HPLC의 이동상 용매조건에 따른 인삼 Ginsenoside 분석
Analytical Optimum of Ginsenosides according to the Gradient Elution of Mobile Phase in High Performance Liquid Chromatography
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조회
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다운로드
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참고문헌
키워드
초록
This study was conducted to analyze not only for the quality guaranteed of red ginseng but also for the minor ginsenosides. Although several studies have reported to analyze ginseng saponins, those were focused to major saponins, including 6 to 7 ginsenosides. As increase of interest in medicinal effect of ginseng products, anasis of various ginsenosides in both red and white ginseng are strongly demanded. To perform optital condition of 12 ginsenoside analysis, We controlled HPLC conditions, such as the gradient elution of the mobile phase. We found the adequate separation method for 12 ginsenosides. The optimum condition was as following : H2O/CH3CN ratios were 82/18, 70/30, 55/45 and 50/50, respectively. Solvent flow rate was 1.00 ㎖/min. Column temperature was kept to 35℃. UV detector was set to 203 ㎚.
Abstract
This study was conducted to analyze not only for the quality guaranteed of red ginseng but also for the minor ginsenosides. Although several studies have reported to analyze ginseng saponins, those were focused to major saponins, including 6 to 7 ginsenosides. As increase of interest in medicinal effect of ginseng products, anasis of various ginsenosides in both red and white ginseng are strongly demanded. To perform optital condition of 12 ginsenoside analysis, We controlled HPLC conditions, such as the gradient elution of the mobile phase. We found the adequate separation method for 12 ginsenosides. The optimum condition was as following : H2O/CH3CN ratios were 82/18, 70/30, 55/45 and 50/50, respectively. Solvent flow rate was 1.00 ㎖/min. Column temperature was kept to 35℃. UV detector was set to 203 ㎚.
참고문헌 (16)
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