A preliminary study of the anti-inflammatory activities of the Japanese oak silk moth, Antheraea yamamai
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초록
The present study aimed to determine whether a hemolymph prepared from Antheraea yamamai larvae had the same biological activities using a Bombyx mori hemolymph prior to exposure to lipopolysaccharide (LPS) in order to induce an inflammatory response. The effects of the hemolymph were determined using a reverse transcription‑quantitative polymerase chain reaction to assess the expression of pro‑inflammatory molecules. The A. yamamai hemolymph exerted anti‑inflammatory effects on LPS‑activated human monocytic leukemia cells via Toll-like receptor (TLR) 4‑mediated suppression, similar to the B. mori hemocyte extract. Treatment with the A. yamamai hemolymph significantly suppressed LPS‑induced upregulated inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) mRNA expression at all tested concentrations compared with the control, similar to the B. mori immune-challenged hemolymph. Finally, the A. yamamai hemolymph, like the B. mori immune-challenged hemolymph, suppressed all of these concentrations in a dose‑independent manner. These results demonstrate that the hemolymph of A. yamamai exhibited important biologically active substances. Further in‑depth functional studies are required to fully understand the mechanisms underlying the biological activities of wild-type silkworm hemolymphs.
Abstract
The present study aimed to determine whether a hemolymph prepared from Antheraea yamamai larvae had the same biological activities using a Bombyx mori hemolymph prior to exposure to lipopolysaccharide (LPS) in order to induce an inflammatory response. The effects of the hemolymph were determined using a reverse transcription‑quantitative polymerase chain reaction to assess the expression of pro‑inflammatory molecules. The A. yamamai hemolymph exerted anti‑inflammatory effects on LPS‑activated human monocytic leukemia cells via Toll-like receptor (TLR) 4‑mediated suppression, similar to the B. mori hemocyte extract. Treatment with the A. yamamai hemolymph significantly suppressed LPS‑induced upregulated inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) mRNA expression at all tested concentrations compared with the control, similar to the B. mori immune-challenged hemolymph. Finally, the A. yamamai hemolymph, like the B. mori immune-challenged hemolymph, suppressed all of these concentrations in a dose‑independent manner. These results demonstrate that the hemolymph of A. yamamai exhibited important biologically active substances. Further in‑depth functional studies are required to fully understand the mechanisms underlying the biological activities of wild-type silkworm hemolymphs.
참고문헌 (10)
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