털부처꽃 뿌리로부터 조제된 분획물의 생리활성
Biological Activity of Organic Solvent Fractions from Lythrum salicaria L. (Root)
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Root extract of Lythrum salicaria reported a hepato-protective effect on CCl4-induced liver toxicity of rat was prepared into fractions such as n-hexane up layer (HA), n-hexane down layer (HB), diethyl ether (E), ethylacetate (EA), nbutanol (B) and water (W). Fractions prepared were tested their activities in vitro and in vivo condition. All of the fractions showed effective antioxidant asctivities on DPPH radical and CuSO4-induced oxidation of human low density lipoprotein and E fraction showed the highest inhibitory effect (98.1% at 50 ㎍/㎖) on linoleic acid autoxidation at 40℃, which was more effective than α-tocopherol (82.4%). Five fractions (H = HA plus HB, E, EA, B, and W, 150 ㎎/㎏/day) were fed into Sprague Dawley, male rats for 4 days, which were intoxicated with intra-peritoneal injection of carbon tetrachloride (1 ㎖/㎏ in corn oil) at the 4th day and were sacrificed in 24 hrs. Serum tumor necrosis factor-alpha (TNF-α), a proinflammatory cytokine, elevated with CCl4-intoxication in negative control group (83 pg/㎖) was significantly decreased in E fraction-supplemented group (18 pg/㎖). Cu, Zn-superoxide dismutase (SOD) activity increased in negative control group (0.12 U/㎎ protein) was decreased in E fraction (0.07 U/㎎ protein). From the results, it is suggested that ether fraction from root extract of L. salicaria would be a potent antioxidant candidate for ameliorating liver injury induced by chemical intoxicant.
Abstract
Root extract of Lythrum salicaria reported a hepato-protective effect on CCl4-induced liver toxicity of rat was prepared into fractions such as n-hexane up layer (HA), n-hexane down layer (HB), diethyl ether (E), ethylacetate (EA), nbutanol (B) and water (W). Fractions prepared were tested their activities in vitro and in vivo condition. All of the fractions showed effective antioxidant asctivities on DPPH radical and CuSO4-induced oxidation of human low density lipoprotein and E fraction showed the highest inhibitory effect (98.1% at 50 ㎍/㎖) on linoleic acid autoxidation at 40℃, which was more effective than α-tocopherol (82.4%). Five fractions (H = HA plus HB, E, EA, B, and W, 150 ㎎/㎏/day) were fed into Sprague Dawley, male rats for 4 days, which were intoxicated with intra-peritoneal injection of carbon tetrachloride (1 ㎖/㎏ in corn oil) at the 4th day and were sacrificed in 24 hrs. Serum tumor necrosis factor-alpha (TNF-α), a proinflammatory cytokine, elevated with CCl4-intoxication in negative control group (83 pg/㎖) was significantly decreased in E fraction-supplemented group (18 pg/㎖). Cu, Zn-superoxide dismutase (SOD) activity increased in negative control group (0.12 U/㎎ protein) was decreased in E fraction (0.07 U/㎎ protein). From the results, it is suggested that ether fraction from root extract of L. salicaria would be a potent antioxidant candidate for ameliorating liver injury induced by chemical intoxicant.
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