Effect of Residual Lithium Ions on the Structure and Cytotoxicity of Silk Fibroin Film
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Dialysis is the rate-limiting step in the preparation of aqueous silk fibroin (SF) solution. However, the traditional practice of dialyzing SF solution for at least 48 h to remove LiBr is notbased on empirical evidence. The aim of the present study was to systematically measure LiBrcontent in SF solutions dialyzed for varying lengths of time and assess the potential toxicity ofresidual lithium ions in cells. Complete removal of lithium ions was not achieved even after 72h of dialysis, with a residual lithium ion content in the solution of 22.85 mg/l. SF films preparedfrom solutions dialyzed for 8 and 24 h had predominantly random coil or b-sheet structures,respectively. The residual lithium had little cytotoxicity in NIH3T3 fibroblast cells, but viabilitywas compromised in cells grown on SF film prepared from solution dialyzed for 24 h.
Abstract
Dialysis is the rate-limiting step in the preparation of aqueous silk fibroin (SF) solution. However, the traditional practice of dialyzing SF solution for at least 48 h to remove LiBr is notbased on empirical evidence. The aim of the present study was to systematically measure LiBrcontent in SF solutions dialyzed for varying lengths of time and assess the potential toxicity ofresidual lithium ions in cells. Complete removal of lithium ions was not achieved even after 72h of dialysis, with a residual lithium ion content in the solution of 22.85 mg/l. SF films preparedfrom solutions dialyzed for 8 and 24 h had predominantly random coil or b-sheet structures,respectively. The residual lithium had little cytotoxicity in NIH3T3 fibroblast cells, but viabilitywas compromised in cells grown on SF film prepared from solution dialyzed for 24 h.
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