땃두릅나무 재분화 유식물체의 순화 체계 및 생육 특성
Establishment of Acclimatization System and Growth Characteristics for Regenerated Plants of Oplopanax elatus Nakai
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Background: Oplopanax elatus is widely distributed at high altitudes (about 1,100 m) in China, Russia and Korea. It is hard to propagate, breed, and difficult to grow. Hence, it has been designated as a rare and endangered medicinal plant. A study was conducted to establish a system for large scale seedling production of Oplopanax elatus in vitro and to find the ideal environment for its seedling growth. Methods and Results: In this study, the explants produced under in vitro conditions during our previous study were grouped into three categories (under 10 ㎜, 10 ㎜ - 30 ㎜ and above 30 ㎜) based on plant height and were transferred to the growth-chamber and greenhouse for two weeks in each setting for acclimatization. The plantlet category of above 30 ㎜ showed good performance, and was further evaluated under three acclimatization methods as follows: three different growth media (commercial soil, commercial soil + perlite, commercial soil + sand), four shading levels (0%, 50%, 70%, 90%) and four altitude levels (157 m, 218 m, 601 m, 870 m) in Gangwon province of South Korea. As results, O. elatus seedlings showed better growth characteristics at 870 m of altitude, 70% shading level and in the commercial soil compared to other treatments. Conclusions: The regenerated seedlings of Oplopanax elatus obtained through plant tissue culture would be advantageous for use in large scale seedling production systems paired with a good acclimation method. For obtaining optimal results, it is recommended that seedling be acclimatized in a high altitude environment.
Abstract
Background: Oplopanax elatus is widely distributed at high altitudes (about 1,100 m) in China, Russia and Korea. It is hard to propagate, breed, and difficult to grow. Hence, it has been designated as a rare and endangered medicinal plant. A study was conducted to establish a system for large scale seedling production of Oplopanax elatus in vitro and to find the ideal environment for its seedling growth. Methods and Results: In this study, the explants produced under in vitro conditions during our previous study were grouped into three categories (under 10 ㎜, 10 ㎜ - 30 ㎜ and above 30 ㎜) based on plant height and were transferred to the growth-chamber and greenhouse for two weeks in each setting for acclimatization. The plantlet category of above 30 ㎜ showed good performance, and was further evaluated under three acclimatization methods as follows: three different growth media (commercial soil, commercial soil + perlite, commercial soil + sand), four shading levels (0%, 50%, 70%, 90%) and four altitude levels (157 m, 218 m, 601 m, 870 m) in Gangwon province of South Korea. As results, O. elatus seedlings showed better growth characteristics at 870 m of altitude, 70% shading level and in the commercial soil compared to other treatments. Conclusions: The regenerated seedlings of Oplopanax elatus obtained through plant tissue culture would be advantageous for use in large scale seedling production systems paired with a good acclimation method. For obtaining optimal results, it is recommended that seedling be acclimatized in a high altitude environment.
참고문헌 (24)
- 변효증, "공정육묘시 재활용 상토에 신규상토 및 펄라이트의 혼합비율이 상토의 이화학적 특성과 과채류 묘소질에 미치는 영향", 생물환경조절학회지, 21(3), pp.213~219 (2012), (사) 한국생물환경조절학회
- "Tissue culture and ex-vitro acclimatization of Rhodedendron sp" (2007)[URL]
- Fu LK., "China plant red data book: Rare and endangered plants", pp.741~789 (1992), Science Press
- Fuchigami LH, "Abaxial transpiration and water loss in aseptically cultured plum", Journal of the American Society for Horticultural Science, 106, pp.519~522 (1981)
- 한봉희, "분화용 Anthurium andreanum 'Atlanta'의 기내번식", Journal of Plant Biotechnology, 30(2), pp.179~184 (2003), 한국식물생명공학회
- 한봉희, "Alocasia cadieri Chantrier의 기내번식", Journal of Plant Biotechnology, 31(1), pp.61~65 (2004), 한국식물생명공학회
- Huang W, "Quantitative analysis of six polyynes and one polyene in Oplopanax horridus and Oplopanax elatus by pressurized liquid extraction and on-line SPE-HPLC", Journal of Pharmaceutical and Biomedical Analysis, 53, pp.906~910 (2010)
- 김현진, "차광 정도와 삽목용토가 골담초와 애기기린초의 삽수 생육에 미치는 영향", 한국약용작물학회지, 23(4), pp.271~276 (2015), 한국약용작물학회[DOI]
- 김희영, "현탁배양을 통한 땃두릅나무의 체세포배 유도", 한국약용작물학회지, 20(6), pp.461~465 (2012), 한국약용작물학회[DOI]
- 김윤희, "하월시아 옥선(Haworthia truncata)의 기내 대량 증식 및 순화 조건 구명", Journal of Plant Biotechnology, 46(2), pp.127~135 (2019), 한국식물생명공학회[DOI]
- Kozai T., "Environmental control in photosutotrphic plant tissue culture", Environmental Control Biology, 30, pp.193~197 (1992)
- 이병국, "갯방풍(Glehnia littoralis Schmidt et Miquiel)에서 캘러스 유도 및 식물체 재분화에 미치는 생장조절제의 효과", 한국자원식물학회지, 17(2), pp.153~160 (2004), 한국자원식물학회
- 이경철, "수분스트레스가 땃두릅나무의 광합성 능력 및 광계 II의 활성에 미치는 영향", 한국약용작물학회지, 22(1), pp.38~45 (2014), 한국약용작물학회[DOI]
- Lee YM, "Illustrated rare and endangered species in Korea", pp.171~ (1997), Korea Forest Service
- 이성호, "생물반응기에서 배양한 가시오갈피 유식물체의 순화 및 생육특성", 한국약용작물학회지, 13(4), pp.133~137 (2005), 한국약용작물학회
- Liu J, "Factors influencing seed germination of Oplopanax elatus Nakai", Chinese Bulletin of Botany, 22, pp.183~189 (2005)
- Heung Kyu Moon, "Somatic Embryogenesis and Plantlet Formation from a Rare and Endangered Tree Species, Oplopanax elatus", Journal of Plant Biology(한국식물학회지), 49(4), pp.320~325 (2006), 한국식물학회
- "Korea Forest Service-News and Resources" (2015)[URL]
- 오한준, "해발고도별 반하 종구 무게가 생육 및 수량에 미치는 영향", 한국약용작물학회지, 21(2), pp.130~135 (2013), 한국약용작물학회[DOI]
- Shikov AN, "Oplopanax elatus(Nakai) Nakai: Chemistry, traditional use and pharmacology", Chinese Journal of Natural Medicines, 12, pp.721~729 (2014)
- 송은영, "해발고도별 기온변화에 따른 극조생 양파의 생육 및 구특성", 한국농림기상학회지, 20(4), pp.321~329 (2018), 한국농림기상학회
- Wang JP, "Changbaisanzhi", pp.198~ (1989), Jilin Literature and History Press Books
- 유홍섭, "당귀의 종별 생육특성 및 생산성 비교", 한국약용작물학회지, 12(1), pp.43~46 (2004), 한국약용작물학회
- Zhang HG, "Chemical constituents from stems of Oplopanax elatus Nakai", China Journal of Chinese Materia Medica, 18, pp.104~105 (1993)