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Isolation and Characterization of Plutella xylostella Granulovirus Isolated in Korea

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Abstract

We have first isolated and characterized a Plutella xylostella granulovirus (PxGV) from dead larvae of P. xylostella in Korea. The granule of PxGV was ovoidal shape with an approximate measure of 350 − 400 nm × 150 − 200 nm, and each granule contained one single rod-shape virion with a mean size of 150 − 180 nm × 20 − 30 nm. The major granule protein, granulin, had a molecular weight of approximately 29 kDa. Whereas the nucleotide sequence of the granulin gene was identical to that of previously reported PxGV, nucleotide sequences of two of three putative p10 genes were slightly different from those of reported PxGV. These results suggested that the PxGV isolated in this study was a novel isolate containing different genomic information.

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