Characterization of Expressed Sequence Tags (ESTs) Generated from the Bombyx mandarina Whole Larvae and Molecular Cloning of Serine Protease Homologue Gene
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Abstract
We constructed an oligo-d(T) primed directional cDNA library from the Bombyx mandarina whole larvae. In an effort to isolate genes expressed in the B. mandarina, 227 expressed sequence tags (ESTs) were generated by single-pass sequencing from the cDNA library. Sequence analysis showed that 107 clones (47.1%) were classified into known genes and 120 clones (52.9%) were novel transcripts, which are unknown for their function. Of the 107 known genes, the most abundant gene was found to be actin and followed by serine protease in the expression profile. Among these clones, a serine protease homolog (BmSP) which is a class of proteolytic enzymes isolated. Full-length sequence of the BmSP cDNA clone was 922 bp in length and has an open reading frame of 276 amino acids. The conserved histidine, aspatic acid and serine residues forming the catalytic center as well as cysteine residues contributing to three disulphide bonds also were found in Bmsp gene. mRNA expression analysis revealed a high and specific expression of the gene only in midgut tissue, suggesting that BmSP gene is closely associated with the expression of digestive enzyme.
참고문헌 (18)
- "Initial assessment of human gene diversity and expressed patterns based upon 83 million nucleotides of cDNA sequence.", 377, pp.3~7 (1995)
- "cDNA and deduced amino acid sequence of a blood mealinducedtrypsin from the mosquito", pp.825~831 (1991)
- "activationand regulation of the first complement component", lmmu(l), pp.151~216 (1985)
- "Introduction to the blood coagulation cascadeand the cloning of blood coagulation factors", pp.247~253 (1986)
- "Agene required for the specificationof dorsal- ventral pattern in Drosophila appears toencode a serine protease", pp.688~323 692 (1986)
- "generation of diversity of neuroendocrine peptides", pp.665~715 (1984), Polyprotein geneexpression
- "The sequence 5-AAUAAA-3 forms parts of the recognition site for polyadenyllationof late SV40 mRNAs", pp.251~260 (1981)
- "Isolation of trypsin isozymesfrom the mosquito Aedes aegypti", pp.611~618 (1985)
- "Generation and analysis of 280,000 human expressed sequence tags", 6, pp.807~828 (1996)
- "The Genexpress Index: A resource for gene discovery and the gentic map of the human genome", 5, pp.272~304 (1995)
- "Amino and sequence of an insect chymotrypsin from the larvaeof the hornet", pp.1~7 (1983)
- "sequencing andcharacterization of two cDNA clones coding for trypsin-likeenzymes from the midgut of Aedes aegypti", pp.71~79 (1993)
- "Assessment of human gene diversity andexpression pattern using expressed sequence tags", 31, pp.1~9 (1996)
- "Gene expression in ratbrain", pp.5497~54520 (1983)
- "Members of a trypsin gene family in Anophelesgambiae are induced in the gut by blood meal", pp.2891~2900 (1993)
- "A summary of methods foraccessing results from large-scale partial sequencing ofanonymous aravidopsis cDNA clones", pp.1241~1225 (1994)
- "A newtroponin T and cDNA clones for 13 different muscle proteins found by shotgun sequencing", pp.718~302 721 (1983)
- "Large-scalesequencing of a normalized cDNA library", murauama-kaya, pp.9228~91 9232 (1994)