4 kGy로 감마선 살균처리된 환자용 균형영양식의 유전독성 평가
Genotoxicity evaluation of balanced nutritional food for patients pasteurized by gamma irradiation at 4 kGy
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초록
This study was conducted to evaluate the genotoxicity of balanced nutritional formular for patients containing various ingredients after gamma irradiation at 4 kGy. Since viable bacteria were not observed within the detection limit of 1 log CFU/g, a dose of 4 kGy was appropriate for the pasteurization of the formular. In a bacterial reverse mutation assay, both hot water and methanol extracts of the formular exhibited dose-independent responses, which was similar to those obtained from that of the negative control (distilled water or dimethyl sulfoxide). In a chromosomal aberration test using lung fibroblast cells of Chinese hamster, the numbers of normal chromosomes were comparable to those observed in the negative control, regardless of the treatment dose and metabolic activation system. Furthermore, no significant increases in the frequency of micronucleated polychromatic erythrocytes were observed relative to the control, when mice were fed with the formular at doses up to 2,000 mg/kg body weight. Therefore, the balanced nutritional formular for patients did not exhibit genotoxicity when pasteurization by gamma irradiation at 4 kGy.
Abstract
This study was conducted to evaluate the genotoxicity of balanced nutritional formular for patients containing various ingredients after gamma irradiation at 4 kGy. Since viable bacteria were not observed within the detection limit of 1 log CFU/g, a dose of 4 kGy was appropriate for the pasteurization of the formular. In a bacterial reverse mutation assay, both hot water and methanol extracts of the formular exhibited dose-independent responses, which was similar to those obtained from that of the negative control (distilled water or dimethyl sulfoxide). In a chromosomal aberration test using lung fibroblast cells of Chinese hamster, the numbers of normal chromosomes were comparable to those observed in the negative control, regardless of the treatment dose and metabolic activation system. Furthermore, no significant increases in the frequency of micronucleated polychromatic erythrocytes were observed relative to the control, when mice were fed with the formular at doses up to 2,000 mg/kg body weight. Therefore, the balanced nutritional formular for patients did not exhibit genotoxicity when pasteurization by gamma irradiation at 4 kGy.
참고문헌 (20)
- 2015 production of food and food additives, "Ministry of Food and Drugs Safety", Statistical yearbook of MFDS, ~, pp.MFDS 11-1470000-001922-10 (2016)
- Lee SY, "Manufacture processing of uncooked food on the market", Food Industry and Nutrition, 7, pp.11~15 (2002)
- 김동주, "생식의 위해요인 분석 및 중요관리점 설정", 한국식품과학회지, 36(6), pp.1032~1040 (2004), 한국식품과학회
- Jung K, "Effect of X-ray, gamma ray, and electron beam irradiation on the hygienic and physicochemical qualities of red pepper powder", LWT-Food Sci Technol, 63, pp.846~851 (2015)
- 박시우, "전해수 수세, 열풍건조 및 자외선 조사에 의한 미역의 미생물 감소 효과", 한국미생물·생명공학회지, 43(1), pp.38~44 (2015), 한국미생물·생명공학회[DOI]
- Byun MW, "Application of irradiation technology for food safety and security", Food Science and Industry, 36, pp.25~41 (2003)
- Thayer DW, "Food irradiation: benefits and concerns", J Food Qual, 13, pp.147~169 (1990)
- Farkas J, "History and future of food irradiation", Trends Food Sci Technol, 22, pp.121~126 (2011)
- Kim HC, "A study on the consumers' perception and acceptance toward food irradiation", Korean J Diet Cult, 13, pp.275~291 (1998)
- Wholesomeness of irradiated food, "WHO", FAO, WHO expert committee, pp.1981, ~[DOI]
- High-dose irradiation: Wholesomeness of food irradiated with doses above 10 kGy, "WHO", FAO, WHO study group, pp.1997, ~[DOI]
- 김효정, "방사선 조사 식품에 대한 소비자의 태도", Family and Environment Research, 41(5), pp.119~130 (2003), 대한가정학회
- Organization for Economic Cooperation and Development, "OECD guideline for testing of chemicals No 471: bacterial reverse mutation test", ~ (1997)
- Organization for Economic Cooperation and Development, "OECD guideline for testing of chemicals No. 473: In vitro mammalian chromosome aberration test", ~ (1997)
- Schmid W, "The micronucleus test", Mutat Res Environ Mutagen Relat Subj, 31, pp.9~15 (1975)
- Kwak HJ, "Microbiological and genotoxicological safety of gamma-irradiated chicken", Korean J Food Cookery Sci, 17, pp.617~624 (2001)
- Wakata A, "Measurement of micronuclei by cytokinesis-block method in cultured Chinese hamster cells: comparison with types and rates of chromosome aberrations", Mutat Res Lett, 190, pp.51~57 (1987)
- Kang IJ, "Hygienic quality and genotoxicological safety of gamma irradiated pork", J Korean Soc Food Sci Nutr, 28, pp.1092~1098 (1999)
- 김인보, "한국산 겨우살이 추출물의 안전성 평가", 한국식품영양학회지, 26(3), pp.383~390 (2013), 한국식품영양학회[DOI]
- 육홍선, "감마선 조사된 과메기의 유전독성학적 안전성 평가", 한국식품영양과학회지, 33(1), pp.182~192 (2004), 한국식품영양과학회