꾸지뽕나무 잎 추출물의 1,1-Diphenyl-2-picrylhydrazyl Radical 소거 활성 성분 분석
1,1-Diphenyl-2-picrylhydrazyl Radical Scavenging Active Compound Analysis in Extracts of Cudrania tricuspidata leaves
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Background: The 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay is the most commonly used for measuring antioxidant activity. Recently, research on measuring DPPH radical scavenging ability using liquid chromatography (LC) analysis has been increasing. In addition, active compound anal- ysis can be effectively performed using mass spectrometer (MS). Methods and Results: The antioxidant compounds present in the extracts of Cudrania tricuspi- data leaves were analyzed using using an online LC-DPPH radical scavenging activity measure- ment system and MS. The results showed that the DPPH radical scavenging active compound in C. tricuspidata leaves was 6,8-diprenylorobol, a prenylated isoflavonoid. As a result of a comparative analysis of extracts by ethanol concentration, 6,8-diprenylorobol content of the 95% ethanol extract was found to be the highest at 210.63 ± 2.09 ㎍/㎖, while the hot water extract showed the lowest content at 0.06 ± 0.01 ㎍/㎖. Conclusions: 6,8-Diprenylorobol exhibits various physiological activities, including anti-inflam- matory activity, therefore, it can be used as an important marker compound for C. tricuspidata leaves. Additionally, to extract 6,8-diprenylorobol from C. tricuspidata leaves, an ethanol concen- tration of 40% or higher should be used.
Abstract
Background: The 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay is the most commonly used for measuring antioxidant activity. Recently, research on measuring DPPH radical scavenging ability using liquid chromatography (LC) analysis has been increasing. In addition, active compound anal- ysis can be effectively performed using mass spectrometer (MS). Methods and Results: The antioxidant compounds present in the extracts of Cudrania tricuspi- data leaves were analyzed using using an online LC-DPPH radical scavenging activity measure- ment system and MS. The results showed that the DPPH radical scavenging active compound in C. tricuspidata leaves was 6,8-diprenylorobol, a prenylated isoflavonoid. As a result of a comparative analysis of extracts by ethanol concentration, 6,8-diprenylorobol content of the 95% ethanol extract was found to be the highest at 210.63 ± 2.09 ㎍/㎖, while the hot water extract showed the lowest content at 0.06 ± 0.01 ㎍/㎖. Conclusions: 6,8-Diprenylorobol exhibits various physiological activities, including anti-inflam- matory activity, therefore, it can be used as an important marker compound for C. tricuspidata leaves. Additionally, to extract 6,8-diprenylorobol from C. tricuspidata leaves, an ethanol concen- tration of 40% or higher should be used.
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