Inhibitory Effect of the Culture Broth Extract of Aspergillus tamarii on Nitric Oxide Production and Its Antioxidative Activity
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Many studies have explored suppression of aflatoxin produced by Aspergillus Genus. On the other hand, this study examined the inhibitory effect of the culture broth extract (CE) of A. tamarii obtained from dead silkworm on nitric oxide (NO) production and its antioxidative activity. The culture broth was extracted with EtOAc,dried, and then used in this experiment. As a result, CE did not show cytotoxicity on RAW 264.7 cells at any concentration. Moreover, CE suppressed lipopolysaccharide (LPS)-induced NO production of RAW 264.7 cells in a dose-dependent manner. The total phenol content according to the Folin-Dennis method, the antioxidative activity by DPPH, and the nitrate radical scavenging capacity of CE were increased in a dose-dependent manner. Thus, many of the phenolic compounds were considered to represent the antioxidative activity.
Abstract
Many studies have explored suppression of aflatoxin produced by Aspergillus Genus. On the other hand, this study examined the inhibitory effect of the culture broth extract (CE) of A. tamarii obtained from dead silkworm on nitric oxide (NO) production and its antioxidative activity. The culture broth was extracted with EtOAc,dried, and then used in this experiment. As a result, CE did not show cytotoxicity on RAW 264.7 cells at any concentration. Moreover, CE suppressed lipopolysaccharide (LPS)-induced NO production of RAW 264.7 cells in a dose-dependent manner. The total phenol content according to the Folin-Dennis method, the antioxidative activity by DPPH, and the nitrate radical scavenging capacity of CE were increased in a dose-dependent manner. Thus, many of the phenolic compounds were considered to represent the antioxidative activity.
참고문헌 (19)
- Chang LP, "Liquid perfluorochemical inhibits inducible nitric oxide synthase expression and nitric oxide formation in lipopolysaccharide-treated RAW 264.7 macrophages", J Pharmacol Sci, 111, pp.147~154 (2009)
- Choe SY, "Toxicological studies of antioxidants, butylated hydroxytoluene (BHT) and butlylated hydroxyanisole (BHA)", Kor J Food Sci Technol, 14, pp.283~288 (1982)
- Doan T, "Lippincott's illustrated reviews: Immunology" (2008), Lippincott Williams & Wilkins
- Horn BW, "The sexual state of Aspergillus parasiticus", Mycologia, 101, pp.275~280 (2009)
- Huang SJ, "Nonvolatile taste components of fruit bodies and mycelia of Cordyceps militaris", LWT, 39, pp.577~583 (2006)
- Hwang PA, "Inhibition of lipopolysaccharide (LPS)-induced inflammatory responses by Sargassum hemiphyllum sulfated polysaccharide extract in RAW 264.7 macrophage cells", J Agric Food Chem, 59, pp.2062~2068 (2011)
- Kang KJ, "Inhibition of aflatoxin-producing fungi with antifungal compound produced by Bacillus subtilis", J Fd Hyg Safety, 15, pp.122~127 (2000)
- 김종욱, "Aspergillus속과 Bacillus subtilis를 이용한 된장메주 발효 중 품질 특성", 한국식품저장유통학회지, 18(3), pp.397~406 (2011), 한국식품저장유통학회
- Kindt TJ, "Kuby immunology" (2007), W.H. Freeman
- Kumar V, "Robbins basic pathology" (2007), Elsevier
- Lee HO, "Antibacterial effects of Sophora flavescens on Streptococcus mutans", Kor J Food Nutr, 13, pp.539~546 (2000)
- 이기만, "번데기동충하초와 눈꽃동충하초의 배양특성 및 항균활성", 한국응용곤충학회지, 47(4), pp.479~486 (2008), 한국응용곤충학회
- Liu WJH, "Traditional herbal medicine research methods" (2011), John Wiley & Sons
- Martins HM, "Interaction of wild strains of Aspergilla with Aspergillus parasiticus ATCC15517 and aflatoxin production", Int J Mol Sci, 9, pp.394~400 (2008)
- Nam SH, "Genetic relationship of Cordyceps spp. based on internal transcribed spacer sequence of ribosomal DNA", Kor J Seri Sci, 41, pp.174~179 (1999)
- Nam SH, "Occurrence and characteristics of other fungi in the artificial cultivation farms of Paecilomyces tenuipes", Kor J Seric Sci, 44, pp.32~36 (2002)
- Samson RA, "Atlas of entomopathogenic fungi" (1988), Springer
- Sung JM, "Classification of Cordyceps species based on protein banding pattern", Kor J Mycol, 26, pp.1~7 (1998)
- Whang HJ, "Quantitative analysis of total phenolic content in apple", Analytical Sci Technol, 14, pp.377~383 (2001)