Amomum muricarpum Elmer 추출물의 Lipopolysachharide에 의한 염증반응 조절 기전 분석
Immunosuppressive Effects of Amomum muricarpum Elmer in Lipopolysaccharide- triggered Inflammatory Signaling Pathway
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Background: Identifying new natural products to controlling inflammatory responses involved in screening of Asian native plants. The screening revealed a potential candidate, Amomum muricar- pum Elmer, which is a traditional medicinal plant for gastrointestinal diseases in Vietnam; how- ever, its scientific basis remains unknown, its scientific basis remain unknown. Methods and Results: A. muricarpum extract suppressed lipopolysaccharide (LPS)-induced the protein expression of inflammatory mediators such as interleukine (IL)-1β and cyclooxygenase-2 in mouse-derived RAW264.7 macrophages. The mRNA expression levels of IL-6, IL-1β, tumor necrosis factor-α, and inducible nitric oxide synthase in response to LPS were reduced by A. muri- carpum extract. Additionally, A. muricarpum extract inhibited phosphorylation of the signal trans- ducer and activator of transcription 3 (STAT3) and nuclear factor kappa B (NF-κB). This result indicates A. muricarpum suppressed the expression of inflammatory cytokines and mediators by inhibiting signal transducer and STAT3 and NF-κB activation. Conclusions: This is the first scientific evidence to report that A. muricarpum suppresses inflam- matory signaling pathways and can potentially manage excessive and chronic inflammatory responses.
Abstract
Background: Identifying new natural products to controlling inflammatory responses involved in screening of Asian native plants. The screening revealed a potential candidate, Amomum muricar- pum Elmer, which is a traditional medicinal plant for gastrointestinal diseases in Vietnam; how- ever, its scientific basis remains unknown, its scientific basis remain unknown. Methods and Results: A. muricarpum extract suppressed lipopolysaccharide (LPS)-induced the protein expression of inflammatory mediators such as interleukine (IL)-1β and cyclooxygenase-2 in mouse-derived RAW264.7 macrophages. The mRNA expression levels of IL-6, IL-1β, tumor necrosis factor-α, and inducible nitric oxide synthase in response to LPS were reduced by A. muri- carpum extract. Additionally, A. muricarpum extract inhibited phosphorylation of the signal trans- ducer and activator of transcription 3 (STAT3) and nuclear factor kappa B (NF-κB). This result indicates A. muricarpum suppressed the expression of inflammatory cytokines and mediators by inhibiting signal transducer and STAT3 and NF-κB activation. Conclusions: This is the first scientific evidence to report that A. muricarpum suppresses inflam- matory signaling pathways and can potentially manage excessive and chronic inflammatory responses.
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