본문 바로가기
KERD
논문

건조 방법에 따른 환삼덩굴 추출물 중 Luteolin 유도체의 함량 변화

Changes in Content of Luteolin Derivatives in Humulus japonicus Extracts Prepared using Different Drying Methods

최철희|이경인|표병식
한국약용작물학회|2023.12|한국약용작물학회지 (KOREAN JOURNAL OF MEDICINAL CROP SCIENCE)|제31권 제6호|pp. 414-423

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

Humulus japonicusLiquid ChromatographyLuteolin DerivativeMass Spectroscopy

초록

Background: The main compounds in the Humulus japonicus extract prepared by drying for 12 h under hot air at 50℃ (50-12H), or 7 d, at room temperature at 25℃ (25-7D) were analyzed. Methods and Results: Three main compound peaks were identified in liquid chromatograph anal- ysis using an ultraviolet detector. Additionally, mass spectrometer analysis was performed, and each peak was confirmed to either belong to luteolin 7-O-glucoside, luteolin 4'-O-glucoside, or luteolin through standard analysis. The 50-12H extract showed high contents of luteolin 7-O-glu- coside and luteolin 4’-O-glucoside. Meanwhile, in the 25-7D extract, the luteolin 7-O-glucoside and luteolin 4’-O-glucoside contents reduced and the luteolin content increased, this is because luteolin 7-O-glucoside and luteolin 4’-O-glucoside decomposed into luteolin and glucose by the action of hydrolytic enzymes. In other words, a relatively slow drying speed provides enough time for hydrolytic enzyme to be activiated in H. japonicus, which increases the luteolin content. Conclusions: Room temperature drying is suitable for increasing the luteolin content in H. japoni- cus extract, and a fast heat drying method is suitable for increasing the luteolin glycoside content.

Abstract

Background: The main compounds in the Humulus japonicus extract prepared by drying for 12 h under hot air at 50℃ (50-12H), or 7 d, at room temperature at 25℃ (25-7D) were analyzed. Methods and Results: Three main compound peaks were identified in liquid chromatograph anal- ysis using an ultraviolet detector. Additionally, mass spectrometer analysis was performed, and each peak was confirmed to either belong to luteolin 7-O-glucoside, luteolin 4'-O-glucoside, or luteolin through standard analysis. The 50-12H extract showed high contents of luteolin 7-O-glu- coside and luteolin 4’-O-glucoside. Meanwhile, in the 25-7D extract, the luteolin 7-O-glucoside and luteolin 4’-O-glucoside contents reduced and the luteolin content increased, this is because luteolin 7-O-glucoside and luteolin 4’-O-glucoside decomposed into luteolin and glucose by the action of hydrolytic enzymes. In other words, a relatively slow drying speed provides enough time for hydrolytic enzyme to be activiated in H. japonicus, which increases the luteolin content. Conclusions: Room temperature drying is suitable for increasing the luteolin content in H. japoni- cus extract, and a fast heat drying method is suitable for increasing the luteolin glycoside content.

참고문헌 (40)

  1. 안순철, "당 분해효소를 이용한 감귤 Flavonoid 무배당체 함량의 증가", 한국미생물·생명공학회지, 33(4), pp.288~294 (2005), 한국미생물·생명공학회
  2. 채정우, "채취 시기 및 추출 용매에 따른 환삼덩굴의 항산화 및 기능성 화장품 활성", 한국산림과학회지, 110(2), pp.254~265 (2021), 한국산림과학회[DOI]
  3. 조원준, "Humulus japonicus Extract Ameliorates Hepatic Steatosis Through the PPARα-Mediated Suppression of Alcohol-Induced Oxidative Stress", Journal of Medicinal Food, 26(3), pp.193~200 (2023), 한국식품영양과학회[DOI]
  4. 최정실, "연잎의 품질특성에 미치는 전처리 방법과 저장온도의 영향", Food Science and Preservation, 23(2), pp.155~161 (2016), 한국식품저장유통학회
  5. 최송이, "건조방법과 저장조건이 새싹보리의 대장균 저감과 품질 특성에 미치는 영향", Food Science and Preservation, 29(7), pp.1047~1058 (2022), 한국식품저장유통학회
  6. 엄효진, "건조온도에 따른 비파 잎의 생리활성 변화", 생약학회지, 40(3), pp.178~183 (2009), 한국생약학회
  7. 고준, "Humulus japonicus attenuates LPS-and scopolamine-induced cognitive impairment in mice", Laboratory Animal Research, 38(3), pp.159~168 (2022), 한국실험동물학회[DOI]
  8. 홍민선, "환삼덩굴(Humulus japonicus) 추출물의 항결핵 효과", 한국식품과학회지, 46(1), pp.94~99 (2014), 한국식품과학회[DOI]
  9. 허유정, "건조방법을 달리한 늙은 호박의 품질 특성 및 항산화 효과", 한국식품영양학회지, 31(6), pp.933~939 (2018), 한국식품영양학회[DOI]
  10. Hwang JT, "Efficiency of the enzymatic conversion of flavone glycosides isolated from carrot leaves and anti-inflammatory effects of enzyme-treated carrot leaves", Molecules, 28, pp.4291~ (2023)
  11. Hwang SY, "Anti- inflammatory effects of the MeOH extract of Humulus japonicus in vitro", The Journal of Korean Oriental Medical Ophthalmology and Otolaryngology and Dermatology, 22, pp.92~103 (2009)
  12. Iwashina T, "Further identification of flavonoids deposited in the National Museum of Nature and Science in Japan - Flavonoids isolated from Cirsium Taxa and Carduus nutans (Asteraceae)", Bulletin of the National Museum of Nature and Science, Series B, 46, pp.195~214 (2020)
  13. 정다운, "열풍건조 온도에 따른 고구마 잎 메탄올 추출물의 항산화 효과", Food Science and Preservation, 22(5), pp.708~713 (2015), 한국식품저장유통학회
  14. Chang Muk Kang, "The Aqueous Extract of Humulus japonicus Ameliorates Cognitive Dysfunction in Alzheimer's Disease Models via Modulating the Cholinergic System", Journal of Medicinal Food, 25(10), pp.943~951 (2022), 한국식품영양과학회[DOI]
  15. Kang E, "Humulus japonicus extract ameliorates collagen-induced arthritis in mice through regulation of overall articular inflam-mation", International Journal of Molecular Medicine, 45, pp.417~428 (2020)
  16. 김소진, "주요 생태계 교란 외래식물의 생리활성 비교 평가 및 유효성분", 한국자원식물학회지, 36(4), pp.264~274 (2023), 한국자원식물학회
  17. Kim YB, "Humulus japonicus ameliorates irritant contact dermatitis by suppressing NF-κB p65-dependent inflammatory responses in mice", Experimental and Therapeutic Medicine, 26, pp.446~ (2023)
  18. 김용식, "울금(Curcuma longa L.)의 생산 및 최적 건조조건에 관한 연구", 한국자원식물학회지, 26(4), pp.450~456 (2013), 한국자원식물학회[DOI]
  19. Lech K, "HPLC–UV–ESI MS", MS identification of the color constituents of sawwort(Serratula tinctoria L. ), 2014, pp.406, 3703~3708
  20. Lee HJ, "Dereplication of components coupled with HPLC-qTOF-MS in the active fraction of Humulus japonicus and it's protective effects against Parkinson's disease mouse model", Molecules, 24, pp.1435~ (2019)
  21. 이주민, "건조방법에 따른 한련초 잎의 영양성분 및 항산화활성 비교", 한국지역사회생활과학회지, 29(1), pp.59~68 (2018), 한국지역사회생활과학회[DOI]
  22. Lin Y, "Luteolin-4′-O-glucoside and its aglycone, two major flavones of Gnaphalium affine D. Don, resist hyperuricemia and acute gouty arthritis activity in animal models", Phytomedicine, 41, pp.54~61 (2018)
  23. Eui Jeong Nam, "Glycosyl flavones from Humulus japonicus suppress MMP-1 production via decreasing oxidative stress in UVB irradiated human dermal fibroblasts", BMB Reports, 53(7), pp.379~384 (2020), 생화학분자생물학회[DOI]
  24. 박건희, "효소 처리 시간에 따른 진피의 플라보노이드 비배당체 함량 및 항산화 활성 비교", 한국식품영양과학회지, 48(5), pp.542~550 (2019), 한국식품영양과학회
  25. 박혜수, "아토피성 피부로 건조함을 가진 대상자에 대한 환삼덩굴추출물 함유 기능성 화장품의 유효성 및 안전성을 평가하기 위한 임상적 연구", 한방안이비인후피부과학회지, 32(2), pp.24~58 (2019), 대한한방안이비인후피부과학회[DOI]
  26. Park KY, "Inhibitory effect of luteolin 4'-O-glucoside from Kummerowia striata and other flavonoids on interleukin-5 bioactivity", Planta Medica, 65, pp.457~459 (1999)
  27. Park SW, "Active oxygen scavenging activity of luteolin-7-O-β-D-glucoside isolated from Humulus japonicus", Journal of the Korean Society of Food Science and Nutrition, 29, pp.106~110 (2000)
  28. Park SW, "Antimutagenic effects and isolation of flavonoids from Humulus japonicus extract", Korean Journal of Food Science and Technology, 27, pp.897~901 (1995)
  29. 류가희, "금전초에서 분리한 cynaroside의 신경세포보호 활성", 생약학회지, 54(1), pp.9~15 (2023), 한국생약학회
  30. Shin H, "Identification of Polygonum orientale constituents using high- performance liquid chromatography high-resolution tandem mass spectrometry", Bioscience, Biotechnology, and Biochemistry, 82, pp.15~21 (2018)
  31. 신광섭, "Effect of commercial carbohydrases on the hesperetin and narigenin contents of citrus fruits", Food Science and Preservation, 27(4), pp.446~456 (2020), 한국식품저장유통학회
  32. Slámová K, "“Sweet flavonoids”: Glycosidase-catalyzed modifications", International Journal of Molecular Sciences, 19, pp.2126~ (2018)
  33. Sung BK, "Humulus japonicus extract exhibits antioxidative and anti-aging effects via modulation of the AMPK-SIRT1 pathway", Experimental and Therapeutic Medicine, 9, pp.1819~1826 (2015)
  34. 성화정, "함초의 건조방법에 따른 항산화 및 항혈전 활성의 변화", Food Science and Preservation, 24(5), pp.658~665 (2017), 한국식품저장유통학회
  35. Wang F, "Anti- neuroinflammatory activity of Humulus japonicus extract and its active compound luteolin on lipopolysaccharide-induced inflammatory response in SIM-A9 microglia via NF-κB and MAPK signaling pathways", Food Science and Technology, 42, pp.e78621~ (2022)
  36. Wang F, "Antioxidative stress effects of Humulus japonicus extracts on neuronal PC12 cells", Food Science and Technology, 42, pp.e101921~ (2022)
  37. Xu T, "Glycosylation of luteolin in hydrophilic organic solvents and structure-antioxidant relationships of luteolin glycosides", RSC Advances, 12, pp.18232~18237 (2022)
  38. 윤광섭, "건조방법에 따른 여주의 품질특성 및 항산화 활성", Food Science and Preservation, 26(2), pp.185~193 (2019), 한국식품저장유통학회
  39. Yuan S, "Isolation and characterization of a multifunctional flavonoid glycosyltransferase from Ornithogalum caudatum with glycosidase activity", Scientific Reports, 8, pp.5886~ (2018)
  40. 윤정민, "건조방법에 따른 사과꽃 5종의 항산화 활성 및 HPLC를 활용한 정성․정량분석", Food Science and Preservation, 28(6), pp.780~789 (2021), 한국식품저장유통학회

관련 논문AI추천

금은화의 폴리페놀, 플라보노이드, Chlorogenic acid의 최적 추출조건김명기, 송선영, 하헌용공학기술연구원 · 공학기술논문지2018 · 제11권 제1호유채꽃 가공유형별 플라보노이드 조성 및 함량 변화김선주, 이기택, 이영화, 임예훈, 천진혁, 홍순택한국환경농학회 · 한국환경농학회지2017 · 제36권 제1호황기의 추출조건 및 유효성분의 HPLC 분석법 평가김금숙, 김승유, 노형준, 박춘근, 이대영, 이승은, 최수임, 최재훈, 홍승재한국약용작물학회 · 한국약용작물학회지2013 · 제21권 제6호전탕시간에 따른 애엽의 성분패턴 비교연구김민선, 윤준걸, 이진무, 이창훈, 장준복, 한성민, 황덕상대한한방부인과학회 · 대한한방부인과학회지2020 · 제33권 제2호Glycosyl flavones from Humulus japonicus suppress MMP-1 production via decreasing oxidative stress in UVB irradiated human dermal fibroblasts노주원, Changho Jhin, Eui Jeong Nam, Gyhye Yoo, Joo Young Lee, Myungsuk Kim, Sang Hoon Jung, Sun Young Kim, Yang-Ju Son생화학분자생물학회 · BMB Reports2020 · 제53권 제7호건조방법에 따른 국내 자생 쑥부쟁이속 5종의 항산화 및 항염 활성 비교박경태, 이승연, 이하민, 장보국, 조주성한국자원식물학회 · 한국자원식물학회지2026 · 제39권 제1호

이 간행물의 다른 논문 (6건)

일천궁에 발생하는 오이모자이크바이러스의 현장 검출을 위한 Lateral Flow Reverse Transcription Recombinase Polymerase Amplification (LF-RT-RPA) 개발이현택, 윤주연, 주호종pp. 359-370단삼 신품종 ‘다산 ’의 재배 및 품질특성마경호, 이우문, 이정훈, 이윤지, 김영국, 안태진, 정진태, 허목, 한종원pp. 371-376냉이의 연구 현황 및 활용 가능성박소영, 이시철, 권수진, 하기훈, 이종열, 이수인, 김진아pp. 377-387결명자 새싹의 재배 방법과 수확 시기에 따른 항산화 활성이문순, 강용구, 이영섭, 이윤지, 김관우, 양창열, 정양선, 전제승pp. 388-395육묘 조건이 당귀속 2 종 약용작물의 수량 및 묘 품질에 미치는 영향김민석, 한경숙, 김용일, 김용구, 이은송pp. 396-406
원문 보기