 (2014)
  3. 조창희, "근적외선분광법, 전자코 및 엑스 선형광법을 이용한 당귀의 기원판별법 비교 연구", 약 학 회 지, 46(3), pp.161~167 (2002), 대한약학회
  4. 방재옥, "Angelica속 식물 7종의 세포유전학적 분석", 한국약용작물학회지, 13(3), pp.118~121 (2005), 한국약용작물학회
  5. 최해운, "Molecular and Cytological Characterization of Species-Specific Repetitive DNA Sequences for Angelica acutiloba", Genes & Genomics, 29(4), pp.503~511 (2007), 한국유전학회
  6. 최호영, "當歸類 한약재 감별을 위한 ITS 부위의 염기서열 및 AFLP 분석", 대한본초학회지, 19(2), pp.91~100 (2004), 대한본초학회
  7. Feng T, "Molecular systematics of Angelica and allied genera (Apiaceae) from the Hengduan Mountains of China based on nrDNA ITS sequences: phylogenetic affinities and biogeographic implications", J Plant Res, 122, pp.403~414 (2009)
  8. Han GQ, "The screening of Chinese traditional drugs by biological assay and the isolation of some active components", Int J Chi Med, 16, pp.1~17 (1991)
  9. Hosokawa K1, "The sequences of the spacer region between the atpF and atpA genes in the plastid genome allows discrimination among three varieties of medicinal Angelica", Planta Med, 72, pp.570~571 (2006)
  10. Jeong SY, "Quantitative analysis of marker compounds in Angelica gigas, Angelica sinensis, and Angelica acutiloba by HPLC", DAD, 2015, pp.63, 504~511
  11. 김수언, "Determination of Biosynthetic Pathway of Decursin in Hairy Root Culture of Angelica gigas", Applied Biological Chemistry, 51(4), pp.258~262 (2008), 한국응용생명화학회
  12. 김동순, "Selection of PCR Markers and Its Application for Distinguishing Dried Root of Three Species of Angelica", 한국약용작물학회지, 13(2), pp.121~125 (2005), 한국약용작물학회
  13. Katoh A1, "Systems biology in a commercial quality study of the Japanese Angelica radix: toward an understanding of traditional medicinal plants", Am J Chin Med, 39, pp.757~777 (2011)
  14. 김혜진, "Chemotype Discrimination and Rapid Identification of Angelica Roots by DART-TOF-MS", Natural Product Sciences, 17(3), pp.202~205 (2011), 한국생약학회
  15. 김정률, "UHPLC-DAD 및 다변량분석법을 이용한 참당귀의 산지감별법 연구", 생약학회지, 44(4), pp.332~335 (2013), 한국생약학회
  16. 김영화, "미각센서와 DNA 염기서열을 이용한 당귀류 비교", 대한본초학회지, 27(6), pp.37~42 (2012), 대한본초학회
  17. Kobayashi S, "Gas chromatography-massspectrometry based metabolic profiling for the identification of discrimination markers of Angelicae Radix and its application to gas chromatography-flame ionization detector system", J Biosci Bioeng, 114, pp.232~236 (2012)
  18. 이병윤, "The taxonomic status of Angelica purpuraefolia and its allies in Korea : Inferences based on ITS molecular phylogenetic analyses", 식물분류학회지, 41(3), pp.209~214 (2011), 한국식물분류학회
  19. Lee, "Discrimination of the three Angelica species using the RAPDs and internal root structure", Kor J Med Crop Sci, 8, pp.243~249 (2000)
  20. Liu J, "A rapid discrimination of authentic and unauthentic Radix Angelicae Sinensis growth regions by electronic nose coupled with multivariate statistical analyses", Sensors (Basel), 14, pp.20134~20148 (2014)
  21. Lu GH, "Quantification of ligustilides in the roots of Angelica sinensis and related umbelliferous medicinal plants by high-performance liquid chromatography and liquid chromatography-mass spectrometry", J Chromatogr A, 1046, pp.101~107 (2004)
  22. Lu GH, "Development of high-performance liquid chromatographic fingerprints for distinguishing Chinese Angelica from related umbelliferae herbs", J Chromatogr A, 1073, pp.383~392 (2005)
  23. Mizukami H, "Amplification and sequence of a 5s-rRNA gene spacer region from the crude drug "angelica root"", Biol Pharm Bull, 18, pp.1299~1301 (1995)
  24. 박상익, "당귀 종판별을 위한 엽록체 기반 SSR 마커 개발", 한국약용작물학회지, 24(4), pp.317~322 (2016), 한국약용작물학회[DOI]
  25. 박완수, "한국 당귀, 중국 당귀, 일본 당귀의 생체광자(Biophoton) 방출 비교 연구", 대한본초학회지, 22(4), pp.95~100 (2007), 대한본초학회
  26. Piao XL, "Development of gas chromatographic", mass spectrometry-pattern recognition method for the quality control of Korean Angelica, 2007, pp.44(5), 1163~1167
  27. Jung-Chul Seo, "Identification of Angelica Species by Pyrosequencing", 대한한의학회지, 25(4), pp.147~151 (2004), 대한한의학회
  28. 서영남, "A Study on the Discrimination of Angelica Species Roots by Dyeing", 한국자원식물학회지, 20(3), pp.247~250 (2007), 한국자원식물학회
  29. 성정숙, "당귀의 해부형태학적 특징에 따른 기원판별", 한국약용작물학회지, 12(1), pp.67~72 (2004), 한국약용작물학회
  30. Tarachiwin L, "Fukusaki E(2008)Quality evaluation of Angelica acutiloba Kitagawa roots by 1H NMR-based metabolic fingerprinting", J Pharm Biomed Anal, 48, pp.42~48 (2008)
  31. Terasawa K, "Chemical and clinical evaluation of crude drugs derived from Angelica acutiloba and A. sinensis", Fitoterapia, 56, pp.201~208 (1985)
  32. Tianniam S, "Non-targeted metabolite fingerprinting of oriental folk medicine Angelica acutiloba roots by ultra performance liquid chromatography timeof-flight mass spectrometry", J Sep Sci, 32, pp.2233~2244 (2009)
  33. Tianniam S, "Pyrolysis GC-MS-based metabolite fingerprinting for quality evaluation of commercial Angelica acutiloba roots", J Biosci Bioeng, 109, pp.89~93 (2010)
  34. Tianniam S, "Metabolic profiling of Angelica acutiloba roots utilizing gas chromatography-time-of-flight-mass spectrometry for quality assessment based on cultivation area and cultivar via multivariate pattern recognition", J Biosci Bioeng, 105, pp.655~659 (2008)
  35. Wang X, "A nucleotide signature for the identification of Angelicae Sinensis Radix (Danggui) and its products", Sci. Rep, 6, pp.34940~ (2016)[DOI]
  36. Yuan QJ, "Identification of species and materia medica within Angelica L. (Umbelliferae) based on phylogeny inferred from DNA barcodes", Mol Ecol Resour, 15, pp.358~371 (2014)
  37. Zhao KJ, "Molecular genetic and chemical assessment of radix Angelica (Danggui) in China", J Agric Food Chem, 51(9), pp.2576~2583 (2003)

관련 논문AI추천

Angelica 속 식물의 종판별을 위한 연구현황 및 전망이이, 길진수, 김성철, 김옥태, 김호방, 박상익, 엄유리, 정찬식, 차선우한국식물생명공학회 · Journal of Plant Biotechnology2016 · 제43권 제2호진보된 DNA barcoding 기술을 이용한 당귀(Angelica)속 식물의 기원 판별 기술에 관한 연구 동향김윤희, 신용욱, 이신우한국식물생명공학회 · Journal of Plant Biotechnology2021 · 제48권 제3호엽록체기반 SSR marker를 이용한 당귀의 기원 판별이이, 정희, 구성철, 길진수, 김성철, 김옥태, 김호방, 박상익, 엄유리, 황보경한국약용작물학회 · 한국약용작물학회지2017 · 제25권 제6호Multiplex Polymerase Chain Reaction을 이용한 당귀 종 판별김용상, 김현규, 박혁주, 이동희한국약용작물학회 · 한국약용작물학회지2018 · 제26권 제1호ITS 영역의 HRM 분석을 통한 참당귀(Angelica gigas Nakai)의 특이적 SNP 분자표지 개발김윤희, 신용욱, 이수진, 이신우, 한은희한국식물생명공학회 · Journal of Plant Biotechnology2021 · 제48권 제2호세발당귀(Angelica gigas Jiri)의 판별을 위한 ARMS-PCR용 분자표지 개발김윤희, 신용욱, 이수진, 이신우, 한은희한국식물생명공학회 · Journal of Plant Biotechnology2021 · 제48권 제1호

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