옻나무의 추출조건에 따른 페놀성 성분 함량 변화
Effect of Different Extraction Condition on the Contents of Phenolic Compounds in Rhus verniciflua
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Background: In order to develop a method for enhancing the levels of constituents in Rhus verniciflua Stokes extracts, we assessed the effect of ultrasonic thermal fusion treatment and thermal water extraction on the stem of R. verniciflua over different lengths of time. Methods and Results: The phenolic constituents of R. verniciflua stem extracts were analyzed using high-performance liquid chromatogrpahy method. Total phenolic contents were found to be, highest in extracts obtained using ultrasonic thermal fusion treatment for 8 h (URV-8, 12.279%), and thermal water extraction for 4 h (WRV-4, 5.645%). Notably, the total phenolic content of the URV-8 extract was shown to be 2.32 times higher than that of an extract obtained using thermal water extraction for 8 h (WRV-8, 5.295%). Moreover, we found that whereas an extract obtained using ultrasolic thermal fusion for 1 h (URV-1) contained 0.156% urushiol, an allergy-inducing component, no trace of urushiol could be detected after extraction for 2 h. Conclusions: Compared with thermal water extraction, ultrasonic thermal fusion treatment is a more effect method for obtaining plant extracts with high concetrations of phenolic constituetns.
Abstract
Background: In order to develop a method for enhancing the levels of constituents in Rhus verniciflua Stokes extracts, we assessed the effect of ultrasonic thermal fusion treatment and thermal water extraction on the stem of R. verniciflua over different lengths of time. Methods and Results: The phenolic constituents of R. verniciflua stem extracts were analyzed using high-performance liquid chromatogrpahy method. Total phenolic contents were found to be, highest in extracts obtained using ultrasonic thermal fusion treatment for 8 h (URV-8, 12.279%), and thermal water extraction for 4 h (WRV-4, 5.645%). Notably, the total phenolic content of the URV-8 extract was shown to be 2.32 times higher than that of an extract obtained using thermal water extraction for 8 h (WRV-8, 5.295%). Moreover, we found that whereas an extract obtained using ultrasolic thermal fusion for 1 h (URV-1) contained 0.156% urushiol, an allergy-inducing component, no trace of urushiol could be detected after extraction for 2 h. Conclusions: Compared with thermal water extraction, ultrasonic thermal fusion treatment is a more effect method for obtaining plant extracts with high concetrations of phenolic constituetns.
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