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커피나무 잎 추출물의 라디칼 소거 활성 성분 탐색

Radical Scavenging Active Compound Screening Analysis in Extract of Coffea arabica L. Leaves

최철희|이경인|표병식|차상화
한국약용작물학회|2022.08|한국약용작물학회지 (KOREAN JOURNAL OF MEDICINAL CROP SCIENCE)|제30권 제4호|pp. 264-277

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참고문헌

키워드

Antioxidant1Mass spectrometryCoffea arabicaLiquid ChromatographyCoffee leaves1-Diphenyl-2-picrylhydrazyl Radical

초록

Background: Coffee tree leaves contain bio-active compounds that exhibit various functions. Recently, coffee trees have been cultivated in Korea, mainly in the south. We analytically investigated the antioxidant activity of coffee tree leaves grown in Korea. Methods and Results: We prepared hot water extracts with a specific extraction time (10 min - 120 min) and ethanol extracts using specific concentration levels (5% - 80%). 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was investigated using liquid chromatography (LC) analysis. We performed a mass spectrometry (MS) using the identified radical scavenging active compound peaks. We confirmed 7 compounds, including 5-caffeoyl quinic acid, 3-caffeoyl quinic acid, cinnamtannin B-1, mangiferin, 3,4-dicaffeoyl quinic acid, 3,5-dicaffeoyl quinic acid, and 4,5-dicaffeoyl quinic acid. The MS with multiple reaction monitoring (MRM) mode analysis provided a method for analyzing radical scavenging active compounds in coffee tree leaf extracts using LC-MS/MS. Conclusions: The comparative analysis revealed, ethanol extraction had a higher extraction efficiency of the antioxidant active compounds than the hot water extraction. The optimum thechnique used over 40% ethanol for extracting the highest levels of antioxidant activity from coffee leaves.

Abstract

Background: Coffee tree leaves contain bio-active compounds that exhibit various functions. Recently, coffee trees have been cultivated in Korea, mainly in the south. We analytically investigated the antioxidant activity of coffee tree leaves grown in Korea. Methods and Results: We prepared hot water extracts with a specific extraction time (10 min - 120 min) and ethanol extracts using specific concentration levels (5% - 80%). 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was investigated using liquid chromatography (LC) analysis. We performed a mass spectrometry (MS) using the identified radical scavenging active compound peaks. We confirmed 7 compounds, including 5-caffeoyl quinic acid, 3-caffeoyl quinic acid, cinnamtannin B-1, mangiferin, 3,4-dicaffeoyl quinic acid, 3,5-dicaffeoyl quinic acid, and 4,5-dicaffeoyl quinic acid. The MS with multiple reaction monitoring (MRM) mode analysis provided a method for analyzing radical scavenging active compounds in coffee tree leaf extracts using LC-MS/MS. Conclusions: The comparative analysis revealed, ethanol extraction had a higher extraction efficiency of the antioxidant active compounds than the hot water extraction. The optimum thechnique used over 40% ethanol for extracting the highest levels of antioxidant activity from coffee leaves.

참고문헌 (39)

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