인삼·산양삼·자연산 산삼의 ginsenoside 함량 분석 및 홍삼화 후의 변화 관찰
Component analysis of cultivated ginseng, cultivated wild ginseng, and wild ginseng and the change of ginsenoside components in the process of red ginseng.
2
조회
0
다운로드
17
인용
0
참고문헌
키워드
초록
Objectives: The aim of this experiment is to provide an objective differentiation of cultivated ginseng, cultivated wild ginseng, and wild ginseng through component analysis, and to know the change of ginsenoside components in the process for making red ginseng. Methods: Comparative analysis of ginsenoside Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg3, Rh1 and Rh2 from the cultivated ginseng 4 and 6 years, cultivated wild ginseng, and wild ginseng were conducted using High Performance Liquid Chromatography(hereafter HPLC). And the same analyses were conducted in the process of red ginseng. Results:1. For content comparison of ginsenoside Rb1, Rc, Rd, Rf, Rg1 and Rh1, wild ginseng showed high content, followed cultivated ginseng 4 and 6 years, cultivated wild ginseng showed low content than any other samples. 2. For content comparison of ginsenoside Rb2 and Re, cultivated ginseng 4 years showed high content,followed wild ginseng and cultivated ginseng 6 years, cultivated wild ginseng showed low content than any other samples. 3. For content comparison of ginsenoside Rg3, wild ginseng and cultivated wild ginseng were only showed low content. 4. For content comparison of ginsenoside Rh2, cultivated wild ginseng was only showed low content. 5. In the process of red ginseng, ginsenoside Rb1, Rb2, Rc, Rd, Rg3 and Rh1 were increased, and ginsenoside Re and Rg1 were decreased in cultivated wild ginseng. 6. In the process of red ginseng, ginsenoside Rg3 and Rh1 were increased, and ginsenoside Rb2, Rc, and Re were decreased in cultivated ginseng 4 years. 7. In the process of red ginseng, ginsenoside Rb1, Rb2, Rf and Rh1 were increased, and ginsenoside Rc and Rd were decreased in cultivated ginseng 6 years. Conclusions: Distribution of ginsenoside contents to the cultivated ginseng, cultivated wild ginseng, and wild ginseng was similar and was not showed special characteristics between samples. And the change of ginsenoside to the process of red ginseng, cultivated ginseng and cultivated wild ginseng were showed different aspect.
Abstract
Objectives: The aim of this experiment is to provide an objective differentiation of cultivated ginseng, cultivated wild ginseng, and wild ginseng through component analysis, and to know the change of ginsenoside components in the process for making red ginseng. Methods: Comparative analysis of ginsenoside Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg3, Rh1 and Rh2 from the cultivated ginseng 4 and 6 years, cultivated wild ginseng, and wild ginseng were conducted using High Performance Liquid Chromatography(hereafter HPLC). And the same analyses were conducted in the process of red ginseng. Results:1. For content comparison of ginsenoside Rb1, Rc, Rd, Rf, Rg1 and Rh1, wild ginseng showed high content, followed cultivated ginseng 4 and 6 years, cultivated wild ginseng showed low content than any other samples. 2. For content comparison of ginsenoside Rb2 and Re, cultivated ginseng 4 years showed high content,followed wild ginseng and cultivated ginseng 6 years, cultivated wild ginseng showed low content than any other samples. 3. For content comparison of ginsenoside Rg3, wild ginseng and cultivated wild ginseng were only showed low content. 4. For content comparison of ginsenoside Rh2, cultivated wild ginseng was only showed low content. 5. In the process of red ginseng, ginsenoside Rb1, Rb2, Rc, Rd, Rg3 and Rh1 were increased, and ginsenoside Re and Rg1 were decreased in cultivated wild ginseng. 6. In the process of red ginseng, ginsenoside Rg3 and Rh1 were increased, and ginsenoside Rb2, Rc, and Re were decreased in cultivated ginseng 4 years. 7. In the process of red ginseng, ginsenoside Rb1, Rb2, Rf and Rh1 were increased, and ginsenoside Rc and Rd were decreased in cultivated ginseng 6 years. Conclusions: Distribution of ginsenoside contents to the cultivated ginseng, cultivated wild ginseng, and wild ginseng was similar and was not showed special characteristics between samples. And the change of ginsenoside to the process of red ginseng, cultivated ginseng and cultivated wild ginseng were showed different aspect.
참고문헌 (27)
- Shin SS, "Critic standardization and object ivity of mountain grown ginseng", KIOM, 5, pp.107~114 (2001)
- School of Korean Medicine. Dept. of Herbology, "Herbology", Youngrim, 531~ (1994)
- Brekhman. II, "Panax ginseng", Leningard, 1~5 (1957)
- 고려인삼학회, "고려삼의 이해", Advances in Ginseng Research, 127~137 (1998)
- 최진호, "인삼의 신비", pp.13~14 (1984), 교문사
- Nam KY, "최신 고려인삼", 천일인쇄소, 56~ (1996)
- Oliveira, ACC, "Protective effects of Panax ginseng on muscle injury and inflammation after eccentric exercise", Comparative Biochemistry and Physiology Part C, 130, pp.369~377 (2001)
- Kim, JS, "Screening of anti-oxidative components from red ginseng saponin", Korean J. Ginseng Sci., 20, pp.173~178 (1996)
- Kim, MJ, "The effects of ginseng saponin on the mouse immune system", Korean J. Ginseng Sci., 11, pp.130~135 (1987)
- 0Ha TY, "Inhibitory effects of Panax ginseng on tumorigenesis in mice", J Cheonbuk medical Sci., 11(11), pp.1~11 (1987)
- Shibata, S, "Studies on saponins and sapogenins of ginseng. The structure of panaxatriol", Tetrahedron Lett., 3, pp.207~213 (1965)
- Park JD, "Rescent studies on the chemical constituents of Korean Ginseng", Korean J. Ginseng Sci., 20(4), pp.389~415 (1996)
- 정춘식, "흰쥐의 염산·에탄올 유발 위염 위조직에서 ginsenoside Rb1의 항산화 효과", 생약학회지, 33(3), pp.252~256 (2002), 한국생약학회
- Choi KJ, "The constituent of material ginseng and management of quality", Korean J Ginseng Sci., 15(3), pp.247~256 (1991)
- Hideo H, "Metabolites of ginseng saponin by enterobacteria and anti cancer substance include it's useful constituent", Korea Plant., (10), pp.164266~ (1998)
- Seong-SooShoi, "Effect of Ginsenoside Rd on Nitric Oxide System Induced by Lipopolysaccharide Plus TNF-α in C6 Rat Glioma Cells", Archives of Pharmacal Research, 26(5), pp.375~382 (2003), 대한약학회
- Yokozawa T, "The role of ginsenoside Rd in cisplatin-induces acute renal failure", Ren. Fail., 22, pp.115~127 (2000)
- Wang W, "In vitro anti-cancer activity and structure-activity relationships of natural products isolated from fruits of Panax ginseng", Cancer Chemoth Pharmacol., 59, pp.589~601 (2007)
- LY Wu J, "Inhibitory effect of 20(S)-ginsenoside Rg_3 on growth and metasis of Lewis pulmonary carcinoma", Zhonggliu Fangzhi Yanjiu, 33, pp.311~313 (2006)
- David GP, "Ginsenosides 20(S)-protopanaxadiol and Rh2 reduce cell proliferation and increase sub-G1 cells in two cultured intestinal cell lines, Int-407 and Caco-2", Can. J Physiol. Pharmacol., 82, pp.183~190 (2004)
- Morita, T, "Chemical studies on Panax genus plants grown in Asia", 박사, pp.1986
- Attele AS, "Ginseng pharmacology: multiple constituents and mutiple actions", Biochem. Pharmacol., 58, pp.1685~1693 (1999)
- 이상인, "본초학", pp.60~ (1985), 수서원
- Fuzzati, N, "Liquid chromatographyelectrospray mass spectrometric identification of ginsenosides in Panax ginseng roots", J. Chromatogr., 854, pp.69~79 (1999)
- Wang, H, "Determination of ginsenoside Rg3 in plasma by solid-phase extraction and high-performance liquid chromatography for pharmaco-kinetic study", J. Chromatogr., 731, pp.403~409 (1999)
- Kwon, S. W, "Liquid chromatographic determination of less polar ginsenosides in processed ginseng", J. Chromatogr., 921, pp.335~339 (2001)
- Ko, S. R., "Content and composition of saponin compounds of Panax species", Korean J. Ginseng Sci., 19, pp.254~295 (1995)
