해당화 물 추출물의 급성독성 및 유전독성에 관한 연구
Acute Toxicity and Genotoxicity Study of Water Extracts from Rosa rugosa Thunb.
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Backround: Rosa rugosa Thnub. water extract (WRT) has been used as a folk medicine with vari- ous pharmacological properties for a long time in Asia. This study was conducted to evaluate the acute and genetic toxicity of WRT. Method and Result: No test substance-related deaths, clinical signs, weight changes, or macro- scopic manifestations were observed in Sprague-Dawley (SD) rats or Institute of Cancer Research (ICR) mice test animals. WRT did not increase the number of revertant colonies of Salmonella typhimurium or Escherichia coli strains in the presence or absence of metabolic activity. Moreover, it did not induce structurally abnormal chromosomes in Chinese hamster lung (CHL) cells with or without metabolic activity, which did not increase by more than 2-fold compared to that in the neg- ative controls. In the in vivo micronucleus test, WRT did not affect the formation of micronuclei compared with the the negative control. Conclusion: Acute toxicity test for WRT extract in SD rats did not reveal any clinical signs and weight loss. Ames test, in vitro mammalian chromosomal aberration, and in vivo micronucleus test did not reveal WRT genotoxicityt.
Abstract
Backround: Rosa rugosa Thnub. water extract (WRT) has been used as a folk medicine with vari- ous pharmacological properties for a long time in Asia. This study was conducted to evaluate the acute and genetic toxicity of WRT. Method and Result: No test substance-related deaths, clinical signs, weight changes, or macro- scopic manifestations were observed in Sprague-Dawley (SD) rats or Institute of Cancer Research (ICR) mice test animals. WRT did not increase the number of revertant colonies of Salmonella typhimurium or Escherichia coli strains in the presence or absence of metabolic activity. Moreover, it did not induce structurally abnormal chromosomes in Chinese hamster lung (CHL) cells with or without metabolic activity, which did not increase by more than 2-fold compared to that in the neg- ative controls. In the in vivo micronucleus test, WRT did not affect the formation of micronuclei compared with the the negative control. Conclusion: Acute toxicity test for WRT extract in SD rats did not reveal any clinical signs and weight loss. Ames test, in vitro mammalian chromosomal aberration, and in vivo micronucleus test did not reveal WRT genotoxicityt.
참고문헌 (25)
- Ahn MA, "Anti-inflammatory effects of Rosa rugosa extracts in RAW264. 7 cells exposed to particulate matter(PM10)", Journal of Plant Biotechnology, 49, pp.145~149 (2022)
- Altıner D, "The antioxidant effect of Rosa rugosa", Drug Metabol Drug Interact, 23, pp.323~327 (2008)
- van Berlo D, "10% Body weight(gain)change as criterion for the maximum tolerated dose : A critical analysis", Regulatory Toxicology and Pharmacology, 134, pp.105235~ (2022)
- Bringezu F, "Salmonella typhimurium TA100 and TA1535 and E. coli WP2 uvrA are highly sensitive to detect the mutagenicity of short alkyl-N-Nitrosamines in the bacterial reverse mutation test", Toxicology Reports, 9, pp.250~255 (2022)
- Cariello NF, "The Ames test : the two-fold rule revisited", Mutation Research, 369, pp.23~31 (1996)
- Choi HJ, "Safety evaluation from aflatoxin risk of Korean Angelicae Gigantis Radix based on critical control points", Korean Journal of Medical Crop Science, 19, pp.47~53 (2011)
- Galloway SM, "Cytotoxicity and chromosome aberrations in vitro : Experience in industry and the case for an upper limit on toxicity in the aberration assay", Environmental and Molecular Mutagenesis, 35, pp.191~201 (2000)
- Gee P, "Comparison of responses of base-specific Salmonella tester strains with the traditional strains for identifying mutagens : The results of a validation study", Mutation Research, 1998, pp.412, 115~130
- Jung YH, "Antioxidant and anti-inflammatory effects of Ficus erecta var. sieboldii leaf extract in murine macrophage RAW 264. 7 cells", Korean Journal of Plant Resources, 31, pp.303~311 (2018)
- Kang NS, "Immunomodulatory effects of fructus and semen from Rosa rugosa on macrophages", Korean Journal of Plant Resources, 23, pp.399~405 (2010)
- Kim E, "Variations in the antioxidant, anticancer, and anti-inflammatory properties of different Rosa rugosa organ extracts", Agronomy, 12, pp.238~ (2022)
- Kim JW, "Antioxidant activities of hot water extracts from different parts of Rugosa rose(Rosa rugosa Thunb.)", Journal of the Korean Wood Science and Technology, 46, pp.38~47 (2018)
- Kim SH, "Stability test of the extracts of Cimicifugae Rhizoma, Achyranthis Radix, Artemisia Capillaris Herba, Moutan Cortex Radicis and Arecae Semen for toxicity study", Korean Journal of Phamacognosy, 39, pp.241~245 (2008)
- Krishna G, "In vivo rodent micronucleus assay : Protocol, conduct and data interpretation", Mutation Research, 2000, pp.455, 155~166
- Mrózek K, "Encyclopedia of cancer", pp.485~496 (2002), Academic Press
- Kwak M, "Variation in bioactive principles and bioactive compounds of Rosa rugosa fruit during ripening", Journal of Plant Biotechnology, 46, pp.236~245 (2019)
- McCann J, "Detection of carcinogens as mutagens in the Salmonella", microsome test : Assay of 300 chemicals, 1975, pp.72, 5135~5139
- Meijerhof R, "Pre-incubation holding of hatching eggs", World's Poultry Science Journal, 48, pp.57~68 (1992)
- Morton DB, "Guidelines on the recognition of pain, distress and discomfort in experimental animals and an hypothesis for assessment", Veterinary Record, 116, pp.431~436 (1985)
- Mota J, "Humanized mice show clinical signs of dengue fever according to infecting virus genotype", Journal of Virology, 83(17), pp.8638~8645 (2009)
- Nho JH, "Safety evaluation test of galgeun-tang applied to guidelines for the testing of chemicals", Korean Journal of Medicinal Crop Science, 27, pp.173~185 (2019)
- O'brien AD, "Genetic control of susceptibility to Salmonella typhimurium in mice : Role of the LPS gene", The Journal of Immunology, 124, pp.20~21 (1980)
- Park BJ, "Isolation of main component and antioxidant activities on the stem and root of Rosa rugosa", Korean Journal of Plant Resources, 21, pp.402~407 (2008)
- pyrene in vitro by hepatic cytochrome P450 contrasts with detoxification in vivo : Experiments with hepatic cytochrome P450 reductase null mice, "Carcinogenesis", 2008, 656~665
- Levy DD, "Recommended criteria for the evaluation of bacterial mutagenicity data(ames test)", Genetic Toxicology and Environmental Muatagenesis, 848, pp.403074~ (2019)