새싹 율무의 유전 독성 평가
Genotoxicity Evaluation of Coix lacryma-jobi L. var. mayuen Stapf Sprout
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Background: ‘Johyun’ yulmoo which is a new variety of Coix lacryma-jobi var. mayuen Stapf sprout (CS), is the most widely known as medicinal crop for its effects against anti-inflammatory activity. Despite its medicinal activity, there has been no examination of its genotoxicity. Here, we investigated the genotoxicity of CS using Good Laboratory Practice. Methods and Results: An in vitro mammalian chromosomal aberration test, bacterial reverse mutation assay, and micronucleus test were conducted. 1. Chromosome aberration assay : Struc- tural aberrations and aberrant metaphases were not detected at any concentrations of CS tested in this study. 2. Bacterial reverse mutation assay : CS did not induce mutagenicity or affect the num- ber of revertant colonies at any of the tested doses for all five strains for the 50 ㎍/㎖ treated group, with or without metabolic activation with the microsomal enzyme mixture (S9 mixture). 3. Micro- nucleus test : The percentage of micronucleated polychromatic erythrocytes among the total poly- chromatic erythrocytes population compared to the percentage in the negative control group was not affected at any of the CS dosing groups tested. Conclusions: CS did not induce any genotoxicity, as determined by the in vitro chromosome aber- ration test, bacterial mutation assay, and micronucleus test.
Abstract
Background: ‘Johyun’ yulmoo which is a new variety of Coix lacryma-jobi var. mayuen Stapf sprout (CS), is the most widely known as medicinal crop for its effects against anti-inflammatory activity. Despite its medicinal activity, there has been no examination of its genotoxicity. Here, we investigated the genotoxicity of CS using Good Laboratory Practice. Methods and Results: An in vitro mammalian chromosomal aberration test, bacterial reverse mutation assay, and micronucleus test were conducted. 1. Chromosome aberration assay : Struc- tural aberrations and aberrant metaphases were not detected at any concentrations of CS tested in this study. 2. Bacterial reverse mutation assay : CS did not induce mutagenicity or affect the num- ber of revertant colonies at any of the tested doses for all five strains for the 50 ㎍/㎖ treated group, with or without metabolic activation with the microsomal enzyme mixture (S9 mixture). 3. Micro- nucleus test : The percentage of micronucleated polychromatic erythrocytes among the total poly- chromatic erythrocytes population compared to the percentage in the negative control group was not affected at any of the CS dosing groups tested. Conclusions: CS did not induce any genotoxicity, as determined by the in vitro chromosome aber- ration test, bacterial mutation assay, and micronucleus test.
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